Countess™ Cell Counting Chamber Slides with Trypan Blue Stain, disposable, 50 Slides, 2 x 1 mL vials - FAQs

Countess II和Countess II FL仪器预先校准过的。您不需要校准仪器。

在使用0.2%台盼蓝的条件下，基于染料的排斥性。台盼蓝被活细胞排斥，能进入死细胞，从而使死细胞胞内的蛋白质被强烈的染色。 

下列的细胞系和原代细胞已经通过测试：

细胞系：HEK 293, A431, CHO-M1, CHSE, COLO-205, COS-7, HeLa, HepG2, HL-60, J774(MMM), Jurkat, K-562, MCF-7, MRC-5, NIH/3T3, PC-12, SF-21, U266, 和U2OS 
原代细胞：人类脂肪组织来源干细胞, HASMCs, HPAECs, HPASMCs, HUVECs, C2C12, RBCs,和PMBCs
我们在持续测试更多细胞。

当一个实验工作者使用玻璃血球计数板和显微镜手动计数细胞时，单个实验的重复计数的变化率一般为10%或更多。

Countess II和Countess II FL仪器的用户观测到的重复计数的变化率通常应该<5%。

在明场模式下计数，Countess II和Countess II FL仪器自动默认设定1：1与台盼蓝稀释。在荧光模式下，没有这样默认设定。

Countess II仪器被设计用于读取浓度范围从1 x 10E4到1 x 10E7细胞/mL的细胞样本。

每个样品花费的时间为10秒。

Countess II和Countess II FL仪器能够检测到微粒/细胞的范围为4–60 μm。大小在7–60 μm范围的细胞可以在明场下计数和评估细胞活力。

Yes. The alternative options for Countess Cell Counting Chamber Slides (Cat. No. C10283) and for Countess 3 and Countess 3 FL Automated Cell Counter can be found using the link below:

Countess Cell Counting Chamber Slides (Cat. Nos. C10228, C10312, C10313, C10314, C10315)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

The Countess Cell Counting Chamber Slides (Cat. No. C10228, C10312, C10314, C10315) are compatible with all Countess Automated Cell Counter instruments.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center .

The Countess II and Countess II FL instruments come precalibrated. You do not have to calibrate the instruments.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

It is based on dye exclusion using 0.2% Trypan Blue dye. Trypan Blue is excluded by the live cells and enters the dead cells; this results in intense staining of the intracellular proteins.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

The following cell lines and primary cells have been tested:

- Cell lines: HEK 293, A431, CHO-M1, CHSE, COLO-205, COS-7, HeLa, HepG2, HL-60, J774(MMM), Jurkat, K-562, MCF-7, MRC-5, NIH/3T3, PC-12, SF-21, U266, and U2OS

- Primary cells: Human adipose tissue-derived stem cells, HASMCs, HPAECs, HPASMCs, HUVECs, C2C12, RBCs, and PMBCs

We are continually testing additional cell types.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

When an experienced worker manually counts cells using a glass hemocytometer together with a microscope, count-to-count variability of a single sample is commonly 10% or more. Users of Countess II and Countess II FL instruments should typically see <5% count-to-count variability.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

When counting in brightfield mode, the Countess II and Countess II FL instruments automatically assume a 1:1 dilution has been made with trypan blue. The same assumption is not made when in fluorescence mode.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

The Countess II instruments are designed to read samples with concentrations in the range of 1 x 10E4 cells/mL to 1 x 10E7 cells/mL.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

The time taken is ˜10 seconds per sample.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

The Countess II and Countess II FL instruments can detect particles/cells ranging from 4 - 60 µm. Cells ranging from 7 - 60 µm can be counted and assessed for viability with brightfield viewing.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.