The airway epithelium nucleotide-binding domain and leucine-rich repeat protein 3 inflammasome is activated by urban particulate matter.
AuthorsHirota JA, Hirota SA, Warner SM, Stefanowicz D, Shaheen F, Beck PL, Macdonald JA, Hackett TL, Sin DD, Van Eeden S, Knight DA,
JournalJ Allergy Clin Immunol
PubMed ID22227418
The airway epithelium is the first line of defense against inhaled insults and therefore must be capable of coordinating appropriate inflammatory and immune responses. We sought to test the hypothesis that the nucleotide-binding domain and leucine-rich repeat protein 3 (NLRP3) inflammasome, an intracellular danger-sensing complex, plays a critical role in ... More
Combination Small Molecule MEK and PI3K Inhibition Enhances Uveal Melanoma Cell Death in a Mutant GNAQ- and GNA11-Dependent Manner.
AuthorsKhalili JS, Yu X, Wang J, Hayes BC, Davies MA, Lizee G, Esmaeli B, Woodman SE,
JournalClin Cancer Res
PubMed ID22733540
Activating Q209L/P mutations in GNAQ or GNA11 (GNAQ/11) are present in approximately 80% of uveal melanomas. Mutant GNAQ/11 are not currently therapeutically targetable. Inhibiting key down-stream effectors of GNAQ/11 represents a rational therapeutic approach for uveal melanomas that harbor these mutations. The mitogen-activated protein/extracellular signal-regulated kinase/mitogen-activated protein kinase (MEK/MAPK) and ... More
Mutant BRAF induces DNA strand breaks, activates DNA damage response pathway, and up-regulates glucose transporter-1 in nontransformed epithelial cells.
AuthorsSheu JJ, Guan B, Tsai FJ, Hsiao EY, Chen CM, Seruca R, Wang TL, Shih IeM,
JournalAm J Pathol
PubMed ID22227015
'Although the oncogenic functions of activating BRAF mutations have been clearly demonstrated in human cancer, their roles in nontransformed epithelial cells remain largely unclear. Investigating the cellular response to the expression of mutant BRAF in nontransformed epithelial cells is fundamental to the understanding of the roles of BRAF in cancer ... More
Mechanisms of programmed cell death signaling in hair cells and support cells post-electrode insertion trauma.
AuthorsEshraghi AA, Lang DM, Roell J, Van De Water TR, Garnham C, Rodrigues H, Guardiola M, Gupta C, Mittal J,
Journal
PubMed ID25761716
'Programmed cell death (PCD) initially starts in the support cells (SCs) after electrode insertion trauma (EIT), followed by PCD in hair cells (HCs). Activation of caspase-3 was observed only in SCs. Protecting both SCs and HCs with selective otoprotective drugs at an early stage post implantation may help to preserve ... More
OPA1 Mutation and Late-Onset Cardiomyopathy: Mitochondrial Dysfunction and mtDNA Instability.
AuthorsChen L, Liu T, Tran A, Lu X, Tomilov AA, Davies V, Cortopassi G, Chiamvimonvat N, Bers DM, Votruba M, Knowlton AA,
JournalJ Am Heart Assoc
PubMed ID23316298
'Mitochondrial fusion protein mutations are a cause of inherited neuropathies such as Charcot-Marie-Tooth disease and dominant optic atrophy. Previously we reported that the fusion protein optic atrophy 1 (OPA1) is decreased in heart failure. We investigated cardiac function, mitochondrial function, and mtDNA stability in a mouse model of the disease ... More
Angiotensin-(1-7) administration reduces oxidative stress in diabetic bone marrow.
AuthorsMordwinkin NM, Meeks CJ, Jadhav SS, Espinoza T, Roda N, diZerega GS, Louie SG, Rodgers KE,
JournalEndocrinology
PubMed ID22434085
'Diabetics have an increased risk of developing cardiovascular disease, in part due to oxidative stress, resulting in endothelial nitric oxide synthase (eNOS) dysfunction. Studies have demonstrated that angiotensin-(1-7) [Ang-(1-7)] can activate eNOS activity. Because the bone marrow is a primary source of a number of progenitors important in physiological homeostasis ... More
Ultrasound stimulation restores impaired neovascularization-related capacities of human circulating angiogenic cells.
