Standardized mitochondrial analysis gives new insights into mitochondrial dynamics and OPA1 function.
AuthorsChevrollier A, Cassereau J, Ferré M, Alban J, Desquiret-Dumas V, Gueguen N, Amati-Bonneau P, Procaccio V, Bonneau D, Reynier P,
JournalInt J Biochem Cell Biol
PubMed ID22433900
'Mitochondria form dynamic tubular networks through processes of fission and fusion. Defect in mitochondrial dynamics lead to various pathologies, including several common and some rare neurodegenerative disorders. OPA1 and MFN2 are two key players in mitochondrial fusion associated with Autosomal Dominant Optic Atrophy and Charcot Marie Tooth neuropathy type 2A ... More
High-resolution mapping reveals topologically distinct cellular pools of phosphatidylserine.
AuthorsFairn GD, Schieber NL, Ariotti N, Murphy S, Kuerschner L, Webb RI, Grinstein S, Parton RG,
JournalJ Cell Biol
PubMed ID21788369
'Phosphatidylserine (PS) plays a central role in cell signaling and in the biosynthesis of other lipids. To date, however, the subcellular distribution and transmembrane topology of this crucial phospholipid remain ill-defined. We transfected cells with a GFP-tagged C2 domain of lactadherin to detect by light and electron microscopy PS exposed ... More
Ketamine induces toxicity in human neurons differentiated from embryonic stem cells via mitochondrial apoptosis pathway.
AuthorsBosnjak ZJ, Yan Y, Canfield S, Muravyeva MY, Kikuchi C, Wells CW, Corbett JA, Bai X,
JournalCurr Drug Saf
PubMed ID22873495
'Ketamine is widely used for anesthesia in pediatric patients. Growing evidence indicates that ketamine causes neurotoxicity in a variety of developing animal models. Our understanding of anesthesia neurotoxicity in humans is currently limited by difficulties in obtaining neurons and performing developmental toxicity studies in fetal and pediatric populations. It may ... More
Faecal excretion of ciprofloxacin after a single oral dose and its effect on faecal bacteria in healthy volunteers.
AuthorsPecquet S, Ravoire S, Andremont A,
JournalJ Antimicrob Chemother
PubMed ID2211433
'High concentrations of ciprofloxacin have been shown to persist in the faeces of volunteers for several days after a week of oral treatment with this drug, which was also found to have a prolonged effect on aerobic Gram-negative intestinal bacteria. To determine whether a shorter course of ciprofloxacin would have ... More
Energy metabolism in human pluripotent stem cells and their differentiated counterparts.
AuthorsVarum S, Rodrigues AS, Moura MB, Momcilovic O, Easley CA, Ramalho-Santos J, Van Houten B, Schatten G,
JournalPLoS One
PubMed ID21698063
'BACKGROUND: Human pluripotent stem cells have the ability to generate all cell types present in the adult organism, therefore harboring great potential for the in vitro study of differentiation and for the development of cell-based therapies. Nonetheless their use may prove challenging as incomplete differentiation of these cells might lead ... More
Structure of parkin reveals mechanisms for ubiquitin ligase activation.
AuthorsTrempe JF, Sauvé V, Grenier K, Seirafi M, Tang MY, Ménade M, Al-Abdul-Wahid S, Krett J, Wong K, Kozlov G, Nagar B, Fon EA, Gehring K,
Journal
PubMed ID23661642
'Mutations in the PARK2 (parkin) gene are responsible for an autosomal recessive form of Parkinson''s disease. The parkin protein is a RING-in-between-RING E3 ubiquitin ligase that exhibits low basal activity. We describe the crystal structure of full-length rat parkin. The structure shows parkin in an autoinhibited state and provides insight ... More
Inhibition of AMP-activated protein kinase a (AMPKa) by doxorubicin accentuates genotoxic stress and cell death in mouse embryonic fibroblasts and cardiomyocytes: role of p53 and SIRT1.
AuthorsWang S, Song P, Zou MH,
JournalJ Biol Chem
PubMed ID22267730
'Doxorubicin, an anthracycline antibiotic, is widely used in cancer treatment. Doxorubicin produces genotoxic stress and p53 activation in both carcinoma and non-carcinoma cells. Although its side effects in non-carcinoma cells, especially in heart tissue, are well known, the molecular targets of doxorubicin are poorly characterized. Here, we report that doxorubicin ... More
Life cell quantification of mitochondrial membrane potential at the single organelle level.
AuthorsDistelmaier F, Koopman WJ, Testa ER, de Jong AS, Swarts HG, Mayatepek E, Smeitink JA, Willems PH,
JournalCytometry A
PubMed ID18163486
'Mitochondrial membrane potential (Deltapsi) is key to mitochondrial function and cellular survival. Here, we aimed to develop an automated protocol allowing sensitive quantification of Deltapsi in living cells at the level of individual mitochondria. Human skin fibroblasts were stained with the fluorescent cation tetramethyl rhodamine methyl ester (TMRM), which is ... More
Mitochondrial dynamics and motility inside living vascular endothelial cells: role of bioenergetics.
AuthorsGiedt RJ, Pfeiffer DR, Matzavinos A, Kao CY, Alevriadou BR,
JournalAnn Biomed Eng
PubMed ID22527011
'The mitochondrial network is dynamic with conformations that vary between a tubular continuum and a fragmented state. The equilibrium between mitochondrial fusion/fission, as well as the organelle motility, determine network morphology and ultimately mitochondrial/cell function. Network morphology has been linked with the energy state in different cell types. In this ... More
NCX3 regulates mitochondrial calcium handling through AKAP121-anchored signaling complex and prevents hypoxia-induced cell death.
AuthorsScorziello A, Savoia C, Sisalli MJ, Adornetto A, Secondo A, Boscia F, Esposito A, Polishchuk EV, Polishchuk RS, Molinaro P, Carlucci A, Lignitto L, Di Renzo G, Feliciello A, Annunziato L,
Journal
PubMed ID24101730
The mitochondrial influx and efflux calcium pathways play a relevant role in cytosolic and mitochondrial calcium homeostasis and contribute to the regulation of mitochondrial functions in neurons. The mitochondrial Na(+)/Ca(2+) exchanger, although hypothesized in 1974, has been primarily investigated only from a functional point of view and its identity and ... More
An interaction between Bcl-xL and the voltage-dependent anion channel (VDAC) promotes mitochondrial Ca2+ uptake.
