Click-iT™ RNA Alexa Fluor™ 488 HCS Assay - FAQs

查看更多产品信息 Click-iT™ RNA Alexa Fluor™ 488 HCS Assay - FAQs (C10327)

2 个常见问题解答

Can the components of the Click-iT Plus EdU Alexa Fluor Imaging Kits be used for labeling samples for flow cytometry?

Other than the EdU (Component A), DMSO (Component C), and Click-iT EdU buffer additive (Component F or G), all other components in the respective kits are not interchangeable. The types of reagents and amounts provided per kit were optimized either for imaging or flow cytometry. Using the imaging reagents may result in an excessive level of signal for flow cytometry detection and using the flow cytometry reagents may result in sub-optimal signal for imaging.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can any type of cell incorporate EU?

No. The cell must possess a pyrimidine salvage pathway; without this pathway, EU does not become phosphorylated to allow incorporation during RNA synthesis.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.