Immuno-spin trapping of heme-induced protein radicals: Implications for heme oxygenase-1 induction and heme degradation.
AuthorsKumar A, Ganini D, Deterding LJ, Ehrenshaft M, Chatterjee S, Mason RP
JournalFree Radic Biol Med
PubMed ID23624303
'Heme, in the presence of hydrogen peroxide, can act as a peroxidase. Intravascular hemolysis results in a massive release of heme into the plasma in several pathophysiological conditions such as hemolytic anemia, malaria, and sickle cell disease. Heme is known to induce heme oxygenase-1(HO-1) expression, and the extent of induction ... More
Cadmium disorganises the scaffolding of gap and tight junction proteins in the hepatic cell line WIF B9.
AuthorsBoucherie S, Decaens C, Verbavatz JM, Grosse B, Erard M, Merola F, Cassio D, Combettes L
Journal
PubMed ID24117459
'Hepatocytes, which perform the main functions of the liver, are particularly vulnerable to toxic agents such as cadmium, an environmental pollutant. To identify the molecular targets for cadmium in hepatocytes, we have studied the effects of CdCl2 on the hybrid cell line WIF-B9 that exhibits stable structural and functional hepatocytic ... More
Use of fluorogenic probes to differentiate between hydrophilic and lipophilic antioxidant activity in a fish cell line.
AuthorsBain PA, Schuller KA,
JournalJ Agric Food Chem
PubMed ID22136585
'In finfish aquaculture, dietary antioxidants have been shown to improve indicators of general fish health and to inhibit the oxidative deterioration of polyunsaturated fatty acids. To facilitate the characterization of novel antioxidants or antioxidant mixtures, we developed assays for antioxidant activity in a fish cell line. We used 2'',7''-dichlorodihydrofluorescein diacetate ... More
Temperature acclimation alters oxidative capacities and composition of membrane lipids without influencing activities of enzymatic antioxidants or susceptibility to lipid peroxidation in fish muscle.
AuthorsGrim JM, Miles DR, Crockett EL,
JournalJ Exp Biol
PubMed ID20086129
'Cold acclimation of ectotherms results typically in enhanced oxidative capacities and lipid remodeling, changes that should increase the risk of lipid peroxidation (LPO). It is unclear whether activities of antioxidant enzymes may respond in a manner to mitigate the increased potential for LPO. The current study addresses these questions using ... More
Supra-physiological doses of testosterone affect membrane oxidation of human neutrophils monitored by the fluorescent probe C(11)-BODIPY (581/591).
'The purpose of this study was to determine the effects of supra-physiological doses of testosterone (TES) on membrane oxidation of activated human neutrophils in vitro using an innovative and sensitive technique: the real-time detection with the fluorescence probe C(11)-BODIPY(581/591). Methodological controls were performed with the lipid-soluble and powerful antioxidant astaxanthin ... More
Dopamine induces Ca2+ signaling in astrocytes through reactive oxygen species generated by monoamine oxidase.
AuthorsVaarmann A, Gandhi S, Abramov AY,
JournalJ Biol Chem
PubMed ID20547771
'Dopamine is a neurotransmitter that plays a major role in a variety of brain functions, as well as in disorders such as Parkinson disease and schizophrenia. In cultured astrocytes, we have found that dopamine induces sporadic cytoplasmic calcium ([Ca(2+)](c)) signals. Importantly, we show that the dopamine-induced calcium signaling is receptor-independent ... More
Surface-localized spermidine protects the Pseudomonas aeruginosa outer membrane from antibiotic treatment and oxidative stress.
AuthorsJohnson L, Mulcahy H, Kanevets U, Shi Y, Lewenza S,
JournalJ Bacteriol
PubMed ID22155771
'Extracellular DNA acts as a cation chelator and induces the expression of antibiotic resistance genes regulated by Mg(2+) levels. Here we report the characterization of novel DNA-induced genes in Pseudomonas aeruginosa that are annotated as homologs of the spermidine synthesis genes speD (PA4773) and speE (PA4774). The addition of sublethal ... More
Adding insult to injury: effects of xenobiotic-induced preantral ovotoxicity on ovarian development and oocyte fusibility.
AuthorsSobinoff AP, Pye V, Nixon B, Roman SD, McLaughlin EA,
JournalToxicol Sci
PubMed ID20829426
Mammalian females are born with a finite number of nonrenewing primordial follicles, the majority of which remain in a quiescent state for many years. Because of their nonrenewing nature, these ... More
Detection of lipid peroxidation in frozen-thawed avian spermatozoa using C(11)-BODIPY(581/591).
