Substrate stiffness regulates cadherin-dependent collective migration through myosin-II contractility.
AuthorsNg MR, Besser A, Danuser G, Brugge JS,
JournalJ Cell Biol
PubMed ID23091067
'The mechanical microenvironment is known to influence single-cell migration; however, the extent to which mechanical cues affect collective migration of adherent cells is not well understood. We measured the effects of varying substrate compliance on individual cell migratory properties in an epithelial wound-healing assay. Increasing substrate stiffness increased collective cell ... More
Dynamic colocalization microscopy to characterize intracellular trafficking of nanomedicines.
AuthorsVercauteren D, Deschout H, Remaut K, Engbersen JF, Jones AT, Demeester J, De Smedt SC, Braeckmans K,
JournalACS Nano
PubMed ID21923168
'To gain a better understanding of intracellular processing of nanomedicines, we employed quantitative live-cell fluorescence colocalization microscopy to study endosomal trafficking of polyplexes in retinal pigment epithelium cells. A new, dynamic colocalization algorithm was developed, based on particle tracking and trajectory correlation, allowing for spatiotemporal characterization of internalized polyplexes in ... More
Intracellular trafficking mechanism, from intracellular uptake to extracellular efflux, for phospholipid/cholesterol liposomes.
AuthorsUn K, Sakai-Kato K, Oshima Y, Kawanishi T, Okuda H,
JournalBiomaterials
PubMed ID22858002
'Liposomes are widely used as drug delivery vehicles to transfer chemotherapeutic agents, proteins, and nucleic acids into target cells. To improve therapeutic effects and reduce unexpected toxic side-effects, it is necessary to understand the mechanism of liposomal uptake into cells, and the intracellular fate of internalized liposomes. The intracellular fate ... More
Glycophthalocyanines as photosensitizers for triggering mitotic catastrophe and apoptosis in cancer cells.
AuthorsSoares AR, Neves MG, Tomé AC, Iglesias-de la Cruz MC, Zamarrón A, Carrasco E, González S, Cavaleiro JA, Torres T, Guldi DM, Juarranz A,
JournalChem Res Toxicol
PubMed ID22394248
'Photodynamic therapy (PDT) is a treatment modality for different forms of cancer based on the combination of light, molecular oxygen, and a photosensitizer (PS) compound. When activated by light, the PS generates reactive oxygen species leading to tumor destruction. Phthalocyanines are compounds that have already shown to be efficient PSs ... More
A Review of Reagents for Fluorescence Microscopy of Cellular Compartments and Structures, Part III: Reagents for Actin, Tubulin, Cellular Membranes, and Whole Cell and Cytoplasm.
AuthorsKilgore JA, Dolman NJ, Davidson MW,
Journal
PubMed ID24510770
'Non-antibody commercial fluorescent reagents for imaging of cytoskeletal structures have been limited primarily to tubulin and actin, with the main factor in choice based mainly on whether cells are live or fixed and permeabilized. A wider range of options exist for cell membrane dyes, and the choice of reagent primarily ... More
Utilization of fluorescent probes for the quantification and identification of subcellular proteomes and biological processes regulated by lipid peroxidation products.
AuthorsCummins TD, Higdon AN, Kramer PA, Chacko BK, Riggs DW, Salabei JK, Dell'italia LJ, Zhang J, Darley-Usmar VM, Hill BG,
JournalFree Radic Biol Med
PubMed ID22954622
Oxidative modifications to cellular proteins are critical in mediating redox-sensitive processes such as autophagy, the antioxidant response, and apoptosis. The proteins that become modified by reactive species are often compartmentalized to specific organelles or regions of the cell. Here, we detail protocols for identifying the subcellular protein targets of lipid ... More
Quadriwave lateral shearing interferometry for quantitative phase microscopy of living cells.
Phase imaging with a high-resolution wavefront sensor is considered. This is based on a quadriwave lateral shearing interferometer mounted on a non-modified transmission white-light microscope. The measurement technology is explained both in the scope of wave optics and geometrical optics in order to discuss its implementation on a conventional microscope. ... More
LRRTM3 interacts with APP and BACE1 and has variants associating with late-onset Alzheimer's disease (LOAD).
AuthorsLincoln S, Allen M, Cox CL, Walker LP, Malphrus K, Qiu Y, Nguyen T, Rowley C, Kouri N, Crook J, Pankratz VS, Younkin S, Younkin L, Carrasquillo M, Zou F, Abdul-Hay SO, Springer W, Sando SB, Aasly JO, Barcikowska M, Wszolek ZK, Lewis JM, Dickson D, Graff-Radford NR, Petersen RC, Eckman E, Younkin SG, Ertekin-Taner N,
Journal
PubMed ID23750206
Leucine rich repeat transmembrane protein 3 (LRRTM3) is member of a synaptic protein family. LRRTM3 is a nested gene within a-T catenin (CTNNA3) and resides at the linkage peak for late-onset Alzheimer's disease (LOAD) risk and plasma amyloid ß (Aß) levels. In-vitro knock-down of LRRTM3 was previously shown to decrease ... More
Replication-competent influenza A virus that encodes a split-green fluorescent protein-tagged PB2 polymerase subunit allows live-cell imaging of the virus life cycle.
