CapZ and actin capping dynamics increase in myocytes after a bout of exercise and abates in hours after stimulation ends.
AuthorsLin YH, Li J, Swanson ER, Russell B,
JournalJ Appl Physiol (1985)
PubMed ID23493359
'The time course of the response and recovery after acute activity seen in exercise is not well understood. The goal of this work is to address how proteins of the thin filament (actin and its capping protein CapZ) are changed by 1 h of mechanical stimulation and return to baseline ... More
Stress fibers stabilize the position of intranuclear DNA through mechanical connection with the nucleus in vascular smooth muscle cells.
AuthorsNagayama K, Yahiro Y, Matsumoto T,
JournalFEBS Lett
PubMed ID22094165
Actin stress fibers (SFs) running across the top surface of the nucleus in vascular smooth muscle cells were dissected using laser nano-dissection technique to release its pretension, and the dynamic behavior of SFs, nucleus, and intranuclear DNA were investigated. SFs shortened across the top surface of the nuclei after their ... More
Viscoelastic cell mechanics and actin remodelling are dependent on the rate of applied pressure.
AuthorsPravincumar P, Bader DL, Knight MM,
JournalPLoS One
PubMed ID22984454
Living cells are subjected to external and internal mechanical stresses. The effects of these stresses on the deformation and subsequent biological response of the cells remains unclear. This study tested the hypothesis that the rate at which pressure (or stress) is applied influence the viscoelastic properties of the cell associated ... More
Baculovirus-mediated gene transfer into mammalian cells.
AuthorsBoyce FM, Bucher NL,
JournalProc Natl Acad Sci U S A
PubMed ID8637876
This paper describes the use of the baculovirus Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) as a vector for gene delivery into mammalian cells. A modified AcMNPV virus was prepared that carried the Escherichia coli lacZ reporter gene under control of the Rous sarcoma virus promoter and mammalian RNA processing ... More