Novel image cytometric method for detection of physiological and metabolic changes in Saccharomyces cerevisiae.
AuthorsChan LL, Kury A, Wilkinson A, Berkes C, Pirani A,
JournalJ Ind Microbiol Biotechnol
PubMed ID22864608
The studying and monitoring of physiological and metabolic changes in Saccharomyces cerevisiae (S. cerevisiae) has been a key research area for the brewing, baking, and biofuels industries, which rely on these economically important yeasts to produce their products. Specifically for breweries, physiological and metabolic parameters such as viability, vitality, glycogen, ... More
Neutropenia enhances lung dendritic cell recruitment in response to Aspergillus via a cytokine-to-chemokine amplification loop.
Current understanding of specific defense mechanisms in the context of neutropenic infections is limited. It has previously been reported that invasive aspergillosis, a prototypic opportunistic infection in neutropenic hosts, is associated with marked accumulation of inflammatory dendritic cells (DCs) in the lungs. Given recent data indicating that neutrophils can modulate ... More
Lack of glucose recycling between endoplasmic reticulum and cytoplasm underlies cellular dysfunction in glucose-6-phosphatase-beta-deficient neutrophils in a congenital neutropenia syndrome.
AuthorsJun HS, Lee YM, Cheung YY, McDermott DH, Murphy PM, De Ravin SS, Mansfield BC, Chou JY
JournalOthers
PubMed ID20498302
'G6PC3 deficiency, characterized by neutropenia and neutrophil dysfunction, is caused by deficiencies in the endoplasmic reticulum (ER) enzyme glucose-6-phosphatase-beta (G6Pase-beta or G6PC3) that converts glucose-6-phosphate (G6P) into glucose, the primary energy source of neutrophils. Enhanced neutrophil ER stress and apoptosis underlie neutropenia in G6PC3 deficiency, but the exact functional role ... More
Enumeration of human antigen-specific naive CD8+ T cells reveals conserved precursor frequencies.
AuthorsAlanio C, Lemaitre F, Law HK, Hasan M, Albert ML,
JournalBlood
PubMed ID20200354
'The number of antigen-specific naive CD8(+) T cells is believed to be important in the shaping of adaptive immune responses, and is predictive for the magnitude of priming responses in mouse models. Because of extremely low precursor frequencies, knowledge about these cells comes from indirect techniques and estimations. Here, we ... More
'Abstract Adeno-associated viral (AAV) vectors preferentially integrate into the genome of cells that are defective in DNA repair, such as occurs with DNA-PKcs deficiency or poly(ADP-ribose) polymerase-1 down-regulation. As the tumor suppressor protein p53 regulates the transcription of many genes involved in DNA repair, we sought to determine whether functional ... More
A transcribed pseudogene of MYLK promotes cell proliferation.
AuthorsHan YJ, Ma SF, Yourek G, Park YD, Garcia JG,
JournalFASEB J
PubMed ID21441351
'Pseudogenes are considered nonfunctional genomic artifacts of catastrophic pathways. Recent evidence, however, indicates novel roles for pseudogenes as regulators of gene expression. We tested the functionality of myosin light chain kinase pseudogene (MYLKP1) in human cells and tissues by RT-PCR, promoter activity, and cell proliferation assays. MYLKP1 is partially duplicated ... More
An integrated genomic analysis of aryl hydrocarbon receptor-mediated inhibition of B-cell differentiation.
AuthorsDe Abrew KN, Kaminski NE, Thomas RS,
JournalToxicol Sci
PubMed ID20819909
'The aryl hydrocarbon receptor (AHR) agonist 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) alters differentiation of B cells and suppresses antibody production. A combination of whole-genome, microarray-based chromatin immunoprecipitation (ChIP-on-chip), and time course gene expression microarray analysis was performed on the mouse B-cell line CH12.LX following exposure to lipopolysaccharide (LPS) or LPS and TCDD to ... More
Proliferation and differentiation potential of CD133+ and CD34+ populations from the bone marrow and mobilized peripheral blood.
AuthorsKoutna I, Peterkova M, Simara P, Stejskal S, Tesarova L, Kozubek M,
JournalAnn Hematol
PubMed ID20821012
'CD34 is the most frequently used marker for the selection of cells for bone marrow (BM) transplantation. The use of CD133 as an alternative marker is an open research topic. The goal of this study was to evaluate the proliferation and differentiation potential for hematopoiesis (short and long term) of ... More
In vitro growth of microsporidia Anncaliia algerae in cell lines from warm water fish.
AuthorsMonaghan SR, Rumney RL, Vo NT, Bols NC, Lee LE,
JournalIn Vitro Cell Dev Biol Anim
PubMed ID21086187
'Anncaliia algerae is an aquatic microsporidium that most commonly infects mosquitoes but can be grown on the rabbit kidney cell line, RK-13. Spores were purified from RK-13 cultures and added to cell lines from warm water fish and from an insect. The cell lines were GFSK-S1 and GFB3C-W1 from goldfish ... More
Hydralazine modifies Aß fibril formation and prevents modification by lipids in vitro.
