CellROX™ 染料试剂包(适用于氧化应激检测)
CellROX™ 染料试剂包(适用于氧化应激检测)
Invitrogen™

CellROX™ 染料试剂包(适用于氧化应激检测)

CellROX™ 一件混合装包含 CellROX™ Green 试剂、CellROX™ Orange 试剂和 CellROX™ Deep了解更多信息
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货号数量
C104481 kit
货号 C10448
价格(CNY)
5,189.00
飞享价
Ends: 31-Dec-2025
6,806.00
共减 1,617.00 (24%)
Each
添加至购物车
数量:
1 kit
价格(CNY)
5,189.00
飞享价
Ends: 31-Dec-2025
6,806.00
共减 1,617.00 (24%)
Each
添加至购物车
CellROX™ 一件混合装包含 CellROX™ Green 试剂、CellROX™ Orange 试剂和 CellROX™ Deep Red 试剂各一瓶 (50 µL)。这三种试剂是用于测量活细胞氧化应激的新型荧光探针。这些细胞渗透染料经活性氧 (ROS) 氧化后发出明亮的荧光。(关于每种试剂的详细信息,见下表。)

CellROX™ 试剂:

•经优化可用于检测活细胞中的氧化应激,且可以直接加入含血清的生长培养基中
•以稳定的即用型 DMSO 溶液形式提供,具有简单的方案,且与荧光显微镜的标准工作流程兼容
•与多种检测平台兼容,包括传统荧光显微镜、高内涵筛选 (HCS) 和流式细胞仪

氧化应激是活性氧 (ROS) 生成能力与细胞的清除能力失衡造成的。ROS 在多种疾病(包括炎症、动脉粥样硬化、衰老和年龄相关退行性疾病)的进展中发挥重要作用。

 货号吸收/发射(颜色)适用于活细胞?与甲醛固定兼容?耐洗涤剂?检测平台
CellROX™ Deep Red 试剂C10422644/665 nm (Deep Red)成像,HCS,HTS,流式细胞分析,Attune™
CellROX™ Orange 试剂C10443545/565 nm (Orange)成像,HCS,流式细胞仪,Tali™
CellROX™ Green 试剂C10444485/520 nm (Green)成像,HCS,HTS,流式,Tali™,FLoid™,Attune™


仅供科研使用。不得用于任何动物或人类的治疗或诊断。
仅供科研使用。不可用于诊断程序。
规格
颜色橙色、远红、绿色
产品规格冰冻
数量1 kit
Unit SizeEach

常见问题解答 (FAQ)

哪些染料可以用来检测细菌内的活性氧(ROS)?

许多对哺乳动物细胞使用的染料也可以用于细菌。例如,CellROX Deep Red试剂经证实可以用于检测B. subtilis的活性氧(见参考文献http://www-brs.ub.ruhr-uni-bochum.de/netahtml/HSS/Diss/RaatschenNadja/diss.pdf)。如果您对某一特定染料有兴趣但不知道它对您的细菌是否有效,最佳途径是通过文献检索的方式查看它是否已经过试验。如果没有,则可能需要您亲自测试。

What dyes can I use to detect reactive oxygen species (ROS) in my bacteria?

Many dyes that are used on mammalian cells have also been shown to be useful in bacterial cells. For example, CellROX Deep Red Reagent has been shown to work in B. subtilis (see Reference: http://www-brs.ub.ruhr-uni-bochum.de/netahtml/HSS/Diss/RaatschenNadja/diss.pdf). If you are interested in a particular dye, but are not sure if it will work on your bacteria, literature searches are the best way to check to see if it has been tested. If not, then it may be worth testing yourself.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (15)

引用和文献
Abstract
Mechanisms of programmed cell death signaling in hair cells and support cells post-electrode insertion trauma.
Authors:Eshraghi AA, Lang DM, Roell J, Van De Water TR, Garnham C, Rodrigues H, Guardiola M, Gupta C, Mittal J,
Journal:
PubMed ID:25761716
'Programmed cell death (PCD) initially starts in the support cells (SCs) after electrode insertion trauma (EIT), followed by PCD in hair cells (HCs). Activation of caspase-3 was observed only in SCs. Protecting both SCs and HCs with selective otoprotective drugs at an early stage post implantation may help to preserve ... More
Dynamics of sub-lethal effects of nano-CuO on the microalga Chlamydomonas reinhardtii during short-term exposure.
Authors:von Moos N, Maillard L, Slaveykova VI,
Journal:
PubMed ID:25731685
Though nano-CuO has been classified as toxic toward aquatic microorganisms and its use in various applications is expected to increase in near future, its ecotoxicity is currently poorly understood. The aim of this study was to investigate the mechanisms of nano-CuO toxicity based on the paradigm of oxidative stress, the ... More
Development of poly(ethylene glycol) hydrogels for salivary gland tissue engineering applications.
Authors:Shubin AD, Felong T, Graunke D, Ovitt CE, Benoit D,
Journal:
PubMed ID:25762214
Over 40,000 patients are diagnosed with head and neck cancers annually in the US with the vast majority receiving radiation therapy. Salivary glands are irreparably damaged by radiation therapy resulting in xerostomia, which severely affects patient quality of life. Cell-based therapies have shown some promise in mouse models of radiation-induced ... More
Oxidative stress triggers cytokinesis failure in hepatocytes upon isolation.
Authors:Tormos AM, Taléns-Visconti R, Bonora-Centelles A, Pérez S, Sastre J,
Journal:
PubMed ID:25744598
Primary hepatocytes are highly differentiated cells and proliferatively quiescent. However, the stress produced during liver digestion seems to activate cell cycle entry by proliferative/dedifferentiation programs that still remain unclear. The aim of this work was to assess whether the oxidative stress associated with hepatocyte isolation affects cell cycle and particularly ... More
DNA Damage: A Sensible Mediator of the Differentiation Decision in Hematopoietic Stem Cells and in Leukemia.
Authors:Weiss CN, Ito K,
Journal:
PubMed ID:25789504
In the adult, the source of functionally diverse, mature blood cells are hematopoietic stem cells, a rare population of quiescent cells that reside in the bone marrow niche. Like stem cells in other tissues, hematopoietic stem cells are defined by their ability to self-renew, in order to maintain the stem ... More