AuthorsToyama Y, Sasaki KI, Tachibana K, Ueno T, Kajimoto H, Yokoyama S, Ohtsuka M, Koiwaya H, Nakayoshi T, Mitsutake Y, Chibana H, Itaya N, Imaizumi T,
JournalCardiovasc Res
PubMed ID22641844
'AIMS: Unsatisfactory effects of therapeutic angiogenesis in critical limb ischaemia may be ascribed to use of circulating angiogenic cells (CACs) derived from atherosclerotic patients with impaired neovascularization-related capacities. We tested whether ultrasound cell stimulation can restore the impaired capacities. METHODS AND RESULTS: During culture of human peripheral blood-derived mononuclear cells ... More
Macrophage autophagy plays a protective role in advanced atherosclerosis.
AuthorsLiao X, Sluimer JC, Wang Y, Subramanian M, Brown K, Pattison JS, Robbins J, Martinez J, Tabas I,
JournalCell Metab
PubMed ID22445600
'In advanced atherosclerosis, macrophage apoptosis coupled with defective phagocytic clearance of the apoptotic cells (efferocytosis) promotes plaque necrosis, which precipitates acute atherothrombotic cardiovascular events. Oxidative and endoplasmic reticulum (ER) stress in macrophages are important causes of advanced lesional macrophage apoptosis. We now show that proapoptotic oxidative/ER stress inducers trigger another ... More
'J. Neurochem. (2012) 122, 221-229. ABSTRACT: Spreading depression (SD), the likely cause of migraine aura and perhaps migraine, is triggered by widespread and unfettered neuronal hyperexcitability. Migraine and the initiating hyperexcitability of seizure, which involve oxidative stress (OS), are likely interrelated. Environmental enrichment (EE) decreases seizure and can reduce migraine. ... More
Adenosine triphosphate regulates NADPH oxidase activity leading to hydrogen peroxide production and COX-2/PGE2 expression in A549 cells.
AuthorsLin CC, Lee IT, Wu WL, Lin WN, Yang CM,
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID22773695
'Adenosine triphosphate regulates NADPH oxidase activity leading to hydrogen peroxide production and COX-2/PGE(2) expression in A549 cells. Am J Physiol Lung Cell Mol Physiol ___:L___-L___, 2012. Non-small cell lung carcinoma (NSCLC) accounts for most of all lung cancers, which is the leading cause of mortality in human being. High level ... More
Evidence of the in vitro genotoxicity of methyl-pyrazole pesticides in human cells.
AuthorsGraillot V, Tomasetig F, Cravedi JP, Audebert M,
JournalMutat Res
PubMed ID22743356
'Consumers are exposed daily to several pesticide residues in food, which can be of potential concern for human health. Based on a previous study dealing with exposure of the French population to pesticide residues via the food, we selected 14 pesticides frequently found in foodstuffs, on the basis of their ... More
Inhibitor of apoptosis proteins limit RIP3 kinase-dependent interleukin-1 activation.
AuthorsVince JE, Wong WW, Gentle I, Lawlor KE, Allam R, O'Reilly L, Mason K, Gross O, Ma S, Guarda G, Anderton H, Castillo R, Häcker G, Silke J, Tschopp J,
JournalImmunity
PubMed ID22365665
Interleukin-1ß (IL-1ß) is a potent inflammatory cytokine that is usually cleaved and activated by inflammasome-associated caspase-1. To determine whether IL-1ß activation is regulated by inhibitor of apoptosis (IAP) proteins, we treated macrophages with an IAP-antagonist ... More
Ex vivo imaging of excised tissue using vital dyes and confocal microscopy.
AuthorsJohnson S, Rabinovitch P,
JournalCurr Protoc Cytom
PubMed ID22752953
Vital dyes routinely used for staining cultured cells can also be used to stain and image live tissue slices ex vivo. Staining tissue with vital dyes allows researchers to collect structural and functional data simultaneously and can be used for qualitative or quantitative fluorescent image collection. The protocols presented here ... More
NAD+ and SIRT3 control microtubule dynamics and reduce susceptibility to antimicrotubule agents.
AuthorsHarkcom WT, Ghosh AK, Sung MS, Matov A, Brown KD, Giannakakou P, Jaffrey SR,
Journal
PubMed ID24889606
Nicotinamide adenine dinucleotide (NAD(+)) is an endogenous enzyme cofactor and cosubstrate that has effects on diverse cellular and physiologic processes, including reactive oxygen species generation, mitochondrial function, apoptosis, and axonal degeneration. A major goal is to identify the NAD(+)-regulated cellular pathways that may mediate these effects. Here we show that ... More
Epidermal cells help coordinate leukocyte migration during inflammation through fatty acid-fuelled matrix metalloproteinase production.