AuthorsMaheshwari M, Roberts JK, Desutter B, Duong KT, Tingling J, Fawver JN, Schall HE, Kahle M, Murray IV,
JournalBiochemistry
PubMed ID21058733
'Lipid oxidative damage and amyloid ß (Aß) misfolding contribute to Alzheimer''s disease (AD) pathology. Thus, the prevention of oxidative damage and Aß misfolding are attractive targets for drug discovery. At present, no AD drugs approved by the Food and Drug Administration (FDA) prevent or halt disease progression. Hydralazine, a smooth ... More
A novel translocation breakpoint within the BPTF gene is associated with a pre-malignant phenotype.
AuthorsBuganim Y, Goldstein I, Lipson D, Milyavsky M, Polak-Charcon S, Mardoukh C, Solomon H, Kalo E, Madar S, Brosh R, Perelman M, Navon R, Goldfinger N, Barshack I, Yakhini Z, Rotter V,
JournalPLoS One
PubMed ID20300178
'Partial gain of chromosome arm 17q is an abundant aberrancy in various cancer types such as lung and prostate cancer with a prominent occurrence and prognostic significance in neuroblastoma--one of the most common embryonic tumors. The specific genetic element/s in 17q responsible for the cancer-promoting effect of these aberrancies is ... More
A novel transporter of SLC22 family specifically transports prostaglandins and co-localizes with 15-hydroxyprostaglandin dehydrogenase in renal proximal tubules.
AuthorsShiraya K, Hirata T, Hatano R, Nagamori S, Wiriyasermkul P, Jutabha P, Matsubara M, Muto S, Tanaka H, Asano S, Anzai N, Endou H, Yamada A, Sakurai H, Kanai Y,
JournalJ Biol Chem
PubMed ID20448048
'We identified a novel prostaglandin (PG)-specific organic anion transporter (OAT) in the OAT group of the SLC22 family. The transporter designated OAT-PG from mouse kidney exhibited Na(+)-independent and saturable transport of PGE(2) when expressed in a proximal tubule cell line (S(2)). Unusual for OAT members, OAT-PG showed narrow substrate selectivity ... More
Betacellulin inhibits osteogenic differentiation and stimulates proliferation through HIF-1alpha.
AuthorsGenetos DC, Rao RR, Vidal MA,
JournalCell Tissue Res
PubMed ID20165885
'Cellular signaling via epidermal growth factor (EGF) and EGF-like ligands can determine cell fate and behavior. Osteoblasts, which are responsible for forming and mineralizing osteoid, express EGF receptors and alter rates of proliferation and differentiation in response to EGF receptor activation. Transgenic mice over-expressing the EGF-like ligand betacellulin (BTC) exhibit ... More
Dissection of RAS downstream pathways in melanomagenesis: a role for Ral in transformation.
AuthorsMishra PJ, Ha L, Rieker J, Sviderskaya EV, Bennett DC, Oberst MD, Kelly K, Merlino G,
JournalOncogene
PubMed ID20118982
'Cutaneous malignant melanoma is considered one of the most deadly human cancers, based on both its penchant for metastatic spread and its typical resistance to currently available therapy. Long known to harbor oncogenic NRAS mutations, melanomas were more recently reported to be frequent bearers of activating mutations in BRAF, one ... More
The epidermal growth factor receptor mediates tumor necrosis factor-alpha-induced activation of the ERK/GEF-H1/RhoA pathway in tubular epithelium.
AuthorsKakiashvili E, Dan Q, Vandermeer M, Zhang Y, Waheed F, Pham M, Szászi K,
JournalJ Biol Chem
PubMed ID21212278
'Tumor necrosis factor (TNF)-a induces cytoskeleton and intercellular junction remodeling in tubular epithelial cells; the underlying mechanisms, however, are incompletely explored. We have previously shown that ERK-mediated stimulation of the RhoA GDP/GTP exchange factor GEF-H1/Lfc is critical for TNF-a-induced RhoA stimulation. Here we investigated the upstream mechanisms of ERK/GEF-H1 activation. ... More
Effects of ionizing radiation on self-renewal and pluripotency of human embryonic stem cells.
AuthorsWilson KD, Sun N, Huang M, Zhang WY, Lee AS, Li Z, Wang SX, Wu JC,
JournalCancer Res
PubMed ID20530673
'Human embryonic stem cells (hESC) present a novel platform for in vitro investigation of the early embryonic cellular response to ionizing radiation. Thus far, no study has analyzed the genome-wide transcriptional response to ionizing radiation in hESCs, nor has any study assessed their ability to form teratomas, the definitive test ... More
Teriflunomide (leflunomide) promotes cytostatic, antioxidant, and apoptotic effects in transformed prostate epithelial cells: evidence supporting a role for teriflunomide in prostate cancer chemoprevention.