AuthorsPartyka A, Lukaszewicz E, Nizanski W, Twardon J,
JournalTheriogenology
PubMed ID21396693
The aim of this study was to perform flow cytometric analysis of C11-BODIPY581/591 oxidation in fowl and geese sperm as a marker for membrane lipid peroxidation (LPO) and to establish if the cryopreservation process would make sperm membranes more susceptible to oxidative stress. The experiment was carried out on 10 ... More
Protective effects of in vitro treatment with zinc, d-aspartate and coenzyme q10 on human sperm motility, lipid peroxidation and DNA fragmentation.
AuthorsTalevi R, Barbato V, Fiorentino I, Braun S, Longobardi S, Gualtieri R
Journal
PubMed ID23958080
Spermatozoa are extremely vulnerable to oxidative stress caused by the unbalance between concentrations of reactive oxygen species and antioxidant scavenging systems present inside the male reproductive tract. In spite of a large number of clinical studies that claimed the beneficial effects of antioxidant oral administration on sperm physiology and fertility, ... More
AuthorsThevenot PT, Saravia J, Jin N, Giaimo JD, Chustz RE, Mahne S, Kelley MA, Hebert VY, Dellinger B, Dugas TR, Demayo FJ, Cormier SA,
JournalAm J Respir Cell Mol Biol
PubMed ID23087054
Environmentally persistent free radicals (EPFRs) in combustion-generated particulate matter (PM) are capable of inducing pulmonary pathologies and contributing to the development of environmental asthma. In vivo exposure of infant rats to EPFRs demonstrates their ability to induce airway hyperresponsiveness to methacholine, a hallmark of asthma. However, the mechanisms by which ... More
Prostaglandin H synthase-2-catalyzed oxygenation of 2-arachidonoylglycerol is more sensitive to peroxide tone than oxygenation of arachidonic acid.
AuthorsMusee J, Marnett LJ,
JournalJ Biol Chem
PubMed ID22942274
The endocannabinoid, 2-arachidonoylglycerol (2-AG), is a selective substrate for the inducible isoform of prostaglandin H synthase (PGHS), PGHS-2. Its turnover leads to the formation of glyceryl esters of prostaglandins (PG-Gs), a subset of which elicits agonism at unique, as yet unidentified, receptors. The k(cat)/K(m) values for oxygenation of arachidonic acid ... More
Development of a novel micro-assay for evaluation of peroxyl radical scavenger capacity: application to carotenoids and structure-activity relationship.
AuthorsRodrigues E, Mariutti LR, Chisté RC, Mercadante AZ,
JournalFood Chem
PubMed ID22953962
A micro-assay was developed and validated, using a microplate reader in 96-well format, C(11)-BODIPY(581/591) as fluorescent probe and AIBN as ROO() generator. The structure-activity relationship was established for 15 carotenoid standards, indicating that the opening of the ß-ionone ring and the increase of chromophore extension in the carotenoid structure were ... More
Inter-relationship between mitochondrial function and susceptibility to oxidative stress in red- and white-blooded Antarctic notothenioid fishes.
AuthorsMueller IA, Grim JM, Beers JM, Crockett EL, O'Brien KM,
JournalJ Exp Biol
PubMed ID22031737
It is unknown whether Antarctic fishes can defend themselves against oxidative stress induced by elevations in temperature. We hypothesized that Antarctic icefishes, lacking the oxygen-binding protein hemoglobin, might be more vulnerable to temperature-induced oxidative stress compared with red-blooded notothenioids because of differences in their mitochondrial properties. Mitochondria from icefishes have ... More
AuthorsCho SC, Park MC, Keam B, Choi JM, Cho Y, Hyun S, Park SC, Lee J,
JournalProc Natl Acad Sci U S A
PubMed ID20974969
DDS, 4,4'-diaminodiphenylsulfone, is the most common drug prescribed to treat Hansen disease patients. In addition to its antibacterial activity, DDS has been reported to be involved in other cellular processes that occur in eukaryotic cells. Because DDS treatment significantly enhances the antioxidant activity in humans, we examined its effect on ... More
Comparison of the TBARS assay and BODIPY C11 probes for assessing lipid peroxidation in red deer spermatozoa.
AuthorsDomínguez-Rebolledo ÁE, Martínez-Pastor F, Fernández-Santos MR, del Olmo E, Bisbal A, Ros-Santaella JL, Garde JJ,
JournalReprod Domest Anim
PubMed ID20113445
Several methods are used to measure lipid peroxidation (LPO) in spermatozoa. The objective of this study was comparing the thiobarbituric acid reactive species (TBARS) method and the BODIPY 581/591 C(11) (B581) and BODIPY 665/676 C(11) (B665) fluorescent probes to measure induced peroxidative damage in thawed epididymal spermatozoa from Iberian red ... More
Using fluorescence-activated flow cytometry to determine reactive oxygen species formation and membrane lipid peroxidation in viable boar spermatozoa.