In addition to satisfying the metabolic demands of cells, mitochondrial metabolism helps regulate immune cell function. To date, such cell-intrinsic metabolic-immunologic cross-talk has only been described operating in cells of the immune system. Here we show that epidermal cells utilize fatty acid ß-oxidation to fuel their contribution to the immune ... More
Silencing of enzymes involved in ceramide biosynthesis causes distinct global alterations of lipid homeostasis and gene expression.
AuthorsRuangsiriluk W, Grosskurth SE, Ziemek D, Kuhn M, des Etages SG, Francone OL,
JournalJ Lipid Res
PubMed ID22628619
Dysregulation of ceramide synthesis has been associated with metabolic disorders such as atherosclerosis and diabetes. We examined the changes in lipid homeostasis and gene expression in Huh7 hepatocytes when the synthesis of ceramide is perturbed by knocking down serine pal mitoyltransferase subunits 1, 2, and 3 (SPTLC123) or dihydroceramide desaturase ... More
CTGF drives autophagy, glycolysis and senescence in cancer-associated fibroblasts via HIF1 activation, metabolically promoting tumor growth.
Previous studies have demonstrated that loss of caveolin-1 (Cav-1) in stromal cells drives the activation of the TGF-ß signaling, with increased transcription of TGF-ß target genes, such as connective tissue growth factor (CTGF). In addition, loss of stromal Cav-1 results in the metabolic reprogramming of cancer-associated fibroblasts, with the induction ... More
Deregulated MYC expression induces dependence upon AMPK-related kinase 5.
Deregulated expression of the MYC oncoprotein contributes to the genesis of many human tumours, yet strategies to exploit this for a rational tumour therapy are scarce. MYC promotes cell growth and proliferation, and alters cellular metabolism to enhance the provision of precursors for phospholipids and cellular macromolecules. Here we show ... More
Mild Hyperthermia Promotes and Accelerates Development and Maturation of Erythroid Cells.
AuthorsTounkara FK, Dumont N, Fournier S, Boyer L, Nadeau P, Pineault N,
JournalStem Cells Dev
PubMed ID22564002
Hyperthermia treatment has at times been associated with increased platelet levels in humans. The heat shock protein HSP70, which can be induced by hyperthermia in megakaryocytes and erythrocytes, was recently shown to protect GATA-1 from degradation and to be required for erythroid differentiation. Based on these findings, we hypothesize that ... More
Bmi1 confers resistance to oxidative stress on hematopoietic stem cells.
AuthorsNakamura S, Oshima M, Yuan J, Saraya A, Miyagi S, Konuma T, Yamazaki S, Osawa M, Nakauchi H, Koseki H, Iwama A,
JournalPLoS One
PubMed ID22606246
The polycomb-group (PcG) proteins function as general regulators of stem cells. We previously reported that retrovirus-mediated overexpression of Bmi1, a gene encoding a core component of polycomb repressive complex (PRC) 1, maintained self-renewing hematopoietic stem cells (HSCs) during long-term culture. However, the effects of overexpression of Bmi1 on HSCs in ... More
Two-photon fluorescence microscopy imaging of cellular oxidative stress using profluorescent nitroxides.
AuthorsAhn HY, Fairfull-Smith KE, Morrow BJ, Lussini V, Kim B, Bondar MV, Bottle SE, Belfield KD,
JournalJ Am Chem Soc
PubMed ID22380794
A range of varying chromophore nitroxide free radicals and their nonradical methoxyamine analogues were synthesized and their linear photophysical properties examined. The presence of the proximate free radical masks the chromophore's usual fluorescence emission, and these species are described as profluorescent. Two nitroxides incorporating anthracene and fluorescein chromophores (compounds 7 ... More
Intercellular transfer of apoptotic signals via electrofusion.
AuthorsPark JS, Lee W, McCulloch CA,
JournalExp Cell Res
PubMed ID22426198
We determined whether cells that are induced to undergo anoikis by matrix detachment can initiate apoptosis in healthy cells following electroporation-induced fusion. Separate populations of MDCK cells undergoing anoikis and stained with FITC-annexin or viable MDCK cells that were labeled with spectrally discrete fluorescent beads were electroporated. Cells were analyzed ... More
Tumor suppressive microRNA-1 mediated novel apoptosis pathways through direct inhibition of splicing factor serine/arginine-rich 9 (SRSF9/SRp30c) in bladder cancer.
