'The stem cells (SCs) at the bottom of intestinal crypts tightly contact niche-supporting cells and fuel the extraordinary tissue renewal of intestinal epithelia. Their fate is regulated stochastically by populational asymmetry, yet whether asymmetrical fate as a mode of SC division is relevant and whether the SC niche contains committed ... More
APC/C-CCS52A complexes control meristem maintenance in the Arabidopsis root.
AuthorsVanstraelen M, Baloban M, Da Ines O, Cultrone A, Lammens T, Boudolf V, Brown SC, De Veylder L, Mergaert P, Kondorosi E,
JournalProc Natl Acad Sci U S A
PubMed ID19553203
'Plant organs originate from meristems where stem cells are maintained to produce continuously daughter cells that are the source of different cell types. The cell cycle switch gene CCS52A, a substrate specific activator of the anaphase promoting complex/cyclosome (APC/C), controls the mitotic arrest and the transition of mitotic cycles to ... More
Dynamic temporal requirement of Wnt1 in midbrain dopamine neuron development.
AuthorsYang J, Brown A, Ellisor D, Paul E, Hagan N, Zervas M,
JournalDevelopment
PubMed ID23444360
'Wnt1-expressing progenitors generate midbrain dopamine (MbDA) and cerebellum (Cb) neurons in distinct temporal windows and from spatially discrete progenitor domains. It has been shown that Wnt1 and Lmx1a participate in a cross-regulatory loop that is utilized during MbDA neuron development. However, Wnt1 expression dynamically changes over time and precedes that ... More
The human GINS complex associates with Cdc45 and MCM and is essential for DNA replication.
AuthorsAparicio T, Guillou E, Coloma J, Montoya G, Méndez J,
JournalNucleic Acids Res
PubMed ID19223333
'The GINS complex, originally discovered in Saccharomyces cerevisiae and Xenopus laevis, binds to DNA replication origins shortly before the onset of S phase and travels with the replication forks after initiation. In this study we present a detailed characterization of the human GINS (hGINS) homolog. Using new antibodies that allow ... More
Paper-supported 3D cell culture for tissue-based bioassays.
AuthorsDerda R, Laromaine A, Mammoto A, Tang SK, Mammoto T, Ingber DE, Whitesides GM,
JournalProc Natl Acad Sci U S A
PubMed ID19846768
Fundamental investigations of human biology, and the development of therapeutics, commonly rely on 2D cell-culture systems that do not accurately recapitulate the structure, function, or physiology of living tissues. Systems for 3D cultures exist but do not replicate the spatial distributions of oxygen, metabolites, and signaling molecules found in tissues. ... More
Visualization of cell cycle in mouse embryos with Fucci2 reporter directed by Rosa26 promoter.
AuthorsAbe T, Sakaue-Sawano A, Kiyonari H, Shioi G, Inoue K, Horiuchi T, Nakao K, Miyawaki A, Aizawa S, Fujimori T,
JournalDevelopment
PubMed ID23175634
Fucci technology makes possible the distinction between live cells in the G(1) and S/G(2)/M phases by dual-color imaging. This technology relies upon ubiquitylation-mediated proteolysis, and transgenic mice expressing Fucci provide a powerful model system with which to study the coordination of the cell cycle and development. The mice were initially ... More
Retinal horizontal cells lacking Rb1 sustain persistent DNA damage and survive as polyploid giant cells.
AuthorsDonovan SL, Corbo JC,
JournalMol Biol Cell
PubMed ID23015754
The retinoblastoma tumor susceptibility gene, Rb1, is a key regulator of the cell cycle, and mutations in this gene have been found in many human cancers. Prior studies showed that retina-specific knockout of Rb1 in the mouse results in the formation of abnormally large horizontal cells, but the development, fate, ... More
Dendritic cell-nerve clusters are sites of T cell proliferation in allergic airway inflammation.
AuthorsVeres TZ, Shevchenko M, Krasteva G, Spies E, Prenzler F, Rochlitzer S, Tschernig T, Krug N, Kummer W, Braun A,
JournalAm J Pathol
PubMed ID19179611
Interactions between T cells and dendritic cells in the airway mucosa precede secondary immune responses to inhaled antigen. The purpose of this study was to identify the anatomical locations where dendritic cell-T cell interactions occur, resulting in T cells activation by dendritic cells. In a mouse model of allergic airway ... More
Distinct populations of quiescent and proliferative pancreatic beta-cells identified by HOTcre mediated labeling.
AuthorsHesselson D, Anderson RM, Beinat M, Stainier DY,
JournalProc Natl Acad Sci U S A
PubMed ID19706417
Pancreatic beta-cells are critical regulators of glucose homeostasis, and they vary dramatically in their glucose stimulated metabolic response and levels of insulin secretion. It is unclear whether these parameters are influenced by the developmental origin of individual beta-cells. Using HOTcre, a Cre-based genetic switch that uses heat-induction to precisely control ... More
Large T antigen promotes JC virus replication in G2-arrested cells by inducing ATM- and ATR-mediated G2 checkpoint signaling.
AuthorsOrba Y, Suzuki T, Makino Y, Kubota K, Tanaka S, Kimura T, Sawa H,
JournalJ Biol Chem
PubMed ID19903823
Large T antigen (TAg) of the human polyomavirus JC virus (JCV) possesses DNA binding and helicase activities, which, together with various cellular proteins, are required for replication of the viral genome. We now show that JCV-infected cells expressing TAg accumulate in the G(2) phase of the cell cycle as a ... More
Whole-body integration of gene expression and single-cell morphology.
Authors
JournalCell
PubMed ID34380046
Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease.
Authors
JournalCell
PubMed ID30270042
Rare Pulmonary Neuroendocrine Cells Are Stem Cells Regulated by Rb, p53, and Notch.
Authors
JournalCell
PubMed ID31585080
FXR Regulates Intestinal Cancer Stem Cell Proliferation.
Authors
JournalCell
PubMed ID30794774
Dual ifgMosaic: A Versatile Method for Multispectral and Combinatorial Mosaic Gene-Function Analysis.
Authors
JournalCell
PubMed ID28802047
A Sacrifice-for-Survival Mechanism Protects Root Stem Cell Niche from Chilling Stress.
Authors
JournalCell
PubMed ID28648662
A Patient-Derived Glioblastoma Organoid Model and Biobank Recapitulates Inter- and Intra-tumoral Heterogeneity.
Authors
JournalCell
PubMed ID31883794
Regulation of Cell Cycle to Stimulate Adult Cardiomyocyte Proliferation and Cardiac Regeneration.
Authors
JournalCell
PubMed ID29502971
A Common Embryonic Origin of Stem Cells Drives Developmental and Adult Neurogenesis.
Authors
JournalCell
PubMed ID30929900
Omega-3 Fatty Acids Activate Ciliary FFAR4 to Control Adipogenesis.