AuthorsGuthrie HD, Welch GR,
JournalMethods Mol Biol
PubMed ID20072917
Fluorescence-activated flow cytometry analyses were developed for determination of reactive oxygen species (ROS) formation and membrane lipid peroxidation in live spermatozoa loaded with, respectively, hydroethidine (HE) or the lipophilic probe 4,4-difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid, C(11)BODIPY(581/591) (BODIPY). ROS was detected by red fluorescence emission from oxidization of HE and membrane lipid peroxidation was ... More
Bromoalkaloids protect primary cortical neurons from induced oxidative stress.
AuthorsLeirós M, Alonso E, Rateb ME, Houssen WE, Ebel R, Jaspars M, Alfonso A, Botana LM
Journal
PubMed ID25387680
Bromoalkaloids are secondary metabolites with a demonstrated high activity in several therapeutic areas. In this research, we probe the neuroprotective and antioxidant activities of hymenialdisine and hymenin. Both structures were tested in an oxidative stress cellular model, consisting of cortical neurons that are incubated with the oxidative stress inducer hydrogen ... More
Role of membrane cholesterol in spontaneous exocytosis at frog neuromuscular synapses: reactive oxygen species-calcium interplay.
AuthorsPetrov AM, Yakovleva AA, Zefirov AL
Journal
PubMed ID25326454
Using electrophysiological and optical techniques, we studied the mechanisms by which cholesterol depletion stimulates spontaneous transmitter release by exocytosis at the frog neuromuscular junction. We found that methyl-ß-cyclodextrin (MCD, 10 mM)-mediated exhaustion of cholesterol resulted in the enhancement of reactive oxygen species (ROS) production, which was prevented by the antioxidant ... More
The lipophilic vitamin C derivative, 6-o-palmitoylascorbate, protects human lymphocytes, preferentially over ascorbate, against X-ray-induced DNA damage, lipid peroxidation, and protein carbonylation.
AuthorsXiao L, Tsutsui T, Miwa N
Journal
PubMed ID24898782
The aim of this study was to investigate protective effects of the lipophilic vitamin C derivative, 6-o-palmitoylascorbate (PlmtVC), against X-ray radiation-induced damages including cell death, DNA double-strand breaks (DSBs), lipid peroxidation, and protein carbonylation in human lymphocytes HEV0082, and the stability of PlmtVC under cell-cultured or cell-free condition. Irradiation with ... More
Potent and specific antitumor effect of CEA-targeted photoimmunotherapy.
AuthorsShirasu N, Yamada H, Shibaguchi H, Kuroki M, Kuroki M
Journal
PubMed ID24740257
Conventional photodynamic therapy (PDT) for cancer is limited by the insufficient efficacy and specificity of photosensitizers. We herein describe a highly effective and selective tumor-targeted PDT using a near-infrared (NIR) photosensitizer, IRDye700DX, conjugated to a human monoclonal antibody (Ab) specific for carcinoembryonic antigen (CEA). The antitumor effects of this Ab-assisted ... More
Fungal lignin peroxidase does not produce the veratryl alcohol cation radical as a diffusible ligninolytic oxidant.
AuthorsHoutman CJ, Maligaspe E, Hunt CG, Fernández-Fueyo E, Martínez AT, Hammel KE
JournalJ Biol Chem
PubMed ID29462790
'Peroxidases are considered essential agents of lignin degradation by white-rot basidiomycetes. However, low-molecular-weight oxidants likely have a primary role in lignin breakdown because many of these fungi delignify wood before its porosity has sufficiently increased for enzymes to infiltrate. It has been proposed that lignin peroxidases (LPs, EC 1.11.1.14) fulfill ... More
Analysis of Autophagy for Liver Pathogenesis.
AuthorsHuda N, Zou H, Yan S, Khambu B, Yin XM
JournalMethods Mol Biol
PubMed ID30610716
'The autophagy pathway in hepatocytes is well characterized. Autophagy plays a critical role in the normal function of the liver. A growing number of studies suggest that there is a mechanistic relationship between autophagy and the pathogenesis of human diseases including liver diseases. Here we focus on the methods assessing ... More
Abrogation of ARF6 promotes RSL3-induced ferroptosis and mitigates gemcitabine resistance in pancreatic cancer cells.
