Click-iT™ EdU 成像试剂盒，包含 Alexa Fluor™ 488、594 和 647 叠氮化物

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Invitrogen™

Click-iT™ EdU 成像试剂盒，包含 Alexa Fluor™ 488、594 和 647 叠氮化物

Click-iT™ EdU 成像试剂盒是传统增殖检测的卓越替代方法，是我们针对荧光显微镜应用进行过优化的试用版产品。在该检测中，将经修饰的胸苷类似物 EdU 有效地掺入新合成的 DNA 中，并在快速、高度特异性的 click了解更多信息

货号	数量
C10086	1 kit

货号 C10086

价格（CNY)

959.00

Ends: 31-Dec-2025

1,298.00

共减 339.00 (26%)

添加至购物车

1 kit

价格（CNY)

959.00

Ends: 31-Dec-2025

1,298.00

共减 339.00 (26%)

添加至购物车

Click-iT™ EdU 成像试剂盒是传统增殖检测的卓越替代方法，是我们针对荧光显微镜应用进行过优化的试用版产品。在该检测中，将经修饰的胸苷类似物 EdU 有效地掺入新合成的 DNA 中，并在快速、高度特异性的 click 反应中用明亮的、光稳定的 Alexa Fluor™ 染料进行荧光标记。得益于温和的 click 方案，这种增殖细胞的荧光标记非常准确并且与抗体方法兼容。该试用版试剂盒可提供足以标记多达 6–30 张盖玻片的 EdU，并提供足够的 Alexa Fluor™ 488、594和647叠氮化物，以便使用每种荧光基团检测 2–10 张盖玻片上的新 DNA 合成。
•简单—每次都能在更短的时间内完成工作
• 高效—无需变性步骤或严苛处理
• 丰富的检测结果—更好地保存细胞形态、抗原结构和 DNA 完整性
• 一致—不依赖于可变的抗体批次进行检测

准确的细胞增殖检测方法是基于核苷类似物在新合成 DNA 中的掺入及测量，其中溴脱氧尿苷 (BrdU) 是一种常用的类似物。通过严苛处理方法（HCl、加热或酶）使 DNA 变性并暴露于 BrdU 分子后，使用抗 BrdU 抗体对 BrdU 标记的 DNA 进行定量检测。此步骤耗时且难以一致地执行。严苛处理也会对样品的完整性和质量产生不利影响，这使得与其他抗体的共染色具有挑战性。

卓越的增殖方法学
Click-iT™ EdU 成像试剂盒可提供一种优于 BrdU 检测的细胞增殖检测方法。EdU（5-乙基-2'-脱氧尿苷）是脱氧胸腺嘧啶的核苷类似物，在活跃的 DNA 合成过程中掺入 DNA 中。使用 Click-iT™ EdU，温和的固定和洗涤剂透化足以使基于小分子的 Click-iT™ EdU 检测试剂获得掺入 DNA 的路径。因此，Click-iT™ EdU 成像试剂盒不仅简便易用，而且更准确且与细胞周期分析以及其他的细胞内或细胞外目标成分相兼容，以获得令人满意且丰富的检测结果。

该试剂盒针对荧光显微镜应用进行了优化；如果需要专为高内涵成像、荧光微孔板检测或流式细胞分析平台设计的试剂盒，请访问本公司网站的 Click-iT™ 技术部分。

进一步了解 Click-iT™ 技术

注释：
通过补料或注射方法给予 EdU 后，Click-iT™ 测定试剂盒可用于培养的细胞或体内的细胞。
通过使用不与 EdU 发生交叉反应的抗 BrdU（克隆 MoBu-1）抗体，可将 Click-iT™ 测定试剂盒与 BrdU 一起用于双脉冲实验。
Click-iT™ 技术与具有固定耐受性或设计用于固定细胞标记的免疫组织化学、免疫细胞化学以及荧光染料相兼容。

