'Monocarboxylate transporter 8 (MCT8) is a thyroid hormone (TH)-specific transporter. Mutations in the MCT8 gene are associated with Allan-Herndon-Dudley Syndrome (AHDS), consisting of severe psychomotor retardation and disturbed TH parameters. To study the functional consequences of different MCT8 mutations in detail, we combined functional analysis in different cell types with ... More
Single-cell redox imaging demonstrates a distinctive response of dopaminergic neurons to oxidative insults.
AuthorsHorowitz MP, Milanese C, Di Maio R, Hu X, Montero LM, Sanders LH, Tapias V, Sepe S, van Cappellen WA, Burton EA, Greenamyre JT, Mastroberardino PG,
JournalAntioxid Redox Signal
PubMed ID21395478
'The study of the intracellular oxido-reductive (redox) state is of extreme relevance to the dopamine (DA) neurons of the substantia nigra pars compacta. These cells possess a distinct physiology intrinsically associated with elevated reactive oxygen species production, and they selectively degenerate in Parkinson''s disease under oxidative stress conditions. To test ... More
Intracellular trafficking mechanism, from intracellular uptake to extracellular efflux, for phospholipid/cholesterol liposomes.
AuthorsUn K, Sakai-Kato K, Oshima Y, Kawanishi T, Okuda H,
JournalBiomaterials
PubMed ID22858002
'Liposomes are widely used as drug delivery vehicles to transfer chemotherapeutic agents, proteins, and nucleic acids into target cells. To improve therapeutic effects and reduce unexpected toxic side-effects, it is necessary to understand the mechanism of liposomal uptake into cells, and the intracellular fate of internalized liposomes. The intracellular fate ... More
BPIFB3 regulates autophagy and coxsackievirus B replication through a noncanonical pathway independent of the core initiation machinery.
AuthorsDelorme-Axford E, Morosky S, Bomberger J, Stolz DB, Jackson WT, Coyne CB,
Journal
PubMed ID25491355
'Enteroviruses require autophagy to facilitate the formation of autophagosome (AP)-like double-membrane vesicles that provide the scaffolding for RNA replication. Here, we identify bactericidal/permeability-increasing protein (BPI) fold-containing family B, member 3 (BPIFB3) as a gene whose silencing greatly enhances coxsackievirus B (CVB) replication and induces dramatic alterations in the morphology of ... More
The amyloid precursor protein copper binding domain histidine residues 149 and 151 mediate APP stability and metabolism.
One of the key pathological hallmarks of Alzheimer disease (AD) is the accumulation of the APP-derived amyloid ß peptide (Aß) in the brain. Altered copper homeostasis has also been reported in AD patients and is thought to increase oxidative stress and to contribute to toxic Aß accumulation and regulate APP ... More
A novel pathogenic role of the ER chaperone GRP78/BiP in rheumatoid arthritis.
AuthorsYoo SA, You S, Yoon HJ, Kim DH, Kim HS, Lee K, Ahn JH, Hwang D, Lee AS, Kim KJ, Park YJ, Cho CS, Kim WU,
JournalJ Exp Med
PubMed ID22430489
An accumulation of misfolded proteins can trigger a cellular survival response in the endoplasmic reticulum (ER). In this study, we found that ER stress-associated gene signatures were highly expressed in rheumatoid arthritis (RA) synoviums and synovial cells. Proinflammatory cytokines, such as TNF and IL-1ß, increased the expression of GRP78/BiP, a ... More
Baculovirus-mediated gene transfer into mammalian cells.
AuthorsBoyce FM, Bucher NL,
JournalProc Natl Acad Sci U S A
PubMed ID8637876
This paper describes the use of the baculovirus Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) as a vector for gene delivery into mammalian cells. A modified AcMNPV virus was prepared that carried the Escherichia coli lacZ reporter gene under control of the Rous sarcoma virus promoter and mammalian RNA processing ... More
BacMam technology and its application to drug discovery.
AuthorsAmes RS, Kost TA, Condreay JP,
JournalExpert Opin Drug Discov
PubMed ID23488908
The recombinant baculovirus/insect cell system was firmly established as a leading method for recombinant protein production when a new potential use for these viruses was revealed in 1995. It was reported that engineered recombinant baculoviruses could deliver functional expression cassettes to mammalian cell types; a system which has come to ... More
TEX264 Is an Endoplasmic Reticulum-Resident ATG8-Interacting Protein Critical for ER Remodeling during Nutrient Stress.
AuthorsAn H, Ordureau A, Paulo JA, Shoemaker CJ, Denic V, Harper JW
JournalMol Cell
PubMed ID31006537
'Cells respond to nutrient stress by trafficking cytosolic contents to lysosomes for degradation via macroautophagy. The endoplasmic reticulum (ER) serves as an initiation site for autophagosomes and is also remodeled in response to nutrient stress through ER-phagy, a form of selective autophagy. Quantitative proteome analysis during nutrient stress identified an ... More
Spatial control of nucleoporin condensation by fragile X-related proteins.
