Using transcriptomics to improve butanol tolerance in Synechocystis sp. PCC 6803.
AuthorsAnfelt J, Hallström B, Nielsen JB, Uhlén M, Hudson EP,
Journal
PubMed ID24056459
Cyanobacteria are emerging as promising hosts for production of advanced biofuels such as n-butanol and alkanes. However, cyanobacteria suffer from the same product inhibition problems that plague other microbial biofuel hosts. High concentrations of butanol severely reduce the growth and even low amounts can negatively affect metabolic processes. An understanding ... More
MTI-101 (Cyclized HYD1) Binds a CD44 Containing Complex and Induces Necrotic Cell Death in Multiple Myeloma.
Our laboratory recently reported that treatment with the d-amino acid containing peptide HYD1 induces necrotic cell death in multiple myeloma cell lines. Because of the intriguing biological activity and promising in vivo activity of HYD1, we pursued strategies for increasing the therapeutic efficacy of the linear peptide. These efforts led ... More
Electrochemical Potential Gradient as a Quantitative in Vitro Test Platform for Cellular Oxidative Stress.
AuthorsBryant C, Atha D, Reipa V
JournalAntioxidants (Basel)
PubMed ID27409641
Oxidative stress in a biological system is often defined as a redox imbalance within cells or groups of cells within an organism. Reductive-oxidative (redox) imbalances in cellular systems have been implicated in several diseases, such as cancer. To better understand the redox environment within cellular systems, it is important to ... More
Production of Superoxide in Bacteria Is Stress- and Cell State-Dependent: A Gating-Optimized Flow Cytometry Method that Minimizes ROS Measurement Artifacts with Fluorescent Dyes.
AuthorsMcBee ME, Chionh YH, Sharaf ML, Ho P, Cai MW, Dedon PC
JournalFront Microbiol
PubMed ID28377755
The role of reactive oxygen species (ROS) in microbial metabolism and stress response has emerged as a major theme in microbiology and infectious disease. Reactive fluorescent dyes have the potential to advance the study of ROS in the complex intracellular environment, especially for high-content and high-throughput analyses. However, current dye-based ... More
Coral cell separation and isolation by fluorescence-activated cell sorting (FACS).
AuthorsRosental B, Kozhekbaeva Z, Fernhoff N, Tsai JM, Traylor-Knowles N
JournalBMC Cell Biol
PubMed ID28851289
Generalized methods for understanding the cell biology of non-model species are quite rare, yet very much needed. In order to address this issue, we have modified a technique traditionally used in the biomedical field for ecological and evolutionary research. Fluorescent activated cell sorting (FACS) is often used for sorting and ... More
Chondrocytes differentiated from human induced pluripotent stem cells: Response to ionizing radiation.
AuthorsStelcer E, Kulcenty K, Suchorska WM
JournalPLoS One
PubMed ID30352062
Data on the response of chondrocytes differentiated from hiPSCs (hiPSC-DCHs) to ionizing radiation (IR) are lacking. The aim of present study was to assess DNA damage response (DDR) mechanisms of IR-treated hiPSC-DCHs. ... More
Ibrutinib Resistance Is Reduced by an Inhibitor of Fatty Acid Oxidation in Primary CLL Lymphocytes.
AuthorsGalicia-Vázquez G, Aloyz R
JournalFront Oncol
PubMed ID30319974
Chronic Lymphocytic Leukemia (CLL) is an incurable disease, characterized by the accumulation of malignant B-lymphocytes in the blood stream (quiescent state) and homing tissues (where they can proliferate). In CLL, the targeting of B-cell receptor signaling through a Burton's tyrosine kinase inhibitor (ibrutinib) has rendered outstanding clinical results. However, complete ... More
Voluntary exercise reverses immune aging induced by oxidative stress in aging mice.
AuthorsLee JY, Paik IY, Kim JY
JournalExp Gerontol
PubMed ID30189231
Excessive oxidative stress leads to aging due to persistent damage to the cells, tissues, and the entire organism. Immunosenescence is also a devastating consequence of oxidative stress, but there is a lack of research on effective ways to overcome it. In this study, we used physiologic and immunological aging mouse ... More
Dosing depending on SIRT3 activity attenuates doxorubicin-induced cardiotoxicity via elevated tolerance against mitochondrial dysfunction and oxidative stress.
AuthorsYang N, Ma H, Jiang Z, Niu L, Zhang X, Liu Y, Wang Y, Cheng S, Deng Y, Qi H, Wang Z
JournalBiochem Biophys Res Commun
PubMed ID31303273
Doxorubicin (DOX) is a potent anti-neoplastic agent with cumulative cardiotoxicity. DOX-induced cardiotoxicity has been shown to depend on the different dosing times. However, the basis for determining the dosing time to minimize DOX-induced cardiotoxicity and the underlying mechanisms remain incompletely understood. Here we first showed that SIRT3, the major mitochondrial ... More
Autophagy-dependent cancer cells circumvent loss of the upstream regulator RB1CC1/FIP200 and loss of LC3 conjugation by similar mechanisms.
AuthorsTowers CG, Wodetzki D, Thorburn A
JournalAutophagy
PubMed ID32160093
Macroautophagy/autophagy degrades proteins and organelles to generate macromolecular building blocks. As such, some cancer cells are particularly dependent on autophagy. In a previous paper, we found that even highly autophagy-dependent cancer cells can adapt to circumvent autophagy inhibition. However, it remains unclear if autophagy-dependent cancer cells could survive the complete ... More
Smad2/3-pathway ligand trap luspatercept enhances erythroid differentiation in murine ß-thalassaemia by increasing GATA-1 availability.
AuthorsMartinez PA, Li R, Ramanathan HN, Bhasin M, Pearsall RS, Kumar R, Suragani RNVS
JournalJ Cell Mol Med
PubMed ID32351032
In ß-thalassaemia, anaemia results from ineffective erythropoiesis characterized by inhibition of late-stage erythroid differentiation. We earlier used luspatercept and RAP-536 protein traps for certain Smad2/3-pathway ligands to implicate Smad2/3-pathway overactivation in dysregulated erythroid differentiation associated with murine ß-thalassaemia and myelodysplasia. Importantly, luspatercept alleviates anaemia and has been shown to reduce ... More
Tumor necrosis factor receptor-2 signaling pathways promote survival of cancer stem-like CD133