Dynamic monitoring of newly synthesized proteomes: up-regulation of myristoylated protein kinase A during butyric acid induced apoptosis.
AuthorsLiu K, Yang PY, Na Z, Yao SQ,
JournalAngew Chem Int Ed Engl
PubMed ID21678537
Doubly charged: A double metabolic incorporation approach capable of proteome-wide profiling of post-translational modification dynamics on newly synthesized proteins has been developed (see scheme; blue box: methionine surrogate, orange diamond: PTM probe). This strategy reveals for the first time that up-regulation of myristoylated PKA protein is necessary for the occurrence ... More
The chemical neurobiology of carbohydrates.
AuthorsMurrey HE, Hsieh-Wilson LC,
JournalChem Rev
PubMed ID18452339
The problems associated with oligosaccharide analysis have hindered efforts to understand the biology of oligosaccharides yet have given chemists a unique opportunity to develop new methods to overcome these challenges. The development of chemical tools for the analysis of glycan structure and function is essential to advance our understanding of ... More
Metabolic click-labeling with a fucose analog reveals pectin delivery, architecture, and dynamics in Arabidopsis cell walls.
AuthorsAnderson CT, Wallace IS, Somerville CR,
JournalProc Natl Acad Sci U S A
PubMed ID22232683
Polysaccharide-rich cell walls are a defining feature of plants that influence cell division and growth, but many details of cell-wall organization and dynamics are unknown because of a lack of suitable chemical probes. Metabolic labeling using sugar analogs compatible with click chemistry has the potential to provide new insights into ... More
Glycoproteomic probes for fluorescent imaging of fucosylated glycans in vivo.
AuthorsSawa M, Hsu TL, Itoh T, Sugiyama M, Hanson SR, Vogt PK, Wong CH
JournalProc Natl Acad Sci U S A
PubMed ID16895981
Glycomics is emerging as a new field for the biology of complex glycoproteins and glycoconjugates. The lack of versatile glycan-labeling methods has presented a major obstacle to visualizing at the cellular level and studying glycoconjugates. To address this issue, we developed a fluorescent labeling technique based on the Cu(I)-catalyzed [3 ... More
Alkynyl sugar analogs for the labeling and visualization of glycoconjugates in cells.
AuthorsHsu TL, Hanson SR, Kishikawa K, Wang SK, Sawa M, Wong CH
JournalProc Natl Acad Sci U S A
PubMed ID17296930
Developing tools for investigating the cellular activity of glycans will help to delineate the molecular basis for aberrant glycosylation in pathological processes such as cancer. Metabolic oligosaccharide engineering, which inserts sugar-reporting groups into cellular glycoconjugates, represents a powerful method for imaging the localization, trafficking, and dynamics of glycans and isolating ... More