Selective identification of newly synthesized proteins in mammalian cells using bioorthogonal noncanonical amino acid tagging (BONCAT).
AuthorsSchuman EM
JournalProceedings of the National Academy of Sciences of the United States of America
PubMed ID16769897
In both normal and pathological states, cells respond rapidly to environmental cues by synthesizing new proteins. The selective identification of a newly synthesized proteome has been hindered by the basic fact that all proteins, new and old, share the same pool of amino acids and thus are chemically indistinguishable. We ... More
In situ visualization and dynamics of newly synthesized proteins in rat hippocampal neurons.
AuthorsSchuman EM
JournalNature neuroscience
PubMed ID20543841
Protein translation has been implicated in different forms of synaptic plasticity, but direct in situ visualization of new proteins is limited to one or two proteins at a time. Here we describe a metabolic labeling approach based on incorporation of noncanonical amino acids into proteins followed by chemoselective fluorescence tagging ... More
Two-color labeling of temporally defined protein populations in mammalian cells.
AuthorsTirrell DA
JournalBioorganic & medicinal chemistry letters
PubMed ID18774715
The proteome undergoes complex changes in response to disease, drug treatment, and normal cellular signaling processes. Characterization of such changes requires methods for time-resolved protein identification and imaging. Here, we describe the application of two reactive methionine (Met) analogues, azidohomoalanine (Aha) and homopropargylglycine (Hpg), to label two protein populations in ... More
Fluorescence visualization of newly synthesized proteins in mammalian cells.
AuthorsTirrell DA
JournalAngewandte Chemie (International ed. in English)
PubMed ID17036290
Noncanonical amino acid tagging enables the selective fluorescent visualization of newly synthesized proteins in mammalian cells (see the picture). Susceptibility to tagging is determined by the spatial and temporal character of the protein synthesis, thus providing a complement to methods which identify relevant members of the proteome. ... More
Spatial coupling of mTOR and autophagy augments secretory phenotypes.
AuthorsNarita M., et al
JournalScience (New York, N.Y.)
PubMed ID21512002
Protein synthesis and autophagic degradation are regulated in an opposite manner by mammalian target of rapamycin (mTOR), whereas under certain conditions it would be beneficial if they occurred in unison to handle rapid protein turnover. We observed a distinct cellular compartment at the trans side of the Golgi apparatus, the ... More
Viroporin activity of the JC polyomavirus is regulated by interactions with the adaptor protein complex 3.
AuthorsSuzuki T, Orba Y, Makino Y, Okada Y, Sunden Y, Hasegawa H, Hall WW, Sawa H,
Journal
PubMed ID24167297
'Viroporins, which are encoded by a wide range of animal viruses, oligomerize in host cell membranes and form hydrophilic pores that can disrupt a number of physiological properties of the cell. Little is known about the relationship between host cell proteins and viroporin activity. The human JC polyomavirus (JCV) is ... More
Fasting cycles retard growth of tumors and sensitize a range of cancer cell types to chemotherapy.
AuthorsLee C, Raffaghello L, Brandhorst S, Safdie FM, Bianchi G, Martin-Montalvo A, Pistoia V, Wei M, Hwang S, Merlino A, Emionite L, de Cabo R, Longo VD,
JournalSci Transl Med
PubMed ID22323820
'Short-term starvation (or fasting) protects normal cells, mice, and potentially humans from the harmful side effects of a variety of chemotherapy drugs. Here, we show that treatment with starvation conditions sensitized yeast cells (Saccharomyces cerevisiae) expressing the oncogene-like RAS2(val19) to oxidative stress and 15 of 17 mammalian cancer cell lines ... More
Depletion of 4E-BP1 and regulation of autophagy lead to YXM110-induced anticancer effects.
AuthorsLai CY, Pan SL, Yang XM, Chang LH, Chang YL, Yang PC, Lee KH, Teng CM,
JournalCarcinogenesis
PubMed ID23633518
Natural products have always been a profuse database for developing new chemotherapeutics. YXM110 is a newly synthesized phenanthroquinolizidines that exhibits excellent anticancer activity in numerous cancer cells. In this study, we examined the anticancer mechanisms of YXM110 both in vitro and in vivo. Protein level of 4E-binding protein 1, which ... More
Genome-wide analysis of glucocorticoid receptor-binding sites in myotubes identifies gene networks modulating insulin signaling.
AuthorsKuo T, Lew MJ, Mayba O, Harris CA, Speed TP, Wang JC,
JournalProc Natl Acad Sci U S A
PubMed ID22733784
Glucocorticoids elicit a variety of biological responses in skeletal muscle, including inhibiting protein synthesis and insulin-stimulated glucose uptake and promoting proteolysis. Thus, excess or chronic glucocorticoid exposure leads to muscle atrophy and insulin resistance. Glucocorticoids propagate their signal mainly through glucocorticoid receptors (GR), which, upon binding to ligands, translocate to ... More
Development of a novel method for quantification of autophagic protein degradation by AHA labeling.
Authors
JournalAutophagy
PubMed ID24675368
The BAX-binding protein MOAP1 associates with LC3 and promotes closure of the phagophore.
Authors
JournalAutophagy
PubMed ID33783314
Synthetic Lethality of Wnt Pathway Activation and Asparaginase in Drug-Resistant Acute Leukemias.
Authors
JournalCancer Cell
PubMed ID30991026
Quantitative chemical proteomics profiling of de novo protein synthesis during starvation-mediated autophagy.
Authors
JournalAutophagy
PubMed ID27463841
Photodynamic therapy with redaporfin targets the endoplasmic reticulum and Golgi apparatus.
Authors
JournalEMBO J
PubMed ID29807932
Oleate-induced aggregation of LC3 at the trans-Golgi network is linked to a protein trafficking blockade.
Authors
JournalCell Death Differ
PubMed ID33335289
Importance of TFEB acetylation in control of its transcriptional activity and lysosomal function in response to histone deacetylase inhibitors.
Authors
JournalAutophagy
PubMed ID30059277
HuD Is a Neural Translation Enhancer Acting on mTORC1-Responsive Genes and Counteracted by the Y3 Small Non-coding RNA.
Authors
JournalMol Cell
PubMed ID30029004
eIF2α phosphorylation is pathognomonic for immunogenic cell death.
Authors
JournalCell Death Differ
PubMed ID29358668
Inhibition of transcription by dactinomycin reveals a new characteristic of immunogenic cell stress.