Cell type specific applicability of 5-ethynyl-2'-deoxyuridine (EdU) for dynamic proliferation assessment in flow cytometry.
AuthorsDiermeier-Daucher S, Clarke ST, Hill D, Vollmann-Zwerenz A, Bradford JA, Brockhoff G,
JournalCytometry A
PubMed ID19235202
'Using the nucleoside analogue EdU (5-ethynyl-2''-deoxyuridine) for thymidine substitution instead of BrdU (5-bromo-2''-deoxyuridine) in cell proliferation assays has recently been proposed. However, the effect of EdU on cell viability, DNA synthesis, and cell cycle progression and consequently its usability for dynamic cell proliferation analysis in vitro has not been explored. ... More
Imaging and analysis of 3D tumor spheroids enriched for a cancer stem cell phenotype.
AuthorsRobertson FM, Ogasawara MA, Ye Z, Chu K, Pickei R, Debeb BG, Woodward WA, Hittelman WN, Cristofanilli M, Barsky SH,
JournalJ Biomol Screen
PubMed ID20639504
'Tumors that display a highly metastatic phenotype contain subpopulations of cells that display characteristics similar to embryonic stem cells. These cells exhibit the ability to undergo self-renewal; slowly replicate to retain a nucleoside analog label, leading to their definition as ' ... More
Genetic mosaic analysis reveals a major role for frizzled 4 and frizzled 8 in controlling ureteric growth in the developing kidney.
AuthorsYe X, Wang Y, Rattner A, Nathans J,
JournalDevelopment
PubMed ID21343368
'The developing mammalian kidney is an attractive system in which to study the control of organ growth. Targeted mutations in the Wnt receptors frizzled (Fz) 4 and Fz8 lead to reduced ureteric bud growth and a reduction in kidney size, a phenotype previously reported for loss of Wnt11. In cell ... More
Epithelial cell-intrinsic Notch signaling plays an essential role in the maintenance of gut immune homeostasis.
AuthorsObata Y, Takahashi D, Ebisawa M, Kakiguchi K, Yonemura S, Jinnohara T, Kanaya T, Fujimura Y, Ohmae M, Hase K, Ohno H,
JournalJ Immunol
PubMed ID22279105
'Intestinal epithelial cells (IECs) have important functions as the first line of defense against diverse microorganisms on the luminal surface. Impaired integrity of IEC has been implicated in increasing the risk for inflammatory disorders in the gut. Notch signaling plays a critical role in the maintenance of epithelial integrity by ... More
Cell cycle synchronization of Escherichia coli using the stringent response, with fluorescence labeling assays for DNA content and replication.
AuthorsFerullo DJ, Cooper DL, Moore HR, Lovett ST,
JournalMethods
PubMed ID19245839
'We describe a method for synchronization of the cell cycle in the bacterium Escherichia coli. Treatment of asynchronous cultures with the amino acid analog, dl-serine hydroxamate, induces the stringent response, with concomitant arrest of DNA replication at initiation. Following release of the stringent response, cells initiate DNA replication in synchrony, ... More
miR-200c regulates FGFR-dependent epithelial proliferation via Vldlr during submandibular gland branching morphogenesis.
'The regulation of epithelial proliferation during organ morphogenesis is crucial for normal development, as dysregulation is associated with tumor formation. Non-coding microRNAs (miRNAs), such as miR-200c, are post-transcriptional regulators of genes involved in cancer. However, the role of miR-200c during normal development is unknown. We screened miRNAs expressed in the ... More
EdU, a new thymidine analogue for labelling proliferating cells in the nervous system.