AuthorsHail N, Chen P, Bushman LR,
JournalNeoplasia
PubMed ID20563249
'Teriflunomide (TFN) is an inhibitor of de novo pyrimidine synthesis and the active metabolite of leflunomide. Leflunomide is prescribed to patients worldwide as an immunomodulatory and anti-inflammatory disease-modifying prodrug. Leflunomide inhibited the growth of human prostate cancer xenographs in mice, and leflunomide or TFN promoted cytostasis and/or apoptosis in cultured ... More
Pressure driven spinning: A multifaceted approach for preparing nanoscaled functionalized fibers, scaffolds, and membranes with advanced materials.
AuthorsJayasinghe SN, Suter N,
JournalBiomicrofluidics
PubMed ID20644675
'Electrospinning, a flexible jet-based fiber, scaffold, and membrane fabrication approach, has been elucidated as having significance to the heath sciences. Its capabilities have been most impressive as it possesses the ability to spin composite fibers ranging from the nanometer to the micrometer scale. Nonetheless, electrospinning has limitations and hazards, negating ... More
Feed-forward signaling by membrane-bound ligand receptor circuit: the case of NOTCH DELTA-like 4 ligand in endothelial cells.
AuthorsCaolo V, van den Akker NM, Verbruggen S, Donners MM, Swennen G, Schulten H, Waltenberger J, Post MJ, Molin DG,
JournalJ Biol Chem
PubMed ID20959466
'The DELTA like-4 ligand (DLL4) belongs to the highly conserved NOTCH family and is specifically expressed in the endothelium. DLL4 regulates crucial processes in vascular growth, including endothelial cell (EC) sprouting and arterial specification. Its expression is increased by VEGF-A. In the present study, we show that VEGF-induced DLL4 expression ... More
Circadian clocks in human red blood cells.
AuthorsO'Neill JS, Reddy AB,
JournalNature
PubMed ID21270888
'Circadian (~24 hour) clocks are fundamentally important for coordinated physiology in organisms as diverse as cyanobacteria and humans. All current models of the molecular circadian clockwork in eukaryotic cells are based on transcription-translation feedback loops. Non-transcriptional mechanisms in the clockwork have been difficult to study in mammalian systems. We circumvented ... More
Development of a cellular assay system to study the genome replication of high- and low-risk mucosal and cutaneous human papillomaviruses.
AuthorsGeimanen J, Isok-Paas H, Pipitch R, Salk K, Laos T, Orav M, Reinson T, Ustav M, Ustav M, Ustav E,
JournalJ Virol
PubMed ID21248030
'We found that recircularized high-risk (type 16 and 18) and low-risk mucosal (type 6b and 11) and cutaneous (type 5 and 8) human papillomavirus (HPV) genomes replicate readily when delivered into U2OS cells by electroporation. The replication efficiency is dependent on the amount of input HPV DNA and can be ... More
Role of caspases and CD95/Fas in the apoptotic effects of a nucleotide analog PMEG in CCRF-CEM cells.
AuthorsMertlíková-Kaiserová H, Votruba I, Matousová M, Holy A, Hájek M,
JournalAnticancer Res
PubMed ID20683014
'9-[2-(phosphonomethoxy)ethyl] guanine (PMEG) is a guanine acyclic nucleotide analog whose targeted prodrugs are being investigated for chemotherapy of lymphomas. Its antiproliferative effects have been attributed to cell cycle arrest and induction of apoptosis, however, the underlying mechanisms remain poorly understood. The objective of this study was to determine the requirements ... More
Potential role for vascular endothelial growth factor-D as an autocrine factor for human gastric carcinoma cells.
AuthorsTanaka M, Kitadai Y, Kodama M, Shinagawa K, Sumida T, Tanaka S, Oue N, Yasui W, Chayama K,
JournalCancer Sci
PubMed ID20626397
'Vascular endothelial growth factor (VEGF)-D induces lymphangiogenesis by activating VEGF receptor (VEGFR)-3, which is expressed mainly by lymphatic endothelial cells. VEGFR-3 has also been detected in several types of malignant cells, but the significance of VEGFR-3 expression by malignant cells remains unclear. We examined the expression and function of VEGF-D/VEGFR-3 ... More
Heterochromatin formation in bistable chromatin domains controls the epigenetic repression of clonally variant Plasmodium falciparum genes linked to erythrocyte invasion.
