CellLight™ 溶酶体-RFP (BacMam 2.0)
CellLight™ 溶酶体-RFP (BacMam 2.0)
引用和文献 (22)
Invitrogen™

CellLight™ 溶酶体-RFP (BacMam 2.0)

CellLight™ 溶酶体-RFP (BacMam 2.0) 可采用红色荧光蛋白 (RFP) 轻松标记活细胞中的溶酶体。您只需要将试剂加入到细胞中，过夜孵育，第二天早上即可直接进行成像观察。想要标记其他细胞结构？了解更多有关 CellLight™了解更多信息
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货号数量颜色
C105971 瓶红橙色、橙色
货号 C10597
价格（CNY)
8,533.00
Each
添加至购物车
数量:
1 瓶
颜色:
红橙色、橙色
价格（CNY)
8,533.00
Each
添加至购物车
CellLight™ 溶酶体-RFP (BacMam 2.0) 可采用红色荧光蛋白 (RFP) 轻松标记活细胞中的溶酶体。您只需要将试剂加入到细胞中，过夜孵育，第二天早上即可直接进行成像观察。

想要标记其他细胞结构？了解更多有关 CellLight™ 荧光蛋白标记工具的信息

这种即用型构建体可使用 BacMam 2.0 技术转染到细胞中，并在此表达融合到 lamp1（溶酶体相关膜蛋白 1）上的 RFP。您可以不受细胞器 pH 值影响，观察活细胞内的溶酶体-RFP 行为，并可采用多种追踪和示踪染料标记，以便对动态细胞过程进行成像。

表达 CellLight™ 构建体的细胞也可采用甲醛固定，以便利用免疫细胞化学技术进行多通路成像。

CellLight™ 技术特性：
•快速便捷：只需将 CellLight™ 试剂加入细胞，过夜孵育，随后即可成像—或冷冻储存，以便后续实验直接使用
• 高效：转导率高达 90%，适用于多种哺乳动物细胞系，包括原代细胞、干细胞和神经元
• 灵活：在多重分析实验或共定位研究中，可共转导多种 BacMam 试剂；通过简单调整剂量即可严格控制表达水平
• 低毒性：CellLight™ 试剂不会在哺乳动物细胞中复制，适于生物安全等级 (BSL) 1 级处理

BacMam 技术
CellLight™ 溶酶体-RFP (BacMam 2.0) 是 lamp1（溶酶体相关膜蛋白 1）和 TagRFP 的融合构建体，可准确、特异地靶向细胞溶酶体-RFP。这种融合构建体包装在昆虫病毒杆状病毒中，不会在人类细胞中复制，生物安全等级 (BSL) 1 级，被指定为可以在大多数实验室中安全使用。BacMam 技术可高效、超低毒性转导/转染大多数哺乳动物细胞类型。这种瞬时转染检测可从过夜孵育后持续最多五天 — 足以完成大部分动态细胞分析。与所有转染/转导技术一样，BacMam 方法无法以同等效率转染/转导所有细胞，因此不太适合细胞群研究或自动成像/计数。CellLight™ 试剂非常适合细胞或亚细胞共定位实验，以及需要特殊分辨率的细胞功能研究。
仅供科研使用。不可用于诊断程序。
规格
颜色红橙色、橙色
检测方法荧光
染料类型RFP (TagRFP)
发射可见光
激发波长范围555⁄584
适用于（设备）共聚焦显微镜、荧光显微镜
形式液体
产品线CellLight
数量1 瓶
运输条件湿冰
技术荧光强度
标签类型荧光蛋白
产品类型溶酶体探针
亚细胞定位溶酶体Lysosomes
Unit SizeEach
内容与储存
在 2°C 至 6°C 下避光储存。切勿冷冻。

常见问题解答 (FAQ)

我采用CellLight 标记试剂处理神经元时，转导效率很低，该如何提高转染效率？

与许多其它细胞相比，神经元转导更为困难。提高转导效率的主要方法是采用更多数量的病毒颗粒标记细胞。对于原代神经元，在铺盘时转导要比已培养细胞转导效果更好。此外，蛋白的表达在神经元中发生较慢，表达高峰通常发生于2-3天后而非转导后16小时。

How can I increase the transduction efficiency with the BacMam 2.0 reagents such as the the CellLight and Premo products?

