Arsenic increases Pi-mediated vascular calcification and induces premature senescence in vascular smooth muscle cells.
AuthorsMartÃn-Pardillos A, Sosa C, Sorribas V,
JournalToxicol Sci
PubMed ID23104429
Several mechanisms have been proposed to explain the vascular toxicity of arsenic. Some of them are described in this work, such as stress-induced premature senescence (SIPS), dedifferentiation, and medial vascular calcification, and they all affect vascular smooth muscle cells (VSMC). Rat aortic VSMC were treated with 1-100 µM of either ... More
AuthorsSollinger D, Eißler R, Lorenz S, Strand S, Chmielewski S, Aoqui C, Schmaderer C, Bluyssen H, Zicha J, Witzke O, Scherer E, Lutz J, Heemann U, Baumann M,
Journal
PubMed ID24302630
'Recent publications have shed new light on the role of the adaptive and innate immune system in the pathogenesis of hypertension. However, there are limited data whether receptors of the innate immune system may influence blood pressure. Toll-like receptor 4 (TLR4), a pattern recognition receptor, is a key component of ... More
Sigma receptor ligand, (+)-pentazocine, suppresses inflammatory responses of retinal microglia.
AuthorsZhao J, Ha Y, Liou GI, Gonsalvez GB, Smith SB, Bollinger KE,
Journal
PubMed ID24812552
'To evaluate the effects of the s 1 receptor (sR1) agonist, (+)-pentazocine, on lipopolysaccharide (LPS)-induced inflammatory changes in retinal microglia cells. Retinal microglia cells were isolated from Sprague-Dawley rat pups. Cells were treated with LPS with or without (+)-pentazocine and with or without the sR1 antagonist BD1063. Morphologic changes were ... More
Ubiquilin-1 protects cells from oxidative stress and ischemic stroke caused tissue injury in mice.
AuthorsLiu Y, Lü L, Hettinger CL, Dong G, Zhang D, Rezvani K, Wang X, Wang H,
Journal
PubMed ID24553923
Ubiquilin-1 (Ubqln1 or Ubqln), a ubiquitin-like protein, mediates degradation of misfolded proteins and has been implicated in a number of pathological and physiological conditions. To better understand its function in vivo, we recently generated transgenic (Tg) mice that globally overexpress mouse Ubqln in a variety of tissues and ubqln conditional ... More
Loss of the m-AAA protease subunit AFG3L2 causes mitochondrial transport defects and tau hyperphosphorylation.
AuthorsKondadi AK, Wang S, Montagner S, Kladt N, Korwitz A, Martinelli P, Herholz D, Baker MJ, Schauss AC, Langer T, Rugarli EI,
Journal
PubMed ID24681487
The m-AAA protease subunit AFG3L2 is involved in degradation and processing of substrates in the inner mitochondrial membrane. Mutations in AFG3L2 are associated with spinocerebellar ataxia SCA28 in humans and impair axonal development and neuronal survival in mice. The loss of AFG3L2 causes fragmentation of the mitochondrial network. However, the ... More
Interleukin-6 Is Required for Pancreatic Cancer Progression by Promoting MAPK Signaling Activation and Oxidative Stress Resistance.
AuthorsZhang Y, Yan W, Collins MA, Bednar F, Rakshit S, Zetter BR, Stanger BZ, Chung I, Rhim AD, di Magliano MP,
Journal
PubMed ID24097820
Pancreatic cancer, one of the deadliest human malignancies, is almost invariably associated with the presence of an oncogenic form of Kras. Mice expressing oncogenic Kras in the pancreas recapitulate the stepwise progression of the human disease. The inflammatory cytokine interleukin (IL)-6 is often expressed by multiple cell types within the ... More
Cysteamine Modulates Oxidative Stress and Blocks Myofibroblast Activity in CKD.