AuthorsCrowley VM, Rovira-Graells N, de Pouplana LR, Cortés A,
JournalMol Microbiol
PubMed ID21306446
'Clonally variant gene expression is a common survival strategy used by many pathogens, including the malaria parasite Plasmodium falciparum. Among the genes that show variant expression in this parasite are several members of small gene families linked to erythrocyte invasion, including the clag and eba families. The active or repressed ... More
Src family kinases are necessary for cell migration induced by extracellular HMGB1.
AuthorsPalumbo R, De Marchis F, Pusterla T, Conti A, Alessio M, Bianchi ME,
JournalJ Leukoc Biol
PubMed ID19401391
HMGB1 is a nuclear protein that signals tissue damage, as it is released by cells dying traumatically or secreted by activated innate immunity cells. Extracellular HMGB1 elicits the migration to the site of tissue damage of several cell types, including inflammatory cells and stem cells. The identity of the signaling ... More
Knockdown of DAPIT (diabetes-associated protein in insulin-sensitive tissue) results in loss of ATP synthase in mitochondria.
AuthorsOhsakaya S, Fujikawa M, Hisabori T, Yoshida M,
JournalJ Biol Chem
PubMed ID21345788
It was found recently that a diabetes-associated protein in insulin-sensitive tissue (DAPIT) is associated with mitochondrial ATP synthase. Here, we report that the suppressed expression of DAPIT in DAPIT-knockdown HeLa cells causes loss of the population of ATP synthase in mitochondria. Consequently, DAPIT-knockdown cells show smaller mitochondrial ATP synthesis activity, ... More
p38 MAPK and NF-kappaB pathways are involved in naphtho[1,2-b] furan-4,5-dione induced anti-proliferation and apoptosis of human hepatoma cells.
AuthorsChiu CC, Chen JY, Lin KL, Huang CJ, Lee JC, Chen BH, Chen WY, Lo YH, Chen YL, Tseng CH, Chen YL, Lin SR,
JournalCancer Lett
PubMed ID20350781
Naphtho[1,2-b] furan-4,5-dione (NFD) was investigated for its anti-proliferation effect on human hepatocellular carcinoma (HCC), Hep3B, HepG(2), and Huh-7 cells. The effect of NFD on inhibiting proliferation and apoptosis was correlated with up-regulation of pro-apoptotic protein and down-regulation of pro-survival proteins. Remarkably, we found that NFD inhibited the nuclear translocation of ... More
The kinase inhibitor TKI258 is active against the novel CUX1-FGFR1 fusion detected in a patient with T-lymphoblastic leukemia/lymphoma and t(7;8)(q22;p11).
AuthorsWasag B, Lierman E, Meeus P, Cools J, Vandenberghe P,
JournalHaematologica
PubMed ID21330321
We report a patient with T-lymphoblastic leukemia/lymphoma and a t(7;8)(q22;p11). CUX1 was identified as the fusion partner of FGFR1 by fluorescence in situ hybridization and 5' RACE-PCR. We further investigated this novel FGFR1 fusion using the interleukin-3 (IL-3) dependent Ba/F3 cell line and demonstrated IL-3 independent cell growth of CUX1-FGFR1 ... More
Leptin inhibits hepatocellular carcinoma proliferation via p38-MAPK-dependent signalling.
AuthorsThompson KJ, Lau KN, Johnson S, Martinie JB, Iannitti DA, McKillop IH, Sindram D,
JournalHPB (Oxford)
PubMed ID21418127
Obesity is a significant risk factor for many liver diseases, including hepatocellular carcinoma (HCC). Leptin has been identified as a central mediator of factors that regulate energy intake and expenditure, including appetite, metabolism and fat storage. The role of leptin in the initiation, development and progression of HCC remains poorly ... More
Mechanisms and functional consequences of PDEF protein expression loss during prostate cancer progression.
AuthorsTurner DP, Findlay VJ, Moussa O, Semenchenko VI, Watson PM, Larue AC, Desouki MM, Fraig M, Watson DK,
JournalProstate
PubMed ID21446014
BACKGROUND: Ets is a large family of transcriptional regulators with functions in most biological processes. While the Ets family gene, prostate-derived epithelial factor (PDEF), is expressed in epithelial tissues, PDEF protein expression has been found to be reduced or lost during cancer progression. The goal of this study was to ... More
Synthetic sulfonyl-hydrazone-1 positively regulates cardiomyogenic microRNA expression and cardiomyocyte differentiation of induced pluripotent stem cells.
AuthorsQuattrocelli M, Palazzolo G, Agnolin I, Martino S, Bouché M, Anastasia L, Sampaolesi M,
JournalJ Cell Biochem
PubMed ID21445862
Induced pluripotent stem cells (iPSCs) are obtained from adult cells through overexpression of pluripotency factors. iPSCs share many features with embryonic stem cells (ESCs), circumventing ethical issues, and, noteworthy, match donor's genotype. iPSCs represent therefore a valuable tool for regenerative medicine. Cardiac differentiation of ESCs can be enhanced via microRNAs ... More
Engineering adipose tissue from uncultured human adipose stromal vascular fraction on collagen matrix and gelatin sponge scaffolds.