AuthorsChehrehasa F, Meedeniya AC, Dwyer P, Abrahamsen G, Mackay-Sim A,
JournalJ Neurosci Methods
PubMed ID18996411
Labelling and identifying proliferating cells is central to understanding neurogenesis and neural lineages in vivo and in vitro. We present here a novel thymidine analogue, ethynyl deoxyuridine (EdU) for labelling dividing cells, detected with a fluorescent azide which forms a covalent bond via the ... More
AuthorsCronin SJ, Nehme NT, Limmer S, Liegeois S, Pospisilik JA, Schramek D, Leibbrandt A, Simoes Rde M, Gruber S, Puc U, Ebersberger I, Zoranovic T, Neely GG, von Haeseler A, Ferrandon D, Penninger JM,
JournalScience
PubMed ID19520911
Innate immunity represents the first line of defense in animals. We report a genome-wide in vivo Drosophila RNA interference screen to uncover genes involved in susceptibility or resistance to intestinal infection with the bacterium Serratia marcescens. We first employed whole-organism gene suppression, followed by tissue-specific silencing in gut epithelium or ... More
A chemical method for fast and sensitive detection of DNA synthesis in vivo.
AuthorsSalic A, Mitchison TJ,
JournalProc Natl Acad Sci U S A
PubMed ID18272492
We have developed a method to detect DNA synthesis in proliferating cells, based on the incorporation of 5-ethynyl-2'-deoxyuridine (EdU) and its subsequent detection by a fluorescent azide through a Cu(I)-catalyzed [3 + 2] cycloaddition reaction ("click" chemistry). Detection of the EdU label is highly sensitive and can be accomplished in ... More
Identification of a Role for the trans-Golgi Network in Human Papillomavirus 16 Pseudovirus Infection.
Human papillomavirus 16 (HPV16) enters its host cells by a process that most closely resembles macropinocytosis. Uncoating occurs during passage through the endosomal compartment, and the low pH encountered in this environment is essential for infection. Furin cleavage of the minor capsid protein, L2, and cyclophilin B-mediated separation of L2 ... More
A cross-platform analysis of 14,177 expression quantitative trait loci derived from lymphoblastoid cell lines.
Gene expression levels can be an important link DNA between variation and phenotypic manifestations. Our previous map of global gene expression, based on ~400K single nucleotide polymorphisms (SNPs) and 50K transcripts in 400 sib pairs from the MRCA family panel, has been widely used to interpret the results of genome-wide ... More
Disrupting galectin-1 interactions with N-glycans suppresses hypoxia-driven angiogenesis and tumorigenesis in Kaposi's sarcoma.
AuthorsCroci DO, Salatino M, Rubinstein N, Cerliani JP, Cavallin LE, Leung HJ, Ouyang J, Ilarregui JM, Toscano MA, Domaica CI, Croci MC, Shipp MA, Mesri EA, Albini A, Rabinovich GA,
JournalJ Exp Med
PubMed ID23027923
Kaposi's sarcoma (KS), a multifocal vascular neoplasm linked to human herpesvirus-8 (HHV-8/KS-associated herpesvirus [KSHV]) infection, is the most common AIDS-associated malignancy. Clinical management of KS has proven to be challenging because of its prevalence in immunosuppressed patients and its unique vascular and inflammatory nature that is sustained by viral and ... More
The H3K4me3 histone demethylase Fbxl10 is a regulator of chemokine expression, cellular morphology, and the metabolome of fibroblasts.
AuthorsJanzer A, Stamm K, Becker A, Zimmer A, Buettner R, Kirfel J,
JournalJ Biol Chem
PubMed ID22825849
Fbxl10 (Jhdm1b/Kdm2b) is a conserved and ubiquitously expressed member of the JHDM (JmjC domain-containing histone demethylase) family. Fbxl10 was implicated in the demethylation of H3K4me3 or H3K36me2 thereby removing active chromatin marks and inhibiting gene transcription. Apart from the JmjC domain, Fbxl10 consists of a CxxC domain, a PHD domain, ... More
Glucose-mediated repression of menin promotes pancreatic ß-cell proliferation.
AuthorsZhang H, Li W, Wang Q, Wang X, Li F, Zhang C, Wu L, Long H, Liu Y, Li X, Luo M, Li G, Ning G,
JournalEndocrinology
PubMed ID22166975
Menin, encoded by the Men1 gene, is responsible for ß-cell tumor formation in patients with multiple endocrine neoplasia type 1. Recently, menin has been proven to negatively regulate ß-cell proliferation during pregnancy. However, it is unclear whether menin is involved in pancreatic ß-cell proliferation in response to other physiological replication ... More
Progesterone receptor inhibits proliferation of human breast cancer cells via induction of MAPK phosphatase 1 (MKP-1/DUSP1).
