Chick embryo proliferation studies using EdU labeling.
AuthorsWarren M, Puskarczyk K, Chapman SC,
JournalDev Dyn
PubMed ID19253396
Cell proliferation studies are an important experimental tool. The most commonly used thymidine analogues, tritiated thymidine and bromodeoxyuridine (BrdU) label cells during S-phase. Both methods have significant drawbacks: low sensitivity in the case of tritiated thymidine and a denaturation step during BrdU detection that destroys most cellular epitopes, requiring careful ... More
A rapid non-radioactive technique for measurement of repair synthesis in primary human fibroblasts by incorporation of ethynyl deoxyuridine (EdU).
AuthorsLimsirichaikul S, Niimi A, Fawcett H, Lehmann A, Yamashita S, Ogi T,
JournalNucleic Acids Res
PubMed ID19179371
Xeroderma pigmentosum (XP) is an autosomal recessive genetic disorder. Afflicted patients show extreme sun-sensitivity and skin cancer predisposition. XP is in most cases associated with deficient nucleotide excision repair (NER), which is the process responsible for removing photolesions from DNA. Measuring NER activity by nucleotide incorporation into repair patches, termed ... More