AuthorsLin SD, Huang SH, Lin YN, Wu SH, Chang HW, Lin TM, Chai CY, Lai CS,
JournalTissue Eng Part A
PubMed ID21247363
Adipogenic potential was evaluated in uncultured stromal vascular fraction (SVF) loaded onto porous 3D collagen matrix and gelatin sponge scaffolds with predefined shapes. The SVF was isolated from 16 freshly lipectomized fat. Mean cell number was 6.0±4.68×10(7) cells/mL, and mean cell viability was 72%. Flow cytometric analysis revealed adipose-derived stromal ... More
A novel synthetic protoapigenone analogue, WYC02-9, induces DNA damage and apoptosis in DU145 prostate cancer cells through generation of reactive oxygen species.
The protoapigenone analogue WYC02-9, a novel synthetic flavonoid, has been shown to act against a variety of experimental tumors. However, its effects on prostate cancer and its mechanism of action are unknown. Thus, WYC02-9 was investigated for its cytotoxicity against DU145 prostate cancer cells, as was the underlying mechanisms by ... More
Loss of the Birt-Hogg-Dubé tumor suppressor results in apoptotic resistance due to aberrant TGFß-mediated transcription.
AuthorsCash TP, Gruber JJ, Hartman TR, Henske EP, Simon MC,
JournalOncogene
PubMed ID21258407
Birt-Hogg-Dubé (BHD) syndrome is an inherited cancer susceptibility disease characterized by skin and kidney tumors, as well as cystic lung disease, which results from loss-of-function mutations in the BHD gene. BHD is also inactivated in a significant fraction of patients with sporadic renal cancers and idiopathic cystic lung disease, and ... More
Regulation of Bach2 by the aryl hydrocarbon receptor as a mechanism for suppression of B-cell differentiation by 2,3,7,8-tetrachlorodibenzo-p-dioxin.
Exposure to the aryl hydrocarbon receptor (AHR) agonist, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) alters B-cell differentiation and suppresses antibody production. Previous genomic studies in mouse B cells identified Bach2 as a direct target of the AHR. Bach2 is known to repress expression of Prdm1, a key transcription factor involved in B-cell differentiation, by ... More
Comparative proteomic analysis of blood eosinophils reveals redox signaling modifications in patients with FIP1L1-PDGFRA-associated chronic eosinophilic leukemia.
AuthorsKahn JE, Dutoit-Lefevre V, Duban-Deweer S, Chafey P, Pottiez G, Lefranc D, Fain O, Cordier JF, Hatron PY, Bletry O, Prin L,
JournalJ Proteome Res
PubMed ID21302907
The FIP1L1-PDGFRA (F/P) fusion gene, which was identified as a recurrent molecular finding in hypereosinophilic syndrome (HES), lead to a constitutively increased tyrosine kinase activity of the fusion protein. Despite data obtained in animals or cell lines models, the mechanisms underlying the predominant eosinophil lineage targeting and the cytotoxicity of ... More
Hydrogen peroxide probes directed to different cellular compartments.
AuthorsMalinouski M, Zhou Y, Belousov VV, Hatfield DL, Gladyshev VN,
JournalPLoS One
PubMed ID21283738
Controlled generation and removal of hydrogen peroxide play important roles in cellular redox homeostasis and signaling. We used a hydrogen peroxide biosensor HyPer, targeted to different compartments, to examine these processes in mammalian cells. Reversible responses were observed to various redox perturbations and signaling events. HyPer expressed in HEK 293 ... More
Human dermal fibroblasts derived from oculodentodigital dysplasia patients suggest that patients may have wound-healing defects.
AuthorsChurko JM, Shao Q, Gong XQ, Swoboda KJ, Bai D, Sampson J, Laird DW,
JournalHum Mutat
PubMed ID21305658
Oculodentodigital dysplasia (ODDD) is primarily an autosomal dominant human disease caused by any one of over 60 mutations in the GJA1 gene encoding the gap junction protein Cx43. In the present study, wound healing was investigated in a G60S ODDD mutant mouse model and by using dermal fibroblasts isolated from ... More
A pairwise chemical genetic screen identifies new inhibitors of glucose transport.
AuthorsUlanovskaya OA, Cui J, Kron SJ, Kozmin SA,
JournalChem Biol
PubMed ID21338919
Oxidative phosphorylation (OXPHOS) and glycolysis are the two main pathways that control energy metabolism of a cell. The Warburg effect, in which glycolysis remains active even under aerobic conditions, is considered a key driver for cancer cell proliferation, malignancy, metastasis, and therapeutic resistance. To target aerobic glycolysis, we exploited the ... More
Cell derived liposomes expressing CCR5 as a new targeted drug-delivery system for HIV infected cells.
