Binding of guanylyl cyclase activating protein 1 (GCAP1) to retinal guanylyl cyclase (RetGC1). The role of individual EF-hands.
AuthorsPeshenko IV, Olshevskaya EV, Dizhoor AM,
JournalJ Biol Chem
PubMed ID18541533
'Guanylyl cyclase activating protein 1 (GCAP1), after substitution of Ca(2+) by Mg(2+) in its EF-hands, stimulates photoreceptor guanylyl cyclase, RetGC1, in response to light. We inactivated metal binding in individual EF-hands of GCAP1 tagged with green fluorescent protein to assess their role in GCAP1 binding to RetGC1 in co-transfected HEK293 ... More
Specific effects of surface amines on polystyrene nanoparticles in their interactions with mesenchymal stem cells.
AuthorsJiang X, Dausend J, Hafner M, Musyanovych A, Röcker C, Landfester K, Mailänder V, Nienhaus GU,
JournalBiomacromolecules
PubMed ID20166675
'We have investigated the uptake of cationic polystyrene nanoparticles by mesenchymal stem cells (MSCs) using confocal fluorescence microscopy and flow cytometry. Two types of nanoparticles of about 100 nm diameter with similar zeta potentials were employed in this study, plain polystyrene (PS) nanoparticles and amino-functionalized polystyrene (NPS) nanoparticles, each carrying ... More
Accumulation of amyloid-like Aß1-42 in AEL (autophagy-endosomal-lysosomal) vesicles: potential implications for plaque biogenesis.
AuthorsLing D, Magallanes M, Salvaterra PM,
Journal
PubMed ID24521233
'Abnormal accumulation of Aß (amyloid ß) within AEL (autophagy-endosomal-lysosomal) vesicles is a prominent neuropathological feature of AD (Alzheimer''s disease), but the mechanism of accumulation within vesicles is not clear. We express secretory forms of human Aß1-40 or Aß1-42 in Drosophila neurons and observe preferential localization of Aß1-42 within AEL vesicles. ... More
Insights into the residence in lipid rafts of adenylyl cyclase AC8 and its regulation by capacitative calcium entry.
AuthorsPagano M, Clynes MA, Masada N, Ciruela A, Ayling LJ, Wachten S, Cooper DM,
JournalAm J Physiol Cell Physiol
PubMed ID19158400
'Adenylyl cyclases (ACs) are a family of critically important signaling molecules that are regulated by multiple pathways. Adenylyl cyclase 8 (AC8) is a Ca(2+) stimulated isoform that displays a selective regulation by capacitative Ca(2+) entry (CCE), the process whereby the entry of Ca(2+) into cells is triggered by the emptying ... More
Enterotoxigenic Escherichia coli secretes a highly conserved mucin-degrading metalloprotease to effectively engage intestinal epithelial cells.
AuthorsLuo Q, Kumar P, Vickers TJ, Sheikh A, Lewis WG, Rasko DA, Sistrunk J, Fleckenstein JM,
Journal
PubMed ID24478067
'Enterotoxigenic Escherichia coli (ETEC) is a leading cause of death due to diarrheal illness among young children in developing countries, and there is currently no effective vaccine. Many elements of ETEC pathogenesis are still poorly defined. Here we demonstrate that YghJ, a secreted ETEC antigen identified in immunoproteomic studies using ... More
RAGE inhibits human respiratory syncytial virus syncytium formation by interfering with F-protein function.
'Human respiratory syncytial virus (RSV) is a major cause of severe lower respiratory tract infection. Infection is critically dependent on the RSV fusion (F) protein, which mediates fusion between the viral envelope and airway epithelial cells. The F protein is also expressed on infected cells and is responsible for fusion ... More
Infant satiety depends on transient expression of cholecystokinin-1 receptors on ependymal cells lining the third ventricle in mice.
AuthorsOzaki T, Mohammad S, Morioka E, Takiguchi S, Ikeda M,
JournalJ Physiol
PubMed ID23266937
'Cholecystokinin (CCK) is a hypothetical controller for suckling and infancy body weight, although the underlying mechanisms remain unclear. Therefore, the present study analysed the mechanisms using mice lacking the CCK-1 receptor (CCK1R-/-). Although CCK1R-/- mice displayed normal weights at birth and adulthood, CCK1R-/- pups had enlarged adipocytes and were overweight ... More
Stationary phase and nutrient levels trigger transcription of a genomic locus containing a novel peptide (TM1316) in the hyperthermophilic bacterium Thermotoga maritima.
AuthorsFrock AD, Montero CI, Blumer-Schuette SE, Kelly RM,
Journal
PubMed ID23974142
The genome of the hyperthermophilic bacterium Thermotoga maritima encodes numerous putative peptides/proteins of 100 amino acids or less. While most of these open reading frames (ORFs) are transcribed during growth, their corresponding physiological roles are largely unknown. The onset of stationary phase in T. maritima was accompanied by significant morphological ... More
Role of macrophage receptor with collagenous structure in innate immune tolerance.