Therapy to slow the relentless expansion of interstitial extracellular matrix that leads to renal functional decline in patients with CKD is currently lacking. Because chronic kidney injury increases tissue oxidative stress, we evaluated the antifibrotic efficacy of cysteamine bitartrate, an antioxidant therapy for patients with nephropathic cystinosis, in a mouse ... More
Dynamics of sub-lethal effects of nano-CuO on the microalga Chlamydomonas reinhardtii during short-term exposure.
Authorsvon Moos N, Maillard L, Slaveykova VI,
Journal
PubMed ID25731685
Though nano-CuO has been classified as toxic toward aquatic microorganisms and its use in various applications is expected to increase in near future, its ecotoxicity is currently poorly understood. The aim of this study was to investigate the mechanisms of nano-CuO toxicity based on the paradigm of oxidative stress, the ... More
Highly specific in vivo gene delivery for p53-mediated apoptosis and genetic photodynamic therapies of tumour.
AuthorsTseng SJ, Liao ZX, Kao SH, Zeng YF, Huang KY, Li HJ, Yang CL, Deng YF, Huang CF, Yang SC, Yang PC, Kempson IM,
Journal
PubMed ID25739372
Anticancer therapies are often compromised by nonspecific effects and challenged by tumour environments' inherent physicochemical and biological characteristics. Often, therapeutic effect can be increased by addressing multiple parameters simultaneously. Here we report on exploiting extravasation due to inherent vascular leakiness for the delivery of a pH-sensitive polymer carrier. Tumours' acidic ... More
A tryptophanol-derived oxazolopiperidone lactam is cytotoxic against tumors via inhibition of p53 interaction with murine double minute proteins.
AuthorsSoares J, Raimundo L, Pereira NA, Dos Santos DJ, Pérez M, Queiroz G, Leão M, Santos MM, Saraiva L,
Journal
PubMed ID25814188
Inactivation of the p53 tumor suppressor protein by interaction with murine double minute (MDM) proteins, MDM2 and MDMX, is a common event in human tumors expressing wild-type p53. In these tumors, the simultaneous inhibition of these interactions with MDMs, for a full p53 reactivation, represents a promising anticancer strategy. Herein, ... More
Iron Homeostasis and Pulmonary Hypertension: Iron Deficiency Leads to Pulmonary Vascular Remodeling in the Rat.
AuthorsCotroneo E, Ashek A, Wang L, Wharton J, Dubois OD, Bozorgi SS, Busbridge M, Alavian KN, Wilkins MR, Zhao L,
Journal
PubMed ID25767292
Iron deficiency without anaemia is prevalent in patients with idiopathic pulmonary arterial hypertension and associated with reduced exercise capacity and survival. We hypothesised that iron deficiency is involved in the pathogenesis of pulmonary hypertension and iron replacement is a possible therapeutic strategy. Rats were fed an iron-deficient diet (IDD, 7mg/kg) ... More
Antioxidant enzymes mediate survival of breast cancer cells deprived of extracellular matrix.
AuthorsDavison CA, Durbin SM, Thau MR, Zellmer VR, Chapman SE, Diener J, Wathen C, Leevy WM, Schafer ZT,
Journal
PubMed ID23771908
Metastasis by cancer cells relies upon the acquisition of the ability to evade anoikis, a cell death process elicited by detachment from extracellular matrix (ECM). The molecular mechanisms that ECM-detached cancer cells use to survive are not understood. Striking increases in reactive oxygen species (ROS) occur in ECM-detached mammary epithelial ... More
Biologically Active Metabolites Produced by the Basidiomycete Quambalaria cyanescens.