AuthorsBronshtein T, Toledano N, Danino D, Pollack S, Machluf M,
JournalJ Control Release
PubMed ID21362450
Anti-retroviral-therapies against HIV/AIDS focus on inhibiting viral growth and may slow AIDS progression, but not cure the disease. Here we describe an approach to treat HIV as a cellular pathology by targeting cell derived liposomes against HIV-infected cells. Cell-derived-liposomes were prepared from the cytoplasmatic membranes of cells expressing CCR5, the ... More
Label-free determination of the number of biomolecules attached to cells by measurement of the cell's electrophoretic mobility in a microchannel.
AuthorsAki A, Nair BG, Morimoto H, Kumar DS, Maekawa T,
JournalPLoS One
PubMed ID21206908
We developed a label-free method for a determination of the number of biomolecules attached to individual cells by measuring the electrophoretic mobility of the cells in a microchannel. The surface of a biological cell, which is dispersed in aqueous solution, is normally electrically charged and the charge quantity at the ... More
Nonsense mediated decay resistant mutations are a source of expressed mutant proteins in colon cancer cell lines with microsatellite instability.
Frameshift mutations in microsatellite instability high (MSI-High) colorectal cancers are a potential source of targetable neo-antigens. Many nonsense transcripts are subject to rapid degradation due to nonsense-mediated decay (NMD), but nonsense transcripts with a cMS in the last exon or near the last exon-exon junction have intrinsic resistance to nonsense-mediated ... More
A porcine model of acute lung injury by instillation of gastric fluid.
AuthorsMeers CM, De Wever W, Verbeken E, Mertens V, Wauters S, De Vleeschauwer SI, Vos R, Vanaudenaerde BM, Verleden GM, Van Raemdonck DE,
JournalJ Surg Res
PubMed ID21109258
About 15% of donor lungs are declined because of aspiration contributing to current organ shortage. The aim was to develop a porcine lung injury model by gastric juice (GJ) instillation to study different pretransplant treatment strategies. Pigs (n = 6/group) were anesthetized and monitored. At T0 bronchoalveolar lavage (BAL) was ... More
The hydroxyl functional group of N-(4-hydroxyphenyl)retinamide mediates cellular uptake and cytotoxicity in premalignant and malignant human epithelial cells.
AuthorsHail N, Chen P, Wempe MF,
JournalFree Radic Biol Med
PubMed ID20923701
In a previous study, we demonstrated that the anticancer synthetic retinoid N-(4-hydroxyphenyl)retinamide (4HPR) redox cycles at the mitochondrial enzyme dihydroorotate dehydrogenase to trigger anomalous reactive oxygen species (ROS) production and attendant apoptosis in transformed human epithelial cells. Furthermore, we speculated that the hydroxyl functional group of 4HPR was required for ... More
Functional characterization of Wilms tumor-suppressor WTX and tumor-associated mutants.
AuthorsKim MK, Min DJ, Rabin M, Licht JD,
JournalOncogene
PubMed ID20956941
The WTX, Wilms tumor-associated tumor-suppressor gene, is present on the X chromosome and a single WTX mutation may be sufficient to promote carcinogenesis. Unlike the WT1 tumor suppressor, a transcription factor, WTX lacks conserved functional protein domains. To study the function of WTX, we constructed inducible cell lines expressing WTX ... More
Aptamer conjugated magnetic nanoparticles as nanosurgeons.
AuthorsNair BG, Nagaoka Y, Morimoto H, Yoshida Y, Maekawa T, Kumar DS,
JournalNanotechnology
PubMed ID20947949
Magnetic nanoparticles have shown promise in the fields of targeted drug delivery, hyperthermia and magnetic resonance imaging (MRI) in cancer therapy. The ability of magnetic nanoparticles to undergo surface modification and the effect of external magnetic field in the dynamics of their movement make them an excellent nanoplatform for cancer ... More
Increased caspase activation and decreased TDP-43 solubility in progranulin knockout cortical cultures.
Null mutations in progranulin (GRN) are associated with frontotemporal lobar degeneration characterized by intraneuronal accumulation of TAR DNA-binding protein-43 (TDP-43). However, the mechanism by which GRN deficiency leads to neurodegeneration remains largely unknown. In primary cortical neurons derived from Grn knockout (Grn(-/-) ) mice, we found that Grn-deficiency causes significantly ... More
The infection of mammalian and insect cells with SFV bearing nsP1 palmitoylation mutations.
AuthorsKaro-Astover L, Sarova O, Merits A, Zusinaite E,
JournalVirus Res
PubMed ID20801176
Semliki Forest virus (SFV), an alphavirus, replicates in vertebrate host and mosquito vector cells. The virus-specific part of the replicase complex constitutes nonstructural proteins 1-4 (nsP1-nsP4) and is bound to cytoplasmic membranes by an amphipathic helix inside of nsP1 and through the palmitoylation of cysteine residues in nsP1. In mammalian ... More
A Mycobacterium ulcerans toxin, mycolactone, induces apoptosis in primary human keratinocytes and in HaCaT cells.