仅供科研使用。不可用于诊断程序。

细胞类型哺乳动物细胞、精子细胞、真菌细胞、真核细胞、细菌、酵母细胞

检测方法荧光

染料类型Alexa Fluor™ 488、Alexa Fluor™ 594、Alexa Fluor™ 647

产品规格盖玻片

环保功能危害更小

数量1 kit

运输条件室温

颜色绿色、远红色、红色

发射可见光

适用于（设备）Floid™ 细胞成像系统, 荧光显微镜, 荧光成像仪

产品线Alexa Fluor™, Click-iT™

产品类型成像试剂盒

Unit SizeEach

内容与储存

在冰箱（2°C 至 8°C）中储存。

常见问题解答 (FAQ)

What are the main characteristics of a Click-iT reaction?

Click reactions have several general characteristics: the reaction is efficient, no extreme temperatures or solvents are required, the reaction is complete within 30 minutes, the components of the reaction are bio-inert, and perhaps most importantly, no side reactions occur-the label and detection tags react selectively and specifically with one another. This final point is a key advantage of this powerful detection technique; it is possible to apply click chemistry-labeled molecules to complex biological samples and detect them with unprecedented sensitivity due to the extremely low background of the reaction.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I will be performing a cell proliferation assay using Click-iT EdU kit. At what point can I stop overnight, or do I have to perform all the steps continuously?

One may store the sample after fixation overnight in PBS at 4^oC. For longer storage (<1 week) , store in buffer with 1-2% formaldehyde or in formalin to limit microbial growth. If you use sodium azide as a microbial inhibitor, it must be completely removed prior to the Click-iT reaction.

Find additional tips, troubleshooting help, and resources within our Cell Viability, Proliferation, Cryopreservation, and Apoptosis Support Center.

A control for a Click-iT EdU labeling experiment uses no EdU and the Click-iT reaction using Alexa Fluor 594 azide. The mouse heart tissue sections are showing non-specific labeling in red, seen in particular clusters of cells. They don't overlap with DAPI. What is the problem?

The problem is likely not the Alexa Fluor 594 azide. Since there are no alkynes endogenous to mouse tissue, there is nothing for the dye-azide to bind to. Since the background doesn't overlap with nuclei (DAPI signal), this isn't an issue of unintended EdU labeling. This red is autofluorescence from red blood cells; they autofluoresce in the red and don't have nuclei. This can be confirmed by checking a completely unlabeled tissue section (no dye present at all) to see if they are still present and by examining the cells at high magnification and looking for corpuscular shape.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I combine Click-iT or Click-iT Plus reactions with phalloidin conjugates used for actin staining?

We do not recommend using phalloidin conjugates for staining actin in combination with traditional Click-iT or Click-iT Plus reactions since phalloidin is extremely sensitive to the presence of copper.

For staining actin in combination with traditional Click-iT or Click-iT Plus reactions, we recommend using anti-α-actin antibodies for staining actin in the cytoskeleton. You can find a list of our actin antibodies here.

Another option would be to use the Click-iT Plus Alexa Fluor Picolyl Azide Toolkit (Cat. Nos. C10641, C10642, C10643). These Click-iT Plus toolkits provide Copper and Copper protectant separately which makes it easier to titrate the copper concentration to obtain optimal labeling with minimal copper-mediated damage. You may need to optimize the click reaction with the lowest possible concentration of copper and then perform the phalloidin staining.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Are the Alexa Fluor azides from Click-iT EdU kits available separately?

Yes, but the standalone products are not shipped at the same amount as provided in the Click-iT EdU kits; the amount of dye-azide provided in the Click-iT kits is proprietary information. See these catalog numbers for the standalone products:
- Cat. No. A10266: Alexa Fluor 488 azide
- Cat. No. A10270: Alexa Fluor 594 azide
- Cat. No. A10277: Alexa Fluor 647 azide

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.