AuthorsStodulková E, Císarová I, Kolarík M, Chudícková M, Novák P, Man P, Kuzma M, Pavlu B, Cerný J, Flieger M,
Journal
PubMed ID25723150
Four strains of the fungus Quambalaria cyanescens (Basidiomycota: Microstromatales), were used for the determination of secondary metabolites production and their antimicrobial and biological activities. A new naphthoquinone named quambalarine A, (S)-(+)-3-(5-ethyl-tetrahydrofuran-2-yliden)-5,7,8-trihydroxy-2-oxo-1,4-naphthoquinone (1), together with two known naphthoquinones, 3-hexanoyl-2,5,7,8-tetrahydroxy-1,4-naphthoquinone (named here as quambalarine B, 2) and mompain, 2,5,7,8-tetrahydroxy-1,4-naphthoquinone (3) were isolated. ... More
The mechanisms by which pardaxin, a natural cationic antimicrobial peptide, targets the endoplasmic reticulum and induces c-FOS.
Pardaxin is a cationic antimicrobial peptide derived from Red Sea Moses sole. Previous studies have shown that pardaxin selectively triggers the death of cancer cells, initiating the development of a pardaxin-based cancer vaccine; however, the underlying mechanism by which pardaxin kills cancer cells has not yet been elucidated. Here, we ... More
Lymph protects metastasizing melanoma cells from ferroptosis.
Authors
JournalNature
PubMed ID32814895
Short-term urea cycle inhibition in rat liver cells induced by polyethylene glycol.
AuthorsXu L, Yang J, Liu Y, Shi L, Wu C, Jin H, Jin X, Su Y, Zhu X
JournalBiomater Sci
PubMed ID30209472
'Polyethylene glycol (PEG) is widely used in the biomedical field due to its outstanding properties. There are plenty of reports on the safety of PEG, but they are mostly restricted to its pharmacokinetic behaviour and pathological effect in vivo, and fail to elucidate its biological effects on cells at the ... More
Molecular Imaging of Factor XIII Activity for the Early Detection of Mouse Coronary Microvascular Disease.
AuthorsZhuang ZW, Huang Y, Ju R, Maxfield MW, Ren Y, Wang X, Wang X, Stacy MR, Hwa J
JournalTheranostics
PubMed ID30867844
'Coronary microvascular disease (MVD) remains a major clinical problem due to limited mechanistic understanding and a challenging diagnosis. In the present study we evaluated the utility of targeted imaging of active factor XIII (FXIII) for detection of coronary MVD associated with thrombus. We hypothesized that a high specificity and sensitivity ... More
Molecular insights for the biological interactions between polyethylene glycol and cells.
AuthorsXu L, Yang J, Xue B, Zhang C, Shi L, Wu C, Su Y, Jin X, Liu Y, Zhu X
JournalBiomaterials
PubMed ID28923681
'As the gold standard polymer for drug delivery system, polyethylene glycol (PEG) has excellent biocompatibility. It''s reported that the low nonspecific interactions between PEG and body contribute to its biocompatibility. However, here we discover dynamic biological interactions exist between PEG and cells on the molecular level. PEG (2 kD) can ... More
A non-dividing cell population with high pyruvate dehydrogenase kinase activity regulates metabolic heterogeneity and tumorigenesis in the intestine.
Authors
JournalNat Commun
PubMed ID35314684
Single-Cell, Time-Lapse Reactive Oxygen Species Detection in E. coli.
AuthorsYang Z, Choi H
JournalCurr Protoc Cell Biol
PubMed ID30028910
'Detection of reactive oxygen species (ROS) in bacteria has been limited to bulk biochemical assays. Although they are powerful and quantitative tools to understand the overall production of ROS in E. coli, such assays provide limited spatial and temporal information when correlating cellular phenotype with perturbations such as antibiotics or ... More
Diphthamide affects selenoprotein expression: Diphthamide deficiency reduces selenocysteine incorporation, decreases selenite sensitivity and pre-disposes to oxidative stress.
AuthorsMayer K, Mundigl O, Kettenberger H, Birzele F, Stahl S, Pastan I, Brinkmann U
JournalRedox Biol
PubMed ID30312900
'The diphthamide modification of translation elongation factor 2 is highly conserved in eukaryotes and archaebacteria. Nevertheless, cells lacking diphthamide can carry out protein synthesis and are viable. We have analyzed the phenotypes of diphthamide deficient cells and found that diphthamide deficiency reduces selenocysteine incorporation into selenoproteins. Additional phenotypes resulting from diphthamide deficiency ... More
Generation of Human Fatty Livers Using Custom-Engineered Induced Pluripotent Stem Cells with Modifiable SIRT1 Metabolism.