AuthorsBozzo C, Tiberio R, Graziola F, Pertusi G, Valente G, Colombo E, Small PL, Leigheb G,
JournalMicrobes Infect
PubMed ID20800104
The pathogenicity of Mycobacterium ulcerans (Buruli ulcer) depends on cytotoxic effect of its exotoxin mycolactone. Since epidermis represents a barrier against infectious agents and balanced apoptosis is essential in epidermal homeostasis, we explored if mycolactone A/B induces apoptosis on two human keratinocyte populations, stem cells (KSC) and transit amplifying cells ... More
Klf4 overexpression activates epithelial cytokines and inflammation-mediated esophageal squamous cell cancer in mice.
AuthorsTetreault MP, Wang ML, Yang Y, Travis J, Yu QC, Klein-Szanto AJ, Katz JP,
JournalGastroenterology
PubMed ID20816834
Esophageal squamous cell cancer accounts for more than 90% of cases of esophageal cancers. Its pathogenesis involves chronic epithelial irritation, although the factors involved in the inflammatory process and the mechanisms of carcinogenesis are unknown. We sought to develop a mouse model of this cancer. We used the ED-L2 promoter ... More
Survival of retinal ganglion cells in slice culture provides a rapid screen for olfactory ensheathing cell preparations.
AuthorsDai C, Qin Yin Z, Li Y, Raisman G, Li D,
JournalBrain Res
PubMed ID20682293
Transplants of olfactory ensheathing cells (OECs) cultured from the olfactory bulb are able to induce structural regeneration of severed central axons and return of function in rat models. For clinical purposes it would be preferable to obtain the cells from the more accessible olfactory mucosa in the nasal lining. However, ... More
Additive effects of C(2)-ceramide on paclitaxel-induced premature senescence of human lung cancer cells.
AuthorsChen JY, Hwang CC, Chen WY, Lee JC, Fu TF, Fang K, Chu YC, Huang YL, Lin JC, Tsai WH, Chang HW, Chen BH, Chiu CC,
JournalLife Sci
PubMed ID20624405
the aims of the study are to investigate the additive effect of exogenous short-carbon chain phospholipids, C(2)-ceramide, on an anti-cancer drug paclitaxel (PTX)-induced senescence of human non-small cell lung cancer (NSCLC) cells deficient in functional p53 and p16, and to examine whether mitogen-activated protein kinase (MAPK) plays a role in ... More
Radiation-induced DNA damage and repair in human gammadelta and alphabeta T-lymphocytes analysed by the alkaline comet assay.
AuthorsLisowska H, Deperas-Kaminska M, Haghdoost S, Parmryd I, Wojcik A,
JournalGenome Integr
PubMed ID20678258
ABSTRACT: It has been shown by a number of authors that the radiosensitivity of peripheral blood mononuclear cells (PBMC) is higher in cancer patients compared to healthy donors, which is interpreted as a sign of genomic instability. PBMC are composed of different cell subpopulations which are differently radiosensitive and the ... More
Membrane transport and intracellular sequestration of novel thiosemicarbazone chelators for the treatment of cancer.
AuthorsMerlot AM, Pantarat N, Lovejoy DB, Kalinowski DS, Richardson DR,
JournalMol Pharmacol
PubMed ID20605952
Iron is a critical nutrient for DNA synthesis and cellular proliferation. Targeting iron in cancer cells using specific chelators is a potential new strategy for the development of novel anticancer agents. One such chelator, 2-benzoylpyridine 4-ethyl-3-thiosemicarbazone (Bp4eT), possesses potent and selective anticancer activity (J Med Chem 50:3716-3729, 2007). To elucidate ... More
CHD7 functions in the nucleolus as a positive regulator of ribosomal RNA biogenesis.
AuthorsZentner GE, Hurd EA, Schnetz MP, Handoko L, Wang C, Wang Z, Wei C, Tesar PJ, Hatzoglou M, Martin DM, Scacheri PC,
JournalHum Mol Genet
PubMed ID20591827
De novo mutation of the gene encoding chromodomain helicase DNA-binding protein 7 (CHD7) is the primary cause of CHARGE syndrome, a complex developmental disorder characterized by the co-occurrence of a specific set of birth defects. Recent studies indicate that CHD7 functions as a transcriptional regulator in the nucleoplasm. Here, we ... More
Involvement of Grb2 adaptor protein in nucleophosmin-anaplastic lymphoma kinase (NPM-ALK)-mediated signaling and anaplastic large cell lymphoma growth.
