Counteracting autophagy overcomes resistance to everolimus in mantle cell lymphoma.
AuthorsRosich L, Xargay-Torrent S, López-Guerra M, Campo E, Colomer D, Roué G,
JournalClin Cancer Res
PubMed ID22879389
'Mantle cell lymphoma (MCL) is an aggressive B-lymphoid neoplasm with poor response to conventional chemotherapy and short survival. The phosphatidylinositol 3-kinase/Akt/mTOR survival pathway is constitutively activated in MCL cells, thereby making the mTOR inhibition an attractive therapeutic strategy. The first clinical studies of everolimus (RAD001), an mTOR inhibitor, in relapsed ... More
The cytotoxicity of bupivacaine, ropivacaine, and mepivacaine on human chondrocytes and cartilage.
AuthorsBreu A, Rosenmeier K, Kujat R, Angele P, Zink W,
Journal
PubMed ID23749443
'Intraarticular injections of local anesthetics are frequently used as part of multimodal pain regimens. However, recent data suggest that local anesthetics affect chondrocyte viability. In this study, we assessed the chondrotoxic effects of mepivacaine, ropivacaine, and bupivacaine. We hypothesized that specific cytotoxic potencies directly correlate with analgesic potencies, and that ... More
Proteomic analysis reveals that pardaxin triggers apoptotic signaling pathways in human cervical carcinoma HeLa cells: cross talk among the UPR, c-Jun and ROS.
AuthorsHuang TC, Chen JY,
JournalCarcinogenesis
PubMed ID23615400
Pardaxin, an antimicrobial peptide secreted by the Red Sea flatfish Pardachirus marmoratus, inhibits proliferation and induces apoptosis of human cancer cell lines. However, the underlying molecular mechanisms are only partially understood at present. In this study, we used proteomic approaches and network reconstruction to clarify the mechanism of pardaxin-induced apoptosis ... More
Salinomycin inhibits breast cancer progression via targeting HIF-1a/VEGF mediated tumor angiogenesis in vitro and in vivo.
AuthorsDewangan J, Srivastava S, Mishra S, Divakar A, Kumar S, Rath SK
JournalBiochem Pharmacol
PubMed ID31028743
'Cancer is a complex disease wherein cells begin to divideabnormally and spread into surrounding tissues. Angiogenesis plays a crucial role in tumor progression as it is required for sustained growth and metastasis, therefore targeting angiogenesis is a promising therapeutic approach for breast cancer management. Salinomycin (SAL) has been reported to ... More
Gel-based cell manipulation method for isolation and genotyping of single-adherent cells.
AuthorsNegishi R, Iwata R, Tanaka T, Kisailus D, Maeda Y, Matsunaga T, Yoshino T
JournalAnalyst
PubMed ID30302469
'Genetic analysis of single-cells is widely recognized as a powerful tool for understanding cellular heterogeneity and obtaining genetic information from rare populations. Recently, many kinds of single-cell isolation systems have been developed to facilitate single-cell genetic analysis. However, these systems mainly target non-adherent cells or cells in a cell suspension. ... More
Clumping and Viability of Bone Marrow Derived Mesenchymal Stromal Cells under Different Preparation Procedures: A Flow Cytometry-Based In Vitro Study.
'Complications of microocclusions have been reported after intra-arterial delivery of mesenchymal stromal cells. Hence, quantification and efficient limitation of cell clumps in suspension before transplantation is important to reduce the risk. We used a flow cytometry-based pulse-width assay to assess the effects of different cell suspension concentrations (0.2-2.0 × 10(6)/mL), ... More
Avenanthramide-C reduces the viability of MDA-MB-231 breast cancer cells through an apoptotic mechanism.
AuthorsHastings J, Kenealey J
JournalCancer Cell Int
PubMed ID29075150
Avenanthramides (AVN) are a relatively unstudied family of phytochemicals that could be novel chemotherapeutics. These compounds, found in oats, are non-toxic to healthy cells and have been shown to reduce viability of human colon and liver cancers in vitro. However, these studies do not elucidate a molecular mechanism for individual ... More
Effect of mTHPC-mediated photodynamic therapy on 5-fluorouracil resistant human colorectal cancer cells.
AuthorsKukcinaviciute E, Sasnauskiene A, Dabkeviciene D, Kirveliene V, Jonusiene V
JournalPhotochem Photobiol Sci
PubMed ID28509917
The primary or acquired cancer chemoresistance is a major problem in the treatment of cancer patients. It could be overcome by non-overlapping treatment regimens such as photodynamic therapy (PDT). PDT is based on the oxidation of cellular components which occurs when a light-excited photosensitizer generates reactive oxygen species (ROS). In ... More
Novel combination of salinomycin and resveratrol synergistically enhances the anti-proliferative and pro-apoptotic effects on human breast cancer cells.
AuthorsDewangan J, Tandon D, Srivastava S, Verma AK, Yapuri A, Rath SK
JournalApoptosis
PubMed ID28748373
Resveratrol (RES) is a natural polyphenol having anti-proliferative activity against breast cancer cells. RES in combination with other chemo modulatory agents, minimizes toxicity and increases efficacy of the treatment. Salinomycin (SAL), a monocarboxylic polyether ionophore is known for selectively targeting breast cancer stem cells. Purpose of the present study was ... More
Andrographolide simultaneously augments Nrf2 antioxidant defense and facilitates autophagic flux blockade in cigarette smoke-exposed human bronchial epithelial cells.
AuthorsTan WSD, Liao W, Peh HY, Vila M, Dong J, Shen HM, Wong WSF
JournalToxicol Appl Pharmacol
PubMed ID30291937
Cigarette smoking is the leading cause of chronic obstructive pulmonary disease (COPD). Cigarette smoke heightens oxidative stress and impairs autophagy, advancing COPD progression. Andrographolide is a bioactive diterpenoid lactone isolated from the plant Andrographis paniculata which has been a traditional medicinal herb for respiratory diseases. As airway epithelial cells form ... More
SNHG6 is upregulated in primary breast cancers and promotes cell cycle progression in breast cancer-derived cell lines.
AuthorsJafari-Oliayi A, Asadi MH
JournalCell Oncol (Dordr)
PubMed ID30826970
Long non-coding RNAs (lncRNAs) are known as RNAs that do not encode proteins and that are more than 200 nucleotides in size. Previously, it has been found that LncRNAs play crucial roles in normal cellular processes, including proliferation and apoptosis. A growing body of evidence suggests that lncRNAs may also ... More
The HERV-K accessory protein Np9 controls viability and migration of teratocarcinoma cells.
AuthorsChan SM, Sapir T, Park SS, Rual JF, Contreras-Galindo R, Reiner O, Markovitz DM
JournalPLoS One
PubMed ID30818388
Human endogenous retroviruses are remnants of ancient germline infections that make up approximately 8% of the modern human genome. The HERV-K (HML-2) family is one of the most recent entrants into the human germline, these viruses appear to be transcriptionally active, and HERV-K viral like particles (VLPs) are found in ... More
Effects of the cryopreservation process on dog sperm integrity.
AuthorsSicherle CC, de Souza FF, Freitas-Dell'Aqua CP, Mothé GB, Padovani CR, Papa FO, Lopes MD
JournalAnim Reprod
PubMed ID32399067
Sperm cryopreservation has become an indispensable tool in reproductive biology. However, frozen/thawed semen has a short lifespan due to loss of sperm cell integrity. To better understand which sperm cell structures are compromised by the cryopreservation process and apoptosis markers, the sperm of five healthy mature dogs was analyzed in ... More