AuthorsNiture S, Gyamfi MA, Lin M, Chimeh U, Dong X, Zheng W, Moore J, Kumar D
JournalCell Death Dis
PubMed ID32152268
'Tumor necrosis factor-a-induced protein 8 (TNFAIP8) expression has been linked to tumor progression in various cancer types, but the detailed mechanisms of TNFAIP8 are not fully elucidated. Here we define the role of TNFAIP8 in early events associated with development of hepatocellular carcinoma (HCC). Increased TNFAIP8 levels in HCC cells ... More
Oxidative Metabolism Drives Immortalization of Neural Stem Cells during Tumorigenesis.
Authors
JournalCell
PubMed ID32916131
Arginine is an epigenetic regulator targeting TEAD4 to modulate OXPHOS in prostate cancer cells.
Authors
JournalNat Commun
PubMed ID33893278
Non-canonical Glutamate-Cysteine Ligase Activity Protects against Ferroptosis.
Authors
JournalCell Metab
PubMed ID33357455
Discretizing Three-Dimensional Oxygen Gradients to Modulate and Investigate Cellular Processes.
Fabrication of Smart Tantalum Carbide MXene Quantum Dots with Intrinsic Immunomodulatory Properties for Treatment of Allograft Vasculopathy.
Authors
JournalAdv Funct Mater
PubMed ID35153642
iPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications.
Authors
JournalCell
PubMed ID29576452
Ferroptosis Contributes to Isoflurane Neurotoxicity.
AuthorsXia Y, Sun X, Luo Y, Stary CM
JournalFront Mol Neurosci
PubMed ID30687003
The underlying mechanisms of isoflurane neurotoxicity in the developing brain remain unclear. Ferroptosis is a recently characterized form of programmed cell death distinct from apoptosis or autophagy, characterized by iron-dependent reactive oxygen species (ROS) generation secondary to failure of glutathione-dependent antioxidant defenses. The results of the present study are the ... More
p53-mediated adaptation to serine starvation is retained by a common tumour-derived mutant.
In response to oncogenic stress, the tumour suppressor protein p53 can induce the elimination of cells through induction of cell death or senescence, helping to restrain malignant progression. Conversely, under nutrient stress, p53 can protect cells by supporting metabolic adaptation. Many cancers express mutant p53 proteins that have lost the ... More
Impaired function of aorta and perivascular adipose tissue in IL-18-deficient mice.
AuthorsLi W, Jin D, Takai S, Hayakawa T, Ogata J, Yamanishi K, Yamanishi H, Okamura H
JournalAm J Physiol Heart Circ Physiol
PubMed ID31518161
IL-18 is ubiquitously produced by both hematopoietic and non-hematopoietic cells. The present study examined the thoracic aorta, including the surrounding perivascular adipose tissue (PVAT), of IL-18KO mice from functional and histological perspectives. IL-18KO mice exhibited raised blood pressure compared with wild-type mice. Echocardiographic examination showed a thickened vascular wall and ... More
Dual role of oxidative stress-JNK activation in autophagy and apoptosis induced by nickel oxide nanoparticles in human cancer cells.
AuthorsCho YL, Tan HWS, Saquib Q, Ren Y, Ahmad J, Wahab R, He W, Bay BH, Shen HM
JournalFree Radic Biol Med
PubMed ID32353482
Nickel oxide nanoparticles (NiO-NPs) are an important group of nanoparticles with increasing applications in many aspects of industry. At present, there is evidence demonstrating the cytotoxic characteristics of NiO-NPs, while the involvement of autophagy in the cytotoxicity of NiO-NPs has not been reported. In this study, we aimed to study ... More