Click-iT™ 蛋白富集试剂盒，用于点击化学法捕获叠氮化物修饰的蛋白
Click-iT™ 蛋白富集试剂盒，用于点击化学法捕获叠氮化物修饰的蛋白
Invitrogen™

Click-iT™ 蛋白富集试剂盒，用于点击化学法捕获叠氮化物修饰的蛋白

Click-iT™ 蛋白质富集试剂盒用于在炔烃琼脂糖树脂上基于 click 化学反应实现叠氮化物修饰蛋白质的高效捕获。优于基于生物素或凝集素的富集方法。是蛋白质组学、生物标记物发现、翻译后修饰 (PTM) 分析等应用的理想选择了解更多信息
Have Questions?
货号数量
C104161 个试剂盒
货号 C10416
价格（CNY)
8,999.00
Each
添加至购物车
数量:
1 个试剂盒
价格（CNY)
8,999.00
Each
添加至购物车
Click-iT™ 蛋白质富集试剂盒用于在炔烃琼脂糖树脂上基于 click 化学反应实现叠氮化物修饰蛋白质的高效捕获。优于基于生物素或凝集素的富集方法。是蛋白质组学、生物标记物发现、翻译后修饰 (PTM) 分析等应用的理想选择。可以通过代谢补料、酶促添加或化学修饰进行叠氮化物修饰。利用提供的炔烃琼脂糖树脂，从复杂的蛋白质提取物中富集通过 Click 化学反应形成的叠氮化物修饰蛋白质或其翻译后修饰形式。在通过铜催化的 click 化学反应锚定至树脂后，进行充分洗涤得到高度富集的新生蛋白质分子，然后通过质谱法进一步表征。与现有的生物素方法相比，炔烃琼脂糖树脂的性能提升了 >8 倍，生物素的信噪比 = 3，而 Click-iT 树脂的信噪比 = 25。

完善的上游 MS 样本制备技术，可用于观察整体蛋白表达的变化和生物标记物分析。

这种新型 Click-iT™ 富集树脂具有许多独特优势，包括：
  • 对多个亚类的翻译后修饰 (PTM) 蛋白质和新合成蛋白质进行整体差异表达谱分析
  • 通过消除非特异性结合和提高选择性来增加信噪比，从而改善低丰度蛋白质的检测
  • 加速 PTM 位点鉴定，实现功能研究的更快启动
  • 与广泛使用的 MS 技术完全兼容，包括 iTRAC 和 ICAT。

细胞生物学与蛋白质化学无缝集成。

经济实惠、简单易用、无需特殊设备。

如需了解您的细胞中有什么新成分，请使用 Click-iT™

Click-iT™：一种反应、无限可能。
仅供科研使用。不可用于诊断程序。
规格
产品规格离心柱
产品线Click-iT™
产品类型蛋白富集试剂盒
数量1 个试剂盒
运输条件室温
Unit SizeEach
内容与储存
存储在 2°C 到 8°C。建议干燥储存。避免长时间暴露于光下，可在光下做短时间操作。

常见问题解答 (FAQ)

What is the expected peptide yield for samples prepared with the Click-iT Protein Enrichment Kit, for click chemistry capture of azide-modified proteins (Cat. No. C10416)?

The yield of purified peptides from samples prepared with the Click-iT Protein Enrichment Kit, for click chemistry capture of azide-modified proteins (Cat. No. C10416), strongly depends on the sample type, the incorporation efficiency of the azide analog used, and the amount of lysate added to the resin. In the examples presented in the posters linked below, we were able to recover approximately 25-50 µg of purified peptides from about 0.5 to 8 mg of cell lysate, using approximately 100 µL of resin.

Click chemistry enrichment of cell surface glycoproteins

A universal click chemistry-based enrichment column for the identification of multiple subclasses of PTM-modified proteins and newly synthesized proteins

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How can I confirm the protein is enriched in samples prepared with the Click-iT Protein Enrichment Kit, for click chemistry capture of azide-modified proteins (Cat. No. C10416)?

To confirm the protein is enriched in samples prepared with the Click-iT Protein Enrichment Kit, for click chemistry capture of azide-modified proteins (Cat. No. C10416) we recommend saving the flow through from the column along with a small aliquot of the initial cell lysate and running these on a gel together. This will show the depletion of the azide-labeled proteins from the lysate. Please refer to the posters below that show examples of how we have analyzed peptide recovery (or depletion of the lysate):

Click chemistry enrichment of cell surface glycoproteins

A universal click chemistry-based enrichment column for the identification of multiple subclasses of PTM-modified proteins and newly synthesized proteins

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.