CellLight™ 肌动蛋白-GFP (BacMam 2.0)
CellLight™ 肌动蛋白-GFP (BacMam 2.0)
引用和文献 (18)
Invitrogen™

CellLight™ 肌动蛋白-GFP (BacMam 2.0)

CellLight™ 肌动蛋白-GFP (BacMam 2.0) 可采用绿色荧光蛋白 (GFP) 轻松标记活细胞中的肌动蛋白。您只需要将试剂加入到细胞中，过夜孵育，第二天早上即可直接进行成像观察。想要标记其他细胞结构？了解更多有关 CellLight™了解更多信息
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货号数量靶标
C105821 瓶肌动蛋白、细胞骨架
货号 C10582
价格（CNY)
8,557.00
Each
添加至购物车
数量:
1 瓶
靶标:
肌动蛋白、细胞骨架
价格（CNY)
8,557.00
Each
添加至购物车
CellLight™ 肌动蛋白-GFP (BacMam 2.0) 可采用绿色荧光蛋白 (GFP) 轻松标记活细胞中的肌动蛋白。您只需要将试剂加入到细胞中，过夜孵育，第二天早上即可直接进行成像观察。

想要标记其他细胞结构？了解更多有关 CellLight™ 荧光蛋白标记工具的信息

这种即用型构建体可利用 BacMam 2.0技术转染到细胞中并表达融合人肌动蛋白的 GFP，且几乎无任何细胞毒性效应。研究活细胞中的肌动蛋白微丝动态作用，以及结合其他荧光蛋白或有机染料进行多重分析。

表达 CellLight™ 构建体的细胞也可用甲醛固定，以便使用免疫细胞化学技术进行多重成像分析。

CellLight™ 技术具有如下特性：
•快速便捷：只需将 CellLight™ 试剂加入细胞，过夜孵育，随后即可成像—或冷冻储存，以便后续实验直接使用
• 高效：转导率高达 90%，适用于多种哺乳动物细胞系，包括原代细胞、干细胞和神经元
• 灵活：在多重分析实验或共定位研究中，可共转导多种 BacMam 试剂；通过简单调整剂量即可严格控制表达水平
• 低毒性：CellLight™ 试剂不会在哺乳动物细胞中复制，适用于生物安全等级 (BSL) 1 处理

BacMam 技术
CellLight™ 肌动蛋白-GFP (BacMam 2.0) 是人肌动蛋白和 emGFP 的融合构建体，可准确和特异性靶向细胞肌动蛋白-GFP。这种融合构建体包装在昆虫病毒杆状病毒中，不会在人细胞中复制，在大多数实验室中被指定为生物安全等级 (BSL) 1，可安全使用。BacMam 技术可高效、超低毒性转导/转染大多数哺乳动物细胞类型。这种瞬时转染检测可从过夜孵育后持续最多五天 — 足以完成大部分动态细胞分析。与所有转染/转导技术一样，BacMam 方法无法以同等效率转染/转导所有细胞，因此不太适合细胞群研究或自动成像/计数。CellLight™ 试剂非常适合细胞或亚细胞共定位实验，以及需要特殊分辨率的细胞功能研究。
仅供科研使用。不可用于诊断程序。
规格
颜色绿色
检测方法荧光
染料类型GFP (EmGFP)
发射可见光
激发波长范围488⁄510
适用于（设备）共聚焦显微镜、荧光显微镜
形式液体
产品线CellLight
数量1 瓶
运输条件湿冰
靶标肌动蛋白、细胞骨架
技术荧光强度
标签类型荧光蛋白
产品类型肌动蛋白Actin
亚细胞定位肌动蛋白、细胞骨架, Cytoskeleton
Unit SizeEach
内容与储存
在 2°C 至 6°C 下避光储存。切勿冷冻。

常见问题解答 (FAQ)

我采用CellLight 标记试剂处理神经元时，转导效率很低，该如何提高转染效率？

与许多其它细胞相比，神经元转导更为困难。提高转导效率的主要方法是采用更多数量的病毒颗粒标记细胞。对于原代神经元，在铺盘时转导要比已培养细胞转导效果更好。此外，蛋白的表达在神经元中发生较慢，表达高峰通常发生于2-3天后而非转导后16小时。

How can I increase the transduction efficiency with the BacMam 2.0 reagents such as the the CellLight and Premo products?