AuthorsYoshino H, Enokida H, Chiyomaru T, Tatarano S, Hidaka H, Yamasaki T, Gotannda T, Tachiwada T, Nohata N, Yamane T, Seki N, Nakagawa M,
JournalBiochem Biophys Res Commun
PubMed ID22178073
We have previously found that restoration of tumor suppressive microRNA-1 (miR-1), induced cell apoptosis in bladder cancer (BC) cell lines. However, the apoptosis mechanism induced by miR-1 was not fully elucidated. Alternative splicing of mRNA precursors provides cancer cells with opportunities to translate many oncogenic protein variants, which promote cell ... More
Transglutaminase 2-mediated activation of ß-catenin signaling has a critical role in warfarin-induced vascular calcification.
AuthorsBeazley KE, Deasey S, Lima F, Nurminskaya MV,
JournalArterioscler Thromb Vasc Biol
PubMed ID22034513
Accumulating experimental evidence implicates ß-catenin signaling and enzyme transglutaminase 2 (TG2) in the progression of vascular calcification, and our previous studies have shown that TG2 can activate ß-catenin signaling in vascular smooth muscle cells (VSMCs). Here we investigated the role of the TG2/ß-catenin signaling axis in vascular calcification induced by ... More
Increased radiosensitivity in cells of two human cell lines treated with bystander medium from irradiated repair-deficient cells.
AuthorsMothersill C, Seymour RJ, Seymour CB,
JournalRadiat Res
PubMed ID16392959
Radiation-induced bystander factors have been shown to be more toxic if they are from medium harvested from irradiated repair-deficient cells. The aim of this study was to test the hypothesis that the radiosensitivity of repair-proficient cells can be increased by exposing them to medium-borne factors harvested from sensitive cells and ... More
Development of poly(ethylene glycol) hydrogels for salivary gland tissue engineering applications.
Over 40,000 patients are diagnosed with head and neck cancers annually in the US with the vast majority receiving radiation therapy. Salivary glands are irreparably damaged by radiation therapy resulting in xerostomia, which severely affects patient quality of life. Cell-based therapies have shown some promise in mouse models of radiation-induced ... More
Oxidative stress triggers cytokinesis failure in hepatocytes upon isolation.
AuthorsTormos AM, Taléns-Visconti R, Bonora-Centelles A, Pérez S, Sastre J,
Journal
PubMed ID25744598
Primary hepatocytes are highly differentiated cells and proliferatively quiescent. However, the stress produced during liver digestion seems to activate cell cycle entry by proliferative/dedifferentiation programs that still remain unclear. The aim of this work was to assess whether the oxidative stress associated with hepatocyte isolation affects cell cycle and particularly ... More
DNA Damage: A Sensible Mediator of the Differentiation Decision in Hematopoietic Stem Cells and in Leukemia.
AuthorsWeiss CN, Ito K,
Journal
PubMed ID25789504
In the adult, the source of functionally diverse, mature blood cells are hematopoietic stem cells, a rare population of quiescent cells that reside in the bone marrow niche. Like stem cells in other tissues, hematopoietic stem cells are defined by their ability to self-renew, in order to maintain the stem ... More
Identification of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase as a novel autophagy regulator by high content shRNA screening.
AuthorsStrohecker AM, Joshi S, Possemato R, Abraham RT, Sabatini DM, White E,
Journal
PubMed ID25772235
Deregulation of autophagy has been linked to multiple degenerative diseases and cancer, thus the identification of novel autophagy regulators for potential therapeutic intervention is important. To meet this need, we developed a high content image-based short hairpin RNA screen monitoring levels of the autophagy substrate p62/SQSTM1. We identified 186 genes ... More
Effect of uraemia on endothelial cell damage is mediated by the integrin linked kinase pathway.
AuthorsGarcía-Jérez A, Luengo A, Carracedo J, Ramírez-Chamond R, Rodriguez-Puyol D, Rodriguez-Puyol M, Calleros L,
Journal
PubMed ID25398526
Patients with chronic kidney disease have a higher risk of developing cardiovascular diseases than the general population. Their vascular endothelium is dysfunctional, among other things, because it is permanently exposed to uraemic toxins, several of which have poor clearance by conventional dialysis. Recent studies have demonstrated the important role of ... More
Pathogenic bacterial species associated with endodontic infection evade innate immune control by disabling neutrophils.