Try varying particle-to-cell ratio (PPC), incubation volume, temperature and, cell density (if adherent cells are transduced). For adherent cells, we recommend a confluence of about 70%. Following the PPC, adjusting the volume is the next best parameter to change to optimize protein expression. If that doesn't work, you can also use the BacMam Enhancer Kit (Cat. No. B10107).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is there any way to preserve the CellLights labeling beyond 5 days?

Cells transduced with the CellLights reagents can be stored frozen for several months after transduction, without loss of expression.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Are the CellLights products toxic to cells?

If the viral particles are used at the level we recommend, they are very well tolerated by cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

For how long will the CellLights products label my cells?

The BacMam 2.0 CellLights typically express for 5 days after transduction.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all CellLight™ 溶酶体-RFP (BacMam 2.0) FAQs

引用和文献 (22)

引用和文献
Abstract
Cell entry and trafficking of human adenovirus bound to blood factor X is determined by the fiber serotype and not hexon:heparan sulfate interaction.
Authors:Corjon S, Gonzalez G, Henning P, Grichine A, Lindholm L, Boulanger P, Fender P, Hong SS,
Journal:PLoS One
PubMed ID:21637339
'Human adenovirus serotype 5 (HAdV5)-based vectors administered intravenously accumulate in the liver as the result of their direct binding to blood coagulation factor X (FX) and subsequent interaction of the FX-HAdV5 complex with heparan sulfate proteoglycan (HSPG) at the surface of liver cells. Intriguingly, the serotype 35 fiber-pseudotyped vector HAdV5F35 ... More
Particles on the move: intracellular trafficking and asymmetric mitotic partitioning of nanoporous polymer particles.
Authors:Yan Y, Lai ZW, Goode RJ, Cui J, Bacic T, Kamphuis MM, Nice EC, Caruso F,
Journal:
PubMed ID:23713907
'Nanoporous polymer particles (NPPs) prepared by mesoporous silica templating show promise as a new class of versatile drug/gene delivery vehicles owning to their high payload capacity, functionality, and responsiveness. Understanding the cellular dynamics of such particles, including uptake, intracellular trafficking, and distribution, is an important requirement for their development as ... More
GX15-070 (obatoclax) induces apoptosis and inhibits cathepsin D- and L-mediated autophagosomal lysis in antiestrogen-resistant breast cancer cells.
Authors:Schwartz-Roberts JL, Shajahan AN, Cook KL, Wärri A, Abu-Asab M, Clarke R,
Journal:Mol Cancer Ther
PubMed ID:23395885
'In estrogen receptor-positive (ER+) breast cancer cells, BCL2 overexpression contributes to antiestrogen resistance. Direct targeting of the antiapoptotic BCL2 members with GX15-070 (obatoclax), a BH3-mimetic currently in clinical development, is an attractive strategy to overcome antiestrogen resistance in some breast cancers. Recently, GX15-070 has been shown to induce both apoptosis ... More
The possible
Authors:Benjaminsen RV, Mattebjerg MA, Henriksen JR, Moghimi SM, Andresen TL,
Journal:Mol Ther
PubMed ID:23032976
Polycations such as polyethylenimine (PEI) are used in many novel nonviral vector designs and there are continuous efforts to increase our mechanistic understanding of their interactions with cells. Even so, the mechanism of polyplex escape from the endosomal/lysosomal pathway after internalization is still elusive. The  ... More
Inhibitors of intravesicular acidification protect against Shiga toxin in a pH-independent manner.
Authors:Dyve Lingelem AB, Bergan J, Sandvig K,
Journal:Traffic
PubMed ID:22132807
Shiga toxin inhibits protein synthesis after being transported from the cell surface to endosomes and retrogradely through the Golgi apparatus to the endoplasmic reticulum (ER) and into the cytosol. In this study, we have abolished proton gradients across internal membranes in different ways and investigated the effect on the various ... More
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