Auxin-induced Rapid Degradation of Inhibitor of Caspase-activated DNase (ICAD) Induces Apoptotic DNA Fragmentation, Caspase Activation, and Cell Death: A CELL SUICIDE MODULE.
'Caspase-activated DNase (CAD) is a major apoptotic nuclease, responsible for DNA fragmentation and chromatin condensation during apoptosis. CAD is normally activated in apoptosis as a result of caspase cleavage of its inhibitory chaperone ICAD. Other aspects of CAD regulation are poorly understood. In particular, it has been unclear whether direct ... More
Oxidative and endoplasmic reticulum stresses mediate apoptosis induced by modified LDL in human retinal Müller cells.
AuthorsWu M, Yang S, Elliott MH, Fu D, Wilson K, Zhang J, Du M, Chen J, Lyons T,
JournalInvest Ophthalmol Vis Sci
PubMed ID22678501
'We previously showed that extravasated, modified LDL is implicated in pericyte loss in diabetic retinopathy (DR). Here, we investigate whether modified LDL induces apoptosis in retinal Müller glial cells. Cultured human retinal Müller cells (MIO-M1) were treated with highly oxidized glycated LDL (HOG-LDL, 200 mg protein/L) or native LDL (N-LDL, ... More
Varicella-zoster virus infection of differentiated human neural stem cells.
'Primary varicella-zoster virus (VZV) infection in humans produces varicella (chickenpox), after which the virus becomes latent in ganglionic neurons. Analysis of the physical state of viral nucleic acid and virus gene expression during latency requires postmortem acquisition of fresh human ganglia. To provide an additional way to study the VZV-host ... More
Expression of a naturally occurring angiotensin AT(1) receptor cleavage fragment elicits caspase-activation and apoptosis.
AuthorsCook JL, Singh A, DeHaro D, Alam J, Re RN,
JournalAm J Physiol Cell Physiol
PubMed ID21813711
'Several transmembrane receptors are documented to accumulate in nuclei, some as holoreceptors and others as cleaved receptor products. Our prior studies indicate that a population of the 7-transmembrane angiotensin type-1 receptor (AT(1)R) is cleaved in a ligand-augmented manner after which the cytoplasmic, carboxy-terminal cleavage fragment (CF) traffics to the nucleus. ... More
Astrocytes secrete exosomes enriched with proapoptotic ceramide and prostate apoptosis response 4 (PAR-4): potential mechanism of apoptosis induction in Alzheimer disease (AD).
AuthorsWang G, Dinkins M, He Q, Zhu G, Poirier C, Campbell A, Mayer-Proschel M, Bieberich E,
JournalJ Biol Chem
PubMed ID22532571
'Amyloid protein is well known to induce neuronal cell death, whereas only little is known about its effect on astrocytes. We found that amyloid peptides activated caspase 3 and induced apoptosis in primary cultured astrocytes, which was prevented by caspase 3 inhibition. Apoptosis was also prevented by shRNA-mediated down-regulation of ... More
Paper-supported 3D cell culture for tissue-based bioassays.
AuthorsDerda R, Laromaine A, Mammoto A, Tang SK, Mammoto T, Ingber DE, Whitesides GM,
JournalProc Natl Acad Sci U S A
PubMed ID19846768
Fundamental investigations of human biology, and the development of therapeutics, commonly rely on 2D cell-culture systems that do not accurately recapitulate the structure, function, or physiology of living tissues. Systems for 3D cultures exist but do not replicate the spatial distributions of oxygen, metabolites, and signaling molecules found in tissues. ... More
In vivo characterization of alveolar and interstitial lung macrophages in rhesus macaques: implications for understanding lung disease in humans.
AuthorsCai Y, Sugimoto C, Arainga M, Alvarez X, Didier ES, Kuroda MJ,
Journal
PubMed ID24534529
Alveolar macrophages (AMs) obtained by bronchoalveolar lavage (BAL) are commonly used to study lung macrophage-mediated immune responses. Questions remain, however, about whether AMs fully represent macrophage function in the lung. This study was performed to determine the contribution of interstitial macrophages (IMs) of lung tissue to pulmonary immunity and that ... More
Vasopressin inhibits apoptosis in renal collecting duct cells.
The peptide hormone arginine vasopressin (AVP) plays a critical role in regulating salt and water transport in the mammalian kidney. Recent studies have also demonstrated that AVP can promote cell survival in neuronal cells through V1 receptors. The current study addresses whether AVP can inhibit apoptosis in kidney collecting duct ... More
An in vivo model of functional and vascularized human brain organoids.