AuthorsMatsui A, Jin JO, Johnston CD, Yamazaki H, Houri-Haddad Y, Rittling SR,
Journal
PubMed ID25024367
Endodontic infections, in which oral bacteria access the tooth pulp chamber, are common and do not resolve once established. To investigate the effects of these infections on the innate immune response, we established a mouse subcutaneous chamber model, where a mixture of four oral pathogens commonly associated with these infections ... More
Skeletal muscle stem cells adopt a dormant cell state post mortem and retain regenerative capacity.
AuthorsLatil M, Rocheteau P, Châtre L, Sanulli S, Mémet S, Ricchetti M, Tajbakhsh S, Chrétien F,
JournalNat Commun
PubMed ID22692546
The accessibility to stem cells from healthy or diseased individuals, and the maintenance of their potency are challenging issues for stem cell biology. Here we report the isolation of viable and functional skeletal myogenic cells from humans up to 17 days, and mice up to 14 days post mortem, much ... More
Decrease of miR-146b-5p in monocytes during obesity is associated with loss of the anti-inflammatory but not insulin signaling action of adiponectin.
AuthorsHulsmans M, Van Dooren E, Mathieu C, Holvoet P,
JournalPLoS One
PubMed ID22393448
Low adiponectin, a well-recognized antidiabetic adipokine, has been associated with obesity-related inflammation, oxidative stress and insulin resistance. Globular adiponectin is an important regulator of the interleukin-1 receptor-associated kinase (IRAK)/NF?B pathway in monocytes of obese subjects. It protects against inflammation and oxidative stress by inducing IRAK3. microRNA (miR)-146b-5p inhibits NF?B-mediated inflammation ... More
Increased Vascular Permeability in the Bone Marrow Microenvironment Contributes to Disease Progression and Drug Response in Acute Myeloid Leukemia.
Authors
JournalCancer Cell
PubMed ID28870739
Synthesis of TPEN variants to improve cancer cells selective killing capacity.
AuthorsSchaefer-Ramadan S, Barlog M, Roach J, Al-Hashimi M, Bazzi HS, Machaca K
JournalBioorg Chem
PubMed ID30913468
'TPEN is an amino chelator of transition metals that is effective at the cellular and whole organism levels. Although TPEN of often used as a selective zinc chelators, it has affinity for copper and iron and has been shown to chelate both biologically. We have previously shown that TPEN selectively ... More
Computational flow cytometry reveals that cryopreservation induces spermptosis but subpopulations of spermatozoa may experience capacitation-like changes.
AuthorsOrtega-Ferrusola C, Anel-López L, Martín-Muñoz P, Ortíz-Rodríguez JM, Gil MC, Alvarez M, de Paz P, Ezquerra LJ, Masot AJ, Redondo E, Anel L, Peña FJ
JournalReproduction
PubMed ID27965398
'The reduced lifespan of cryopreserved spermatozoa in the mare reproductive tract has been attributed to both capacitative and apoptotic changes. However, there is a lack of studies investigating both phenomena simultaneously. In order to improve our knowledge in this particular point, we studied in raw and frozen-thawed samples apoptotic and ... More
High Fructose Drives the Serine Synthesis Pathway in Acute Myeloid Leukemic Cells.
Authors
JournalCell Metab
PubMed ID33357456
Inhibition of VDAC1 Protects Against Glutamate-Induced Oxytosis and Mitochondrial Fragmentation in Hippocampal HT22 Cells.
AuthorsNagakannan P, Islam MI, Karimi-Abdolrezaee S, Eftekharpour E
JournalCell Mol Neurobiol
PubMed ID30421242
'The involvement of glutamate in neuronal cell death in neurodegenerative diseases and neurotrauma is mediated through excitotoxicity or oxytosis. The latter process induces oxidative stress via glutamate-mediated inhibition of cysteine transporter xCT, leading to depletion of the cellular glutathione pool. Mitochondrial damage, loss of mitochondrial membrane potential (MMP), and depletion ... More
Solid Lipid Curcumin Particles Induce More DNA Fragmentation and Cell Death in Cultured Human Glioblastoma Cells than Does Natural Curcumin.
AuthorsMaiti P, Al-Gharaibeh A, Kolli N, Dunbar GL
JournalOxid Med Cell Longev
PubMed ID29359011
'Despite recent advancements in cancer therapies, glioblastoma multiforme (GBM) remains largely incurable. Curcumin (Cur), a natural polyphenol, has potent anticancer effects against several malignancies, including metastatic brain tumors. However, its limited bioavailability reduces its efficiency for treating GBM. Recently, we have shown that solid lipid Cur particles (SLCPs) have greater ... More
Inhibition of Amino Acid Metabolism Selectively Targets Human Leukemia Stem Cells.