AuthorsJing J, Yang IV, Hui L, Patel JA, Evans CM, Prikeris R, Kobzik L, O'Connor BP, Schwartz DA,
Journal
PubMed ID23667110
Macrophages play a key role in host defense against microbes, in part, through phagocytosis. Macrophage receptor with collagenous structure (MARCO) is a scavenger receptor on the cell surface of macrophages that mediates opsonin-independent phagocytosis. The goal of our study is to investigate the role of MARCO in LPS or lipotechoic ... More
Particle shape enhances specificity of antibody-displaying nanoparticles.
Monoclonal antibodies are used in numerous therapeutic and diagnostic applications; however, their efficacy is contingent on specificity and avidity. Here, we show that presentation of antibodies on the surface of nonspherical particles enhances antibody specificity as well as avidity toward their targets. Using spherical, rod-, and disk-shaped polystyrene nano- and ... More
Mechanics of spreading cells probed by atomic force microscopy.
AuthorsPietuch A, Janshoff A,
Journal
PubMed ID23864554
Cellular adhesion and motility are fundamental processes in biological systems such as morphogenesis and tissue homeostasis. During these processes, cells heavily rely on the ability to deform and supply plasma membrane from pre-existing membrane reservoirs, allowing the cell to cope with substantial morphological changes. While morphological changes during single cell ... More
Magnetic fractionation and proteomic dissection of cellular organelles occupied by the late replication complexes of semliki forest virus.
AuthorsVarjak M, Saul S, Arike L, Lulla A, Peil L, Merits A,
Journal
PubMed ID23864636
Alphavirus replicase complexes are initially formed at the plasma membrane and are subsequently internalized by endocytosis. During the late stages of infection, viral replication organelles are represented by large cytopathic vacuoles, where replicase complexes bind to membranes of endolysosomal origin. In addition to viral components, these organelles harbor an unknown ... More
Human B cells differentiate into granzyme B-secreting cytotoxic B lymphocytes upon incomplete T-cell help.
AuthorsHagn M, Sontheimer K, Dahlke K, Brueggemann S, Kaltenmeier C, Beyer T, Hofmann S, Lunov O, Barth TF, Fabricius D, Tron K, Nienhaus GU, Simmet T, Schrezenmeier H, Jahrsdörfer B,
JournalImmunol Cell Biol
PubMed ID21808264
Recently, CD4(+) T helper cells were shown to induce differentiation of human B cells into plasma cells by expressing interleukin (IL-)21 and CD40 ligand (CD40L). In the present study we show, that in the absence of CD40L, CD4(+) T cell-derived IL-21 induces differentiation of B cells into granzyme B (GzmB)-secreting ... More
Microfluidic-based cell sorting of Francisella tularensis infected macrophages using optical forces.
AuthorsPerroud TD, Kaiser JN, Sy JC, Lane TW, Branda CS, Singh AK, Patel KD,
JournalAnal Chem
PubMed ID18510341
We have extended the principle of optical tweezers as a noninvasive technique to actively sort hydrodynamically focused cells based on their fluorescence signal in a microfluidic device. This micro fluorescence-activated cell sorter (microFACS) uses an infrared laser to laterally deflect cells into a collection channel. Green-labeled macrophages were sorted from ... More
Apoptotic Extracellular Vesicles Ameliorate Multiple Myeloma by Restoring Fas-Mediated Apoptosis.
Authors
JournalACS Nano
PubMed ID34506129
Mitochondrial fragmentation drives selective removal of deleterious mtDNA in the germline.
Authors
JournalNature
PubMed ID31092924
Three-Dimensional Two-Color Dual-Particle Tracking Microscope for Monitoring DNA Conformational Changes and Nanoparticle Landings on Live Cells.
Authors
JournalACS Nano
PubMed ID32668152
The lipolysis pathway sustains normal and transformed stem cells in adult Drosophila.
Authors
JournalNature
PubMed ID27680705
Non-canonical autophagy drives alternative ATG8 conjugation to phosphatidylserine.
Authors
JournalMol Cell
PubMed ID33909989
Inceptor counteracts insulin signalling in β-cells to control glycaemia.
Authors
JournalNature
PubMed ID33505018
Prohibitin 2 Is an Inner Mitochondrial Membrane Mitophagy Receptor.
Authors
JournalCell
PubMed ID28017329
Photothermal nanofibres enable safe engineering of therapeutic cells.
Authors
JournalNat Nanotechnol
PubMed ID34675410
Nanotopography Enhances Dynamic Remodeling of Tight Junction Proteins through Cytosolic Liquid Complexes.
Authors
JournalACS Nano
PubMed ID32940450
Eradication of Triple-Negative Breast Cancer Cells by Targeting Glycosylated PD-L1.