AuthorsYe Z, Hu Q, Zhuo Q, Zhu Y, Fan G, Liu M, Sun Q, Zhang Z, Liu W, Xu W, Ji S, Yu X, Xu X, Qin Y
JournalAm J Cancer Res
PubMed ID32368394
'ADP Ribosylation Factor 6 (ARF6) is a part of the RAS superfamily and regulates vesicular trafficking, remodeling of membrane lipids, and signaling pathways. Our previous study has found that ARF6, functioned as a downstream of Kras/ERK signaling pathway, could promote proliferation and Warburg effect in pancreatic cancer cells. Moreover, ARF6 ... More
Peroxiredoxin-1 regulates lipid peroxidation in corneal endothelial cells.
AuthorsLovatt M, Adnan K, Kocaba V, Dirisamer M, Peh GSL, Mehta JS
JournalRedox Biol
PubMed ID31901729
'Corneal transparency is maintained by a monolayer of corneal endothelial cells. Defects in corneal endothelial cells (CEnCs) can be rectified surgically through transplantation. Fuchs'' endothelial corneal dystrophy (FECD) is the foremost cause of endothelial dysfunction and the leading indication for transplantation. Increased sensitivity of CEnCs to oxidative stress is thought ... More
Selective covalent targeting of GPX4 using masked nitrile-oxide electrophiles.
Authors
JournalNat Chem Biol
PubMed ID32231343
p53-mediated redox control promotes liver regeneration and maintains liver function in response to CCl4.
Authors
JournalCell Death Differ
PubMed ID34628485
Malic Enzyme 1 Absence in Synovial Sarcoma Shifts Antioxidant System Dependence and Increases Sensitivity to Ferroptosis Induction with ACXT3102.
Authors
JournalClin Cancer Res
PubMed ID35421237
Lipid peroxidation regulates long-range wound detection through 5-lipoxygenase in zebrafish.
Authors
JournalNat Cell Biol
PubMed ID32868902
Cysteine dioxygenase 1 is a metabolic liability for non-small cell lung cancer.
Authors
JournalElife
PubMed ID31107239
Carbomer-based adjuvant elicits CD8 T-cell immunity by inducing a distinct metabolic state in cross-presenting dendritic cells.
Authors
JournalPLoS Pathog
PubMed ID33444400
Non-canonical Glutamate-Cysteine Ligase Activity Protects against Ferroptosis.
Authors
JournalCell Metab
PubMed ID33357455
Live Imaging of Label-Free Graphene Oxide Reveals Critical Factors Causing Oxidative-Stress-Mediated Cellular Responses.
Authors
JournalACS Nano
PubMed ID29286639
Biophysical experiments reveal a protective role of protein phosphatase Z1 against oxidative damage of the cell membrane in Candida albicans.
Authors
JournalFree Radic Biol Med
PubMed ID34582996
Cellular metabolism and oxidative stress as a possible determinant for longevity in small breed and large breed dogs.
AuthorsJimenez AG, Winward J, Beattie U, Cipolli W
JournalPLoS One
PubMed ID29694441
Among species, larger animals tend to live longer than smaller ones, however, the opposite seems to be true for dogs-smaller dogs tend to live significantly longer than larger dogs across all breeds. We were interested in the mechanism that may allow for small breeds to age more slowly compared with ... More
Age-Related Oxidative Changes in Primary Porcine Fibroblasts Expressing Mutated Huntingtin.
AuthorsSmatlikova P, Askeland G, Vaskovicova M, Klima J, Motlik J, Eide L, Ellederová Z
JournalNeurodegener Dis
PubMed ID31167196
Huntington's disease (HD) is a devastating neurodegenerative disorder caused by CAG triplet expansions in the huntingtin gene. Oxidative stress is linked to HD pathology, although it is not clear whether this is an effect or a mediator of disease. The transgenic (TgHD) minipig expresses the N-terminal part of human-mutated huntingtin ... More
The oncometabolite 2-hydroxyglutarate produced by mutant IDH1 sensitizes cells to ferroptosis.
Ferroptosis is a non-apoptotic form of cell death characterized by the iron-dependent lipid peroxidation and is implicated in several human pathologies, such as tissue ischemia, neurodegeneration, and cancer. Ferroptosis appears to be high cell-context dependent and the regulation of ferroptosis by physiological or pathological conditions are unclear. Here, we report ... More
Effect of gentisic acid on the structural-functional properties of liposomes incorporating ß-sitosterol.
AuthorsJovanovic AA, Balanc BD, Djordjevic VB, Ota A, Skrt M, Šavikin KP, Bugarski BM, Nedovic VA, Ulrih NP
JournalColloids Surf B Biointerfaces
PubMed ID31437609
Multifunctional liposomes incorporating ß-sitosterol were developed for delivery of gentisic acid (GA). The interactions of both compounds with phospholipid bilayer were interpreted viaeffects of different ß-sitosterol content (0, 20 and 50?mol %) and different gentisic acid to lipid ratio (n ... More