AuthorsAvilov SV, Moisy D, Munier S, Schraidt O, Naffakh N, Cusack S,
JournalJ Virol
PubMed ID22114331
Studies on the intracellular trafficking of influenza virus ribonucleoproteins are currently limited by the lack of a method enabling their visualization during infection in single cells. This is largely due to the difficulty of encoding fluorescent fusion proteins within the viral genome. To circumvent this limitation, we used the split-green ... More
G-protein signaling modulator-3 regulates heterotrimeric G-protein dynamics through dual association with Gß and Gai protein subunits.
Regulation of the assembly and function of G-protein heterotrimers (Ga·GDP/Gß?) is a complex process involving the participation of many accessory proteins. One of these regulators, GPSM3, is a member of a family of proteins containing one or more copies of a small regulatory motif known as the GoLoco (or GPR) ... More
Polyplexes traffic through caveolae to the Golgi and endoplasmic reticulum en route to the nucleus.
AuthorsReilly MJ, Larsen JD, Sullivan MO,
JournalMol Pharm
PubMed ID22420286
The cellular machinery involved in the internalization of nonviral gene carriers and their subsequent trafficking to the nucleus directly impacts their therapeutic efficiency. Hence, identifying key endocytic pathways and organelles that contribute to the successful transfer of polyplexes to the nucleus generates new opportunities for improving carrier design. Previously, we ... More
Fluorescence lifetime imaging unravels C. trachomatis metabolism and its crosstalk with the host cell.
AuthorsSzaszák M, Steven P, Shima K, Orzekowsky-Schröder R, Hüttmann G, König IR, Solbach W, Rupp J,
JournalPLoS Pathog
PubMed ID21779161
Chlamydia trachomatis is an obligate intracellular bacterium that alternates between two metabolically different developmental forms. We performed fluorescence lifetime imaging (FLIM) of the metabolic coenzymes, reduced nicotinamide adenine dinucleotides [NAD(P)H], by two-photon microscopy for separate analysis of host and pathogen metabolism during intracellular chlamydial infections. NAD(P)H autofluorescence was detected inside ... More
Baculovirus-mediated gene transfer into mammalian cells.
AuthorsBoyce FM, Bucher NL,
JournalProc Natl Acad Sci U S A
PubMed ID8637876
This paper describes the use of the baculovirus Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) as a vector for gene delivery into mammalian cells. A modified AcMNPV virus was prepared that carried the Escherichia coli lacZ reporter gene under control of the Rous sarcoma virus promoter and mammalian RNA processing ... More
BacMam technology and its application to drug discovery.
AuthorsAmes RS, Kost TA, Condreay JP,
JournalExpert Opin Drug Discov
PubMed ID23488908
The recombinant baculovirus/insect cell system was firmly established as a leading method for recombinant protein production when a new potential use for these viruses was revealed in 1995. It was reported that engineered recombinant baculoviruses could deliver functional expression cassettes to mammalian cell types; a system which has come to ... More
Identification and Characterization of a Novel Gene, dapr, Involved in Skeletal Muscle Differentiation and Protein Kinase B Signaling.
AuthorsVirtanen C, Paris J, Takahashi M,
JournalJ Biol Chem
PubMed ID19028694
The phosphatidylinositol 3-kinase (PI3K) signaling pathway has been associated with a variety of cellular functions ranging from cell cycle regulation to tissue development. Although years of research have extensively characterized this signaling pathway, little is known as to how specific cellular events are coordinated by its activation. Here we demonstrate ... More
Respiratory syncytial virus co-opts host mitochondrial function to favour infectious virus production.
Authors
JournalElife
PubMed ID31246170
Stress-Induced Cellular Clearance Is Mediated by the SNARE Protein ykt6 and Disrupted by α-Synuclein.
Authors
JournalNeuron
PubMed ID31648898
FAM20A mutations can cause enamel-renal syndrome (ERS).
Authors
JournalPLoS Genet
PubMed ID23468644
AGS3-dependent trans-Golgi network membrane trafficking is essential for compaction in mouse embryos.
Authors
JournalJ Cell Sci
PubMed ID33148610
Pooled Screens Identify GPR108 and TM9SF2 as Host Cell Factors Critical for AAV Transduction.
Authors
JournalMol Ther Methods Clin Dev
PubMed ID32280726
Nanoparticles restore lysosomal acidification defects: Implications for Parkinson and other lysosomal-related diseases.
Authors
JournalAutophagy
PubMed ID26761717
Acetyl-CoA flux from the cytosol to the ER regulates engagement and quality of the secretory pathway.
Authors
JournalSci Rep
PubMed ID33479349
Systematic analysis of ribophagy in human cells reveals bystander flux during selective autophagy.
Authors
JournalNat Cell Biol
PubMed ID29230017
Transit and integration of extracellular mitochondria in human heart cells.