Try varying particle-to-cell ratio (PPC), incubation volume, temperature and, cell density (if adherent cells are transduced). For adherent cells, we recommend a confluence of about 70%. Following the PPC, adjusting the volume is the next best parameter to change to optimize protein expression. If that doesn't work, you can also use the BacMam Enhancer Kit (Cat. No. B10107).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is there any way to preserve the CellLights labeling beyond 5 days?

Cells transduced with the CellLights reagents can be stored frozen for several months after transduction, without loss of expression.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Are the CellLights products toxic to cells?

If the viral particles are used at the level we recommend, they are very well tolerated by cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

For how long will the CellLights products label my cells?

The BacMam 2.0 CellLights typically express for 5 days after transduction.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all CellLight™ 肌动蛋白-GFP (BacMam 2.0) FAQs

引用和文献 (18)

引用和文献
Abstract
CapZ and actin capping dynamics increase in myocytes after a bout of exercise and abates in hours after stimulation ends.
Authors:Lin YH, Li J, Swanson ER, Russell B,
Journal:J Appl Physiol (1985)
PubMed ID:23493359
'The time course of the response and recovery after acute activity seen in exercise is not well understood. The goal of this work is to address how proteins of the thin filament (actin and its capping protein CapZ) are changed by 1 h of mechanical stimulation and return to baseline ... More
Stress fibers stabilize the position of intranuclear DNA through mechanical connection with the nucleus in vascular smooth muscle cells.
Authors:Nagayama K, Yahiro Y, Matsumoto T,
Journal:FEBS Lett
PubMed ID:22094165
Actin stress fibers (SFs) running across the top surface of the nucleus in vascular smooth muscle cells were dissected using laser nano-dissection technique to release its pretension, and the dynamic behavior of SFs, nucleus, and intranuclear DNA were investigated. SFs shortened across the top surface of the nuclei after their ... More
Human disease locus discovery and mapping to molecular pathways through phylogenetic profiling.
Authors:Tabach Y, Golan T, Hernández-Hernández A, Messer AR, Fukuda T, Kouznetsova A, Liu JG, Lilienthal I, Levy C, Ruvkun G,
Journal:
PubMed ID:24084807
Genes with common profiles of the presence and absence in disparate genomes tend to function in the same pathway. By mapping all human genes into about 1000 clusters of genes with similar patterns of conservation across eukaryotic phylogeny, we determined that sets of genes associated with particular diseases have similar ... More
Phosphatidylinositol 4,5-bisphosphate regulates CapZß1 and actin dynamics in response to mechanical strain.
Authors:Li J, Russell B,
Journal:
PubMed ID:24043251
Mechanical stress causes filament remodeling leading to myocyte hypertrophy and heart failure. The actin capping protein Z (CapZ) tightly binds to the barbed end of actin filaments, thus regulating actin assembly. The hypothesis is that the binding between CapZ and the actin filament is modulated through phosphatidylinositol 4,5-bisphosphate (PIP2) and ... More
Evaluation of nanoparticle uptake in co-culture cancer models.
Authors:Costa EC, Gaspar VM, Marques JG, Coutinho P, Correia IJ,
Journal:
PubMed ID:23922909
Co-culture models are currently bridging the gap between classical cultures and in vivo animal models. Exploring this novel approach unlocks the possibility to mimic the tumor microenvironment in vitro, through the establishment of cancer-stroma synergistic interactions. Notably, these organotypic models offer a perfect platform for the development and pre-clinical evaluation ... More
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