Authors
JournalCancer Cell
PubMed ID30423294
Venetoclax with azacitidine disrupts energy metabolism and targets leukemia stem cells in patients with acute myeloid leukemia.
Authors
JournalNat Med
PubMed ID30420752
Targeting CBLB as a potential therapeutic approach for disseminated candidiasis.
Authors
JournalNat Med
PubMed ID27428899
Fibroblasts Mobilize Tumor Cell Glycogen to Promote Proliferation and Metastasis.
Authors
JournalCell Metab
PubMed ID30174305
Chemotherapy-induced transposable elements activate MDA5 to enhance haematopoietic regeneration.
Authors
JournalNat Cell Biol
PubMed ID34253898
In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8+ T cell fate decisions.
Authors
JournalCell
PubMed ID33636132
Colony stimulating factor-1 receptor is a central component of the foreign body response to biomaterial implants in rodents and non-human primates.
Authors
JournalNat Mater
PubMed ID28319612
Targeting DGAT1 Ameliorates Glioblastoma by Increasing Fat Catabolism and Oxidative Stress.
Authors
JournalCell Metab
PubMed ID32559414
Age-Related Oxidative Changes in Primary Porcine Fibroblasts Expressing Mutated Huntingtin.
AuthorsSmatlikova P, Askeland G, Vaskovicova M, Klima J, Motlik J, Eide L, Ellederová Z
JournalNeurodegener Dis
PubMed ID31167196
Huntington's disease (HD) is a devastating neurodegenerative disorder caused by CAG triplet expansions in the huntingtin gene. Oxidative stress is linked to HD pathology, although it is not clear whether this is an effect or a mediator of disease. The transgenic (TgHD) minipig expresses the N-terminal part of human-mutated huntingtin ... More
Cysteine depletion targets leukemia stem cells through inhibition of electron transport complex II.
AuthorsJones CL, Stevens BM, D'Alessandro A, Culp-Hill R, Reisz JA, Pei S, Gustafson A, Khan N, DeGregori J, Pollyea DA, Jordan CT
JournalBlood
PubMed ID31101624
We have previously demonstrated that oxidative phosphorylation is required for the survival of human leukemia stem cells (LSCs) from patients with acute myeloid leukemia (AML). More recently, we demonstrated that LSCs in patients with de novo AML rely on amino acid metabolism to drive oxidative phosphorylation. Notably, although overall levels ... More
FCPR03, a novel phosphodiesterase 4 inhibitor, alleviates cerebral ischemia/reperfusion injury through activation of the AKT/GSK3ß/ ß-catenin signaling pathway.
AuthorsXu B, Wang T, Xiao J, Dong W, Wen HZ, Wang X, Qin Y, Cai N, Zhou Z, Xu J, Wang H
JournalBiochem Pharmacol
PubMed ID30797872
Inhibition of phosphodiesterase 4 (PDE4) is a promising strategy for the treatment of ischemic stroke. However, the side effects of nausea and vomiting from the current PDE4 inhibitors have limited their clinical applications. FCPR03 is a novel PDE4 inhibitor with little emetic potential. This study aimed to investigate the effects ... More
Rosiglitazone in the thawing medium improves mitochondrial function in stallion spermatozoa through regulating Akt phosphorylation and reduction of caspase 3.
AuthorsOrtiz-Rodriguez JM, Balao da Silva C, Masot J, Redondo E, Gazquez A, Tapia JA, Gil C, Ortega-Ferrusola C, Peña FJ
JournalPLoS One
PubMed ID31276504
The population of stallion spermatozoa that survive thawing experience compromised mitochondrial functionality and accelerated senescence, among other changes. It is known that stallion spermatozoa show very active oxidative phosphorylation that may accelerate sperm senescence through increased production of reactive oxygen species. Rosiglitazone has been proven to enhance the glycolytic capability ... More
Tail loss and telomeres: consequences of large-scale tissue regeneration in a terrestrial ectotherm.
AuthorsFitzpatrick LJ, Olsson M, Parsley LM, Pauliny A, While GM, Wapstra E
JournalBiol Lett
PubMed ID31288685
Large-scale tissue regeneration has potential consequences for telomere length through increases in cell division and changes in metabolism which increase the potential for oxidative stress damage to telomeres. The effects of regeneration on telomere dynamics have been studied in fish and marine invertebrates, but the literature is scarce for terrestrial ... More