Authors
JournalNat Biotechnol
PubMed ID29658944
PARP1 inhibition alleviates injury in ARH3-deficient mice and human cells.
AuthorsMashimo M, Bu X, Aoyama K, Kato J, Ishiwata-Endo H, Stevens LA, Kasamatsu A, Wolfe LA, Toro C, Adams D, Markello T, Gahl WA, Moss J
JournalJCI Insight
PubMed ID30830864
'Poly(ADP-ribosyl)ation refers to the covalent attachment of ADP-ribose to protein, generating branched, long chains of ADP-ribose moieties, known as poly(ADP-ribose) (PAR). Poly(ADP-ribose) polymerase 1 (PARP1) is the main polymerase and acceptor of PAR in response to DNA damage. Excessive intracellular PAR accumulation due to PARP1 activation leads cell death in ... More
Identification of a conserved and acute neurodegeneration-specific microglial transcriptome in the zebrafish.
AuthorsOosterhof N, Holtman IR, Kuil LE, van der Linde HC, Boddeke EW, Eggen BJ, van Ham TJ
JournalGlia
PubMed ID27757989
'Microglia are brain resident macrophages important for brain development, connectivity, homeostasis and disease. However, it is still largely unclear how microglia functions and their identity are regulated at the molecular level. Although recent transcriptomic studies have identified genes specifically expressed in microglia, the function of most of these genes in ... More
Chondrogenic differentiation of human chondrocytes cultured in the absence of ascorbic acid.
AuthorsAsnaghi MA, Duhr R, Quasnichka H, Hollander AP, Kafienah W, Martin I, Wendt D
JournalJ Tissue Eng Regen Med
PubMed ID29726103
'Bioreactor systems will likely play a key role in establishing regulatory compliant and cost-effective production systems for manufacturing engineered tissue grafts for clinical applications. However, the automation of bioreactor systems could become considerably more complex and costly due to the requirements for additional storage and liquid handling technologies if unstable ... More
The Interleukin (IL)-1R1 pathway is a critical negative regulator of PyMT-mediated mammary tumorigenesis and pulmonary metastasis.
Authors
JournalOncoimmunology
PubMed ID28405519
Oocytes can efficiently repair DNA double-strand breaks to restore genetic integrity and protect offspring health.
Authors
JournalProc Natl Acad Sci U S A
PubMed ID32381741
Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes.
Authors
JournalNat Commun
PubMed ID31249304
LIM Kinase Inhibitor Pyr1 Reduces the Growth and Metastatic Load of Breast Cancers.
Authors
JournalCancer Res
PubMed ID27216191
MEIS-mediated suppression of human prostate cancer growth and metastasis through HOXB13-dependent regulation of proteoglycans.
Authors
JournalElife
PubMed ID32553107
KLF5 promotes breast cancer proliferation, migration and invasion in part by upregulating the transcription of TNFAIP2.
Authors
JournalOncogene
PubMed ID26189798
Human Pluripotent Stem Cell-Derived Neural Cells and Brain Organoids Reveal SARS-CoV-2 Neurotropism Predominates in Choroid Plexus Epithelium.
Authors
JournalCell Stem Cell
PubMed ID33010822
Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos.
Authors
JournalCell
PubMed ID30550787
Development of near-infrared photoactivable phthalocyanine-loaded nanoparticles to kill tumor cells: An improved tool for photodynamic therapy of solid cancers.
AuthorsDuchi S, Ramos-Romero S, Dozza B, Guerra-Rebollo M, Cattini L, Ballestri M, Dambruoso P, Guerrini A, Sotgiu G, Varchi G, Lucarelli E, Blanco J
JournalNanomedicine
PubMed ID27133189
Conventional photodynamic therapy has shown to be beneficial in the treatment of a variety of tumors. However, one of its major limitations is the inadequate penetration depth of visible light. In order to overcome this constraint, we developed 80nm poly-methylmethacrylate core-shell fluorescent nanoparticles (FNP) loaded with the photosensitizer tetrasulfonated aluminum ... More
Pur-alpha regulates cytoplasmic stress granule dynamics and ameliorates FUS toxicity.
AuthorsDaigle JG, Krishnamurthy K, Ramesh N, Casci I, Monaghan J, McAvoy K, Godfrey EW, Daniel DC, Johnson EM, Monahan Z, Shewmaker F, Pasinelli P, Pandey UB
JournalActa Neuropathol
PubMed ID26728149
Amyotrophic lateral sclerosis is characterized by progressive loss of motor neurons in the brain and spinal cord. Mutations in several genes, including FUS, TDP43, Matrin 3, hnRNPA2 and other RNA-binding proteins, have been linked to ALS pathology. Recently, Pur-alpha, a DNA/RNA-binding protein was found to bind to C9orf72 repeat expansions ... More
Vascular changes in tumors resistant to a vascular disrupting nanoparticle treatment.