Authors
JournalEMBO J
PubMed ID32706158
Biallelic Variants in UBA5 Reveal that Disruption of the UFM1 Cascade Can Result in Early-Onset Encephalopathy.
Authors
JournalAm J Hum Genet
PubMed ID27545681
Immuno-potentiating pathway of HBsAg-HBIG immunogenic complex visualized.
AuthorsLiu H, Geng S, Wang B, Wu B, Xie X, Wang S, Zhong Y, Wang X, Qu D, Wen Y, Wang B,
JournalHum Vaccin Immunother
PubMed ID26618396
'Chronic viral hepatitis B (CHB) is a major global health problem. A therapeutic vaccine for CHB comprised of yeast-derived recombinant HBsAg-anti-HBs immunogenic complexes (YIC) has been devloped by us. A series of clinical trials has shown its therapeutic efficacy in decreasing HBV viral load and converting serum HBeAg-positive to anti-HBe-positive ... More
Odorant Receptors Containing Conserved Amino Acid Sequences in Transmembrane Domain 7 Display Distinct Expression Patterns in Mammalian Tissues.
Authors
JournalMol Cells
PubMed ID29179263
Long-chain ceramides are cell non-autonomous signals linking lipotoxicity to endoplasmic reticulum stress in skeletal muscle.
Authors
JournalNat Commun
PubMed ID35365625
CAND1 regulates lunapark for the proper tubular network of the endoplasmic reticulum.
Authors
JournalSci Rep
PubMed ID31511573
A conserved sequence in the small intracellular loop of tetraspanins forms an M-shaped inter-helix turn.
Authors
JournalSci Rep
PubMed ID35296690
Classes of non-conventional tetraspanins defined by alternative splicing.
Authors
JournalSci Rep
PubMed ID31575878
Staurosporines disrupt phosphatidylserine trafficking and mislocalize Ras proteins.
Authors
JournalJ Biol Chem
PubMed ID23124205
Transit and integration of extracellular mitochondria in human heart cells.
Authors
JournalSci Rep
PubMed ID29234096
A small-molecule inhibitor of the ubiquitin activating enzyme for cancer treatment.
AuthorsHyer ML, Milhollen MA, Ciavarri J, Fleming P, Traore T, Sappal D, Huck J, Shi J, Gavin J, Brownell J, Yang Y, Stringer B, Griffin R, Bruzzese F, Soucy T, Duffy J, Rabino C, Riceberg J, Hoar K, Lublinsky A, Menon S, Sintchak M, Bump N, Pulukuri SM, Langston S, Tirrell S, Kuranda M, Veiby P, Newcomb J, Li P, Wu JT, Powe J, Dick LR, Greenspan P, Galvin K, Manfredi M, Claiborne C, Amidon BS, Bence NF
JournalNat Med
PubMed ID29334375
The ubiquitin-proteasome system (UPS) comprises a network of enzymes that is responsible for maintaining cellular protein homeostasis. The therapeutic potential of this pathway has been validated by the clinical successes of a number of UPS modulators, including proteasome inhibitors and immunomodulatory imide drugs (IMiDs). Here we identified TAK-243 (formerly known ... More
Endoplasmic reticulum mediates mitochondrial transfer within the osteocyte dendritic network.
AuthorsGao J, Qin A, Liu D, Ruan R, Wang Q, Yuan J, Cheng TS, Filipovska A, Papadimitriou JM, Dai K, Jiang Q, Gao X, Feng JQ, Takayanagi H, Zhang C, Zheng MH
JournalSci Adv
PubMed ID31799389
Mitochondrial transfer plays a crucial role in the regulation of tissue homeostasis and resistance to cancer chemotherapy. Osteocytes have interconnecting dendritic networks and are a model to investigate its mechanism. We have demonstrated, in primary murine osteocytes with photoactivatable mitochondria (PhAM) ... More
Mitochondrial Neuroglobin Is Necessary for Protection Induced by Conditioned Medium from Human Adipose-Derived Mesenchymal Stem Cells in Astrocytic Cells Subjected to Scratch and Metabolic Injury.
AuthorsBaez-Jurado E, Guio-Vega G, Hidalgo-Lanussa O, González J, Echeverria V, Ashraf GM, Sahebkar A, Barreto GE
JournalMol Neurobiol
PubMed ID30536184
Astrocytes are specialized cells capable of regulating inflammatory responses in neurodegenerative diseases or traumatic brain injury. In addition to playing an important role in neuroinflammation, these cells regulate essential functions for the preservation of brain tissue. Therefore, the search for therapeutic alternatives to preserve these cells and maintain their functions ... More