AuthorsRiera L, Lasorsa E, Ambrogio C, Surrenti N, Voena C, Chiarle R,
JournalJ Biol Chem
PubMed ID20554525
Most anaplastic large cell lymphomas (ALCL) express oncogenic fusion proteins derived from chromosomal translocations or inversions of the anaplastic lymphoma kinase (ALK) gene. Frequently ALCL carry the t(2;5) translocation, which fuses the ALK gene to the nucleophosmin (NPM1) gene. The transforming activity mediated by NPM-ALK fusion induces different pathways that ... More
The extracellular matrix glycoprotein elastin microfibril interface located protein 2: a dual role in the tumor microenvironment.
AuthorsMongiat M, Marastoni S, Ligresti G, Lorenzon E, Schiappacassi M, Perris R, Frustaci S, Colombatti A,
JournalNeoplasia
PubMed ID20360940
We have recently reported that elastin microfibril interface located protein 2 (EMILIN2), an extracellular matrix (ECM) glycoprotein, triggers cell death through a direct binding to death receptors. EMILIN2 thus influences cell viability through a mechanism that is unique for an ECM molecule. In the present work, we report an additional ... More
A domesticated transposon mediates the effects of a single-nucleotide polymorphism responsible for enhanced muscle growth.
AuthorsButter F, Kappei D, Buchholz F, Vermeulen M, Mann M,
JournalEMBO Rep
PubMed ID20134481
Single-nucleotide polymorphisms (SNPs) in the regulatory regions of the genome can have a profound impact on phenotype. The G3072A polymorphism in intron 3 of insulin-like growth factor 2 (IGF2) is implicated in higher muscle content and reduced fat in European pigs and is bound by a putative repressor. Here, we ... More
Crystal structure of the avian astrovirus capsid spike.
AuthorsDubois RM, Freiden P, Marvin S, Reddivari M, Heath RJ, White SW, Schultz-Cherry S,
JournalJ Virol
PubMed ID23658448
Astroviruses are small, nonenveloped, single-stranded RNA viruses that cause diarrhea in a wide variety of mammals and birds. On the surface of the viral capsid are globular spikes that are thought to be involved in attachment to host cells. To understand the basis of species specificity, we investigated the structure ... More
Automated quantification of budding Saccharomyces cerevisiae using a novel image cytometry method.
The measurements of concentration, viability, and budding percentages of Saccharomyces cerevisiae are performed on a routine basis in the brewing and biofuel industries. Generation of these parameters is of great importance in a manufacturing setting, where they can aid in the estimation of product quality, quantity, and fermentation time of ... More
Therapeutic targeting of TLR9 inhibits cell growth and induces apoptosis in neuroblastoma.
AuthorsBrignole C, Marimpietri D, Di Paolo D, Perri P, Morandi F, Pastorino F, Zorzoli A, Pagnan G, Loi M, Caffa I, Erminio G, Haupt R, Gambini C, Pistoia V, Ponzoni M,
JournalCancer Res
PubMed ID20935225
The Toll-like receptor 9 (TLR9) evolved to cope with pathogens, but it is expressed in a variety of tumors for reasons that are unclear. In this study, we report that neuroblastoma (NB) cells express functional TLR9. Liposome-complexed CpG oligonucleotides inhibited the proliferation of TLR9-expressing NB cells and induced caspase-dependent apoptotic ... More
MicroRNA-375 Is Downregulated in Gastric Carcinomas and Regulates Cell Survival by Targeting PDK1 and 14-3-3{zeta}.
AuthorsTsukamoto Y, Nakada C, Noguchi T, Tanigawa M, Nguyen LT, Uchida T, Hijiya N, Matsuura K, Fujioka T, Seto M, Moriyama M
JournalOthers
PubMed ID20215506
We investigated expression profiles of microRNA (miRNA) in gastric carcinomas by use of a miRNA microarray platform covering a total of 470 human miRNAs. We identified 39 differentially expressed miRNAs in gastric carcinoma, of which six were significantly downregulated and the other 33 were upregulated. We found that ... More
Epidermal growth factor receptor and mutant p53 expand an esophageal cellular subpopulation capable of epithelial-to-mesenchymal transition through ZEB transcription factors.
AuthorsOhashi S, Natsuizaka M, Wong GS, Michaylira CZ, Grugan KD, Stairs DB, Kalabis J, Vega ME, Kalman RA, Nakagawa M, Klein-Szanto AJ, Herlyn M, Diehl JA, Rustgi AK, Nakagawa H
JournalOthers
PubMed ID20424117
Transforming growth factor-beta (TGF-beta) is a potent inducer of epithelial to mesenchymal transition (EMT). However, it remains elusive about which molecular mechanisms determine the cellular capacity to undergo EMT in response to TGF-beta. We have found that both epidermal growth factor receptor (EGFR) overexpression and mutant p53 tumor suppressor genes ... More