AuthorsSharma S, Mann AP, Mölder T, Kotamraju VR, Mattrey R, Teesalu T, Ruoslahti E
JournalJ Control Release
PubMed ID29030222
Anti-angiogenic and vascular disrupting therapies rely on the dependence of tumors on new blood vessels to sustain tumor growth. We previously reported a potent vascular disrupting agent, a theranostic nanosystem consisting of a tumor vasculature-homing peptide (CGKRK) fused to a pro-apoptotic peptide [ ... More
Cytotoxic effect of TDZ on human cervical cancer cells.
AuthorsEnkhtaivan G, Kim DH, Pandurangan M
JournalJ Photochem Photobiol B
PubMed ID28668518
The present study investigates the anticancer activity of Thidiazuron (TDZ). Anticancer activity of TDZ was evaluated in cervical carcinoma cells (HeLa cells). Sulforhodamine-B (SRB) assay indicates that TDZ was about 100 times more toxic to the cancer cell than normal cells. TUNEL assay showed TDZ induced DNA damage in tumor ... More
PDGF Modulates BMP2-Induced Osteogenesis in Periosteal Progenitor Cells.
AuthorsWang X, Matthews BG, Yu J, Novak S, Grcevic D, Sanjay A, Kalajzic I
JournalJBMR Plus
PubMed ID31131345
BMPs are used in various clinical applications to promote bone formation. The limited success of the BMPs in clinical settings and supraphysiological doses required for their effects prompted us to evaluate the influence of other signaling molecules, specifically platelet-derived growth factor (PDGF) on BMP2-induced osteogenesis. Periosteal cells make a major ... More
Lutein derived from marigold (Tagetes erecta) petals triggers ROS generation and activates Bax and caspase-3 mediated apoptosis of human cervical carcinoma (HeLa) cells.
AuthorsGansukh E, Mya KK, Jung M, Keum YS, Kim DH, Saini RK
JournalFood Chem Toxicol
PubMed ID30826408
The present investigation was designed to determine molecular and cellular events involved in anticancer properties of lutein derived from marigold (Tagetes erecta) petals using Human cervical carcinoma (HeLa) cell lines. In vitro experiments demonstrated that lutein at concentration of 10?µM significantly inhibited proliferation of HeLa cells by up to 62.85% ... More
Spontaneous dormancy protects
AuthorsSánchez-Valdéz FJ, Padilla A, Wang W, Orr D, Tarleton RL
JournalElife
PubMed ID29578409
The ability of the Chagas disease agent
A genetically encodable cell-type-specific protein synthesis inhibitor.
AuthorsHeumüller M, Glock C, Rangaraju V, Biever A, Schuman EM
JournalNat Methods
PubMed ID31308551
Chemical inhibitors have revealed requirements for protein synthesis that drive cellular plasticity. We developed a genetically encodable protein synthesis inhibitor (gePSI) to achieve cell-type-specific temporal control of protein synthesis. Controlled expression of the gePSI in neurons or glia resulted in rapid, potent and reversible cell-autonomous inhibition of protein synthesis. Moreover, ... More
Comparative study of the antitumoral activity of phosphine-thiosemicarbazone gold(I) complexes obtained by different methodologies.
A series of phosphino-thiosemicarbazone gold(I) dinuclear complexes obtained by two different synthetic procedures have been prepared. All the compounds have been spectroscopically characterized including single crystal X ray diffraction analysis in some of cases. [Au ... More
Pyrrolylquinoxaline-2-One Derivative as a Potent Therapeutic Factor for Brain Trauma Rehabilitation.
AuthorsDutysheva EA, Mikeladze MA, Trestsova MA, Aksenov ND, Utepova IA, Mikhaylova ER, Suezov RV, Charushin VN, Chupakhin ON, Guzhova IV, Margulis BA, Lazarev VF
JournalPharmaceutics
PubMed ID32366047
Traumatic brain injury (TBI) often causes massive brain cell death accompanied by the accumulation of toxic factors in interstitial and cerebrospinal fluids. The persistence of the damaged brain area is not transient and may occur within days and weeks. Chaperone Hsp70 is known for its cytoprotective and antiapoptotic activity, and ... More