AuthorsChen CC, Hardy DB, Mendelson CR,
JournalJ Biol Chem
PubMed ID22020934
The roles of progesterone (P(4)) and of progesterone receptor (PR) in development and pathogenesis of breast cancer remain unclear. In this study, we observed that treatment of T47D breast cancer cells with progestin antagonized effects of fetal bovine serum (FBS) to stimulate cell proliferation, whereas siRNA-mediated knockdown of endogenous PR ... More
Mature mice lacking Rbl2/p130 gene have supernumerary inner ear hair cells and supporting cells.
AuthorsRocha-Sanchez SM, Scheetz LR, Contreras M, Weston MD, Korte M, McGee J, Walsh EJ,
JournalJ Neurosci
PubMed ID21677172
Adult mammalian auditory hair cells (HCs) and their associated supporting cells (SCs) do not proliferate, and HC death leads to irreversible neurosensory hearing loss and balance impairment. In nonmammalian vertebrates, loss of HCs induces mitotic proliferation of adjacent nonsensory SCs and/or direct SC transdifferentiation to generate replacement cells. This results ... More
Gli3 is required for maintenance and fate specification of cortical progenitors.
AuthorsWang H, Ge G, Uchida Y, Luu B, Ahn S,
JournalJ Neurosci
PubMed ID21525285
Gli3, one of three vertebrate Gli transcription factors in Hedgehog (Hh) pathway, is processed into a repressor form (Gli3R) in the absence of Hh signal and acts as the major negative transducer of the pathway. Although the role of Gli3 in embryonic patterning has been extensively studied, its role in ... More
Interleukin-15 regulates proliferation and self-renewal of adult neural stem cells.
AuthorsGómez-Nicola D, Valle-Argos B, Pallas-Bazarra N, Nieto-Sampedro M,
JournalMol Biol Cell
PubMed ID21508317
The impact of inflammation is crucial for the regulation of the biology of neural stem cells (NSCs). Interleukin-15 (IL-15) appears as a likely candidate for regulating neurogenesis, based on its well-known mitogenic properties. We show here that NSCs of the subventricular zone (SVZ) express IL-15, which regulates NSC proliferation, as ... More
DeltaA/DeltaD regulate multiple and temporally distinct phases of notch signaling during dopaminergic neurogenesis in zebrafish.
AuthorsMahler J, Filippi A, Driever W,
JournalJ Neurosci
PubMed ID21148001
Dopaminergic neurons develop at distinct anatomical sites to form some of the major neuromodulatory systems in the vertebrate brain. Despite their relevance in neurodegenerative diseases and the interests in reconstitutive therapies from stem cells, mechanisms of the neurogenic switch from precursor populations to dopaminergic neurons are not well understood. Here, ... More
The sub-cellular localization of Sulfolobus DNA replication.
AuthorsGristwood T, Duggin IG, Wagner M, Albers SV, Bell SD,
JournalNucleic Acids Res
PubMed ID22402489
Analyses of the DNA replication-associated proteins of hyperthermophilic archaea have yielded considerable insight into the structure and biochemical function of these evolutionarily conserved factors. However, little is known about the regulation and progression of DNA replication in the context of archaeal cells. In the current work, we describe the generation ... More
A rapid and robust assay for detection of S-phase cell cycle progression in plant cells and tissues by using ethynyl deoxyuridine.
AuthorsKotogány E, Dudits D, Horváth GV, Ayaydin F,
JournalPlant Methods
PubMed ID20181034
ABSTRACT: BACKGROUND: Progress in plant cell cycle research is highly dependent on reliable methods for detection of cells replicating DNA. Frequency of S-phase cells (cells in DNA synthesis phase) is a basic parameter in studies on the control of cell division cycle and the developmental events of plant cells. Here ... More
Dkk-1 inhibits intestinal epithelial cell migration by attenuating directional polarization of leading edge cells.
