Fluorescent probes for the detection of reactive oxygen species in human spermatozoa.
AuthorsEscada-Rebelo S, Mora FG, Sousa AP, Almeida-Santos T, Paiva A, Ramalho-Santos J
Journal
PubMed ID31939350
'Reactive oxygen species (ROS) production is a by-product of mitochondrial activity and is necessary for the acquisition of the capacitated state, a requirement for functional spermatozoa. However, an increase in oxidative stress, due to an abnormal production of ROS, has been shown to be related to loss of sperm function, ... More
MicroRNA-27 (miR-27) Targets Prohibitin and Impairs Adipocyte Differentiation and Mitochondrial Function in Human Adipose-derived Stem Cells.
AuthorsKang T, Lu W, Xu W, Anderson L, Bacanamwo M, Thompson W, Chen YE, Liu D,
Journal
PubMed ID24133204
Prohibitin (PHB) has been reported to play a crucial role in adipocyte differentiation and mitochondrial function. However, the regulative mechanism of PHB during adipogenesis remains unclear. In this study, we determined that the levels of both microRNA (miR)-27a and miR-27b were down-regulated following adipogenic induction of human adipose-derived stem cells, ... More
Hypoxia and IF1 Expression Promote ROS Decrease in Cancer Cells.
AuthorsSgarbi G, Gorini G, Liuzzi F, Solaini G, Baracca A
JournalCells
PubMed ID29933600
'The role of reactive oxygen species (ROS) in the metabolic reprogramming of cells adapted to hypoxia and the interplay between ROS and hypoxia in malignancy is under debate. Here, we examined how ROS levels are modulated by hypoxia in human cancer compared to untransformed cells. Short time exposure (20 min) ... More
Isolation of strawberry anthocyanin-rich fractions and their mechanisms of action against murine breast cancer cell lines.
AuthorsMazzoni L, Giampieri F, Alvarez Suarez JM, Gasparrini M, Mezzetti B, Forbes Hernandez TY, Battino MA
JournalFood Funct
PubMed ID31621765
'The aim of this study was the evaluation of the effects of strawberry anthocyanin extract treatment on two in vitro models of murine breast cancer cell lines, in an attempt to detect a specific pathway (AMP-activated protein kinase or AMPK) through which strawberries exert their anticancer activity. The anticancer activity ... More
Berberine Effects on NF?B, HIF1A and NFE2L2/AP-1 Pathways in HeLa Cells.
AuthorsBelanova A, Beseda D, Chmykhalo V, Stepanova A, Belousova M, Khrenkova V, Gavalas N, Zolotukhin P
JournalAnticancer Agents Med Chem
PubMed ID30526471
'Berberine has multitudinous anti-cancer stem cells effects making it a highly promising candidate substance for the next-generation cancer therapy. However, berberine modes of action predispose it to significant side-effects that probably limit its clinical testing and application.' ... More
The novel triterpenoid RTA 408 protects human retinal pigment epithelial cells against H2O2-induced cell injury via NF-E2-related factor 2 (Nrf2) activation.
AuthorsLiu X, Ward K, Xavier C, Jann J, Clark AF, Pang IH, Wu H
JournalRedox Biol
PubMed ID26773873
Oxidative stress-induced retinal pigment epithelial (RPE) cell damage is an important factor in the pathogenesis of age-related macular degeneration (AMD). Previous studies have shown that RTA 408, a synthetic triterpenoid compound, potently activates Nrf2. This study aimed to investigate the protective effects of RTA 408 in cultured RPE cells during ... More
Salidroside prevents hydroperoxide-induced oxidative stress and apoptosis in retinal pigment epithelium cells.
AuthorsYin Y, Liu D, Tian D
JournalExp Ther Med
PubMed ID30210588
Salidroside (SAL) is the major pharmacologically active constituent of
Single identical cell toxicity assay on coordinately ordered patterns.
AuthorsLi Q, Ma L, Su M
JournalAnal Chim Acta
PubMed ID31005151
Toxicity assay is crucial in identifying biological mechanisms, detecting disease and screening therapeutics. Single cell toxicity assay reveals heterogeneous responses of each cell without ensemble average, but still cannot indicate responses of the same individual cells to multiple treatments. This article reports an identical cell assay that can be used ... More
Efficiency of CellROX deep red
AuthorsCeleghini ECC, Alves MBR, de Arruda RP, de Rezende GM, Florez-Rodriguez SA, de Sá Filho MF
JournalAnim Biotechnol
PubMed ID31424334
Detection of reactive oxygen species (ROS) is of great interest in semen analysis since their excess is detrimental to sperm function and male fertility. Fluorescence microscopy has achieved attention for providing broad possibilities of sperm evaluations and also for presenting substantial accessibility. In this context, this study investigated the efficiency ... More
Anti-inflammatory and immune-modulating effects of rice callus suspension culture (RCSC) and bioactive fractions in an in vitro inflammatory bowel disease model.
AuthorsDriscoll K, Deshpande A, Chapp A, Li K, Datta R, Ramakrishna W
JournalPhytomedicine
PubMed ID30831485
Rice callus suspension culture (RCSC) has been shown to exhibit potent antiproliferative activity in multiple cancer cell lines. RCSC and its bioactive compounds can fill the need for drugs with no side effects. ... More
Anti-inflammatory Effects of Northern Highbush Blueberry Extract on an
AuthorsDriscoll K, Deshpande A, Datta R, Ramakrishna W
JournalNutr Cancer
PubMed ID31588794
Blueberry anthocyanins have the ability to efficiently reach the GI tract and exhibit a broad range of biochemical effects. In the context of inflammatory bowel disease (IBD), they remain a promising complement to current IBD treatments. Here, we investigated the anti-inflammatory and antioxidant capabilities of Highbush blueberries ... More