Polybrene inhibits human mesenchymal stem cell proliferation during lentiviral transduction.
AuthorsLin P, Correa D, Lin Y, Caplan AI,
JournalPLoS One
PubMed ID21887340
'Human mesenchymal stem cells (hMSCs) can be engineered to express specific genes, either for their use in cell-based therapies or to track them in vivo over long periods of time. To obtain long-term expression of these genes, a lentivirus- or retrovirus-mediated cell transduction is often used. However, given that the ... More
Identifying disruptors of male germ cell development by small molecule screening in ex vivo gonad cultures.
AuthorsWakeling SI, Miles DC, Western PS,
JournalBMC Res Notes
PubMed ID23631647
'Germ cell development involves formation of the spermatogenic or oogenic lineages from the bipotential primordial germ cells. Signaling mechanisms in the fetal testis and ovary determine whether germ cells enter the male or female developmental pathway, respectively. These signaling processes underpin an important phase of germ cell development, disruption of ... More
Constitutive phosphorylation of GATA-1 at serine²6 attenuates the colony-forming activity of erythrocyte-committed progenitors.
AuthorsLin KR, Li CL, Yen JJ, Yang-Yen HF,
Journal
PubMed ID23717580
We previously reported that IL-3 signaling induces phosphorylation of GATA-1 at the serine²6 position, which contributes to IL-3-mediated anti-apoptotic response. Here, we demonstrate that phosphorylation of GATA-1 at serine²6 is also transiently induced in cells of the erythroid lineage (primary erythroblasts and erythrocyte-committed progenitors [EPs]) by erythropoietin (EPO), the principal ... More
Aeroallergen challenge promotes dendritic cell proliferation in the airways.
AuthorsVeres TZ, Voedisch S, Spies E, Valtonen J, Prenzler F, Braun A,
JournalJ Immunol
PubMed ID23267021
Aeroallergen provocation induces the rapid accumulation of CD11c(+)MHC class II (MHC II)(+) dendritic cells (DCs) in the lungs, which is driven by an increased recruitment of blood-derived DC precursors. Recent data show, however, that well-differentiated DCs proliferate in situ in various tissues. This may also contribute to their allergen-induced expansion; ... More