AuthorsKoch S, Capaldo CT, Samarin S, Nava P, Neumaier I, Skerra A, Sacks DB, Parkos CA, Nusrat A,
JournalMol Biol Cell
PubMed ID19776352
Wnt signaling pathways regulate proliferation, motility, and survival in a variety of human cell types. Dickkopf-1 (Dkk-1) is a secreted Wnt antagonist that has been proposed to regulate tissue homeostasis in the intestine. In this report, we show that Dkk-1 is secreted by intestinal epithelial cells after wounding and that ... More
Chick embryo proliferation studies using EdU labeling.
AuthorsWarren M, Puskarczyk K, Chapman SC,
JournalDev Dyn
PubMed ID19253396
Cell proliferation studies are an important experimental tool. The most commonly used thymidine analogues, tritiated thymidine and bromodeoxyuridine (BrdU) label cells during S-phase. Both methods have significant drawbacks: low sensitivity in the case of tritiated thymidine and a denaturation step during BrdU detection that destroys most cellular epitopes, requiring careful ... More
A rapid non-radioactive technique for measurement of repair synthesis in primary human fibroblasts by incorporation of ethynyl deoxyuridine (EdU).
AuthorsLimsirichaikul S, Niimi A, Fawcett H, Lehmann A, Yamashita S, Ogi T,
JournalNucleic Acids Res
PubMed ID19179371
Xeroderma pigmentosum (XP) is an autosomal recessive genetic disorder. Afflicted patients show extreme sun-sensitivity and skin cancer predisposition. XP is in most cases associated with deficient nucleotide excision repair (NER), which is the process responsible for removing photolesions from DNA. Measuring NER activity by nucleotide incorporation into repair patches, termed ... More
METT-10, a putative methyltransferase, inhibits germ cell proliferative fate in Caenorhabditis elegans.
AuthorsDorsett M, Westlund B, Schedl T,
JournalGenetics
PubMed ID19596901
Germ-line stem cells are unique because they either self-renew through mitosis or, at a certain frequency, switch to meiosis and produce gametes. The switch from proliferation to meiosis is tightly regulated, and aberrations in switching result in either too little or too much proliferation. To understand the genetic basis of ... More
Technical Advance: The rat aorta contains resident mononuclear phagocytes with proliferative capacity and proangiogenic properties.
AuthorsZorzi P, Aplin AC, Smith KD, Nicosia RF,
JournalJ Leukoc Biol
PubMed ID20628067
Angiogenesis in the aortic ring model is preceded by activation of the immune system and impaired by ablation of adventitial macrophages. Treatment of aortic cultures with M-CSF induced extensive periaortic outgrowth of CD45(+) CD68(+) mononuclear cells with ultrastructural features of macrophages and DCs. Periaortic lysis of collagen caused many CD45(+) ... More
SAF-A Regulates Interphase Chromosome Structure through Oligomerization with Chromatin-Associated RNAs.
Authors
JournalCell
PubMed ID28622508
Long-Term Culture Captures Injury-Repair Cycles of Colonic Stem Cells.
Authors
JournalCell
PubMed ID31708126
Impaired Epidermal to Dendritic T Cell Signaling Slows Wound Repair in Aged Skin.
Authors
JournalCell
PubMed ID27863246
Human-Specific NOTCH2NL Genes Expand Cortical Neurogenesis through Delta/Notch Regulation.
Authors
JournalCell
PubMed ID29856955
FBXO44 promotes DNA replication-coupled repetitive element silencing in cancer cells.
Authors
JournalCell
PubMed ID33357448
Excessive Cell Growth Causes Cytoplasm Dilution And Contributes to Senescence.
Authors
JournalCell
PubMed ID30739799
DNA Damage Signaling Instructs Polyploid Macrophage Fate in Granulomas.
Authors
JournalCell
PubMed ID28084216
Regulation of Cell Cycle to Stimulate Adult Cardiomyocyte Proliferation and Cardiac Regeneration.
Authors
JournalCell
PubMed ID29502971
Genetic and Mechanical Regulation of Intestinal Smooth Muscle Development.
Authors
JournalCell
PubMed ID31539501
A Unique Collateral Artery Development Program Promotes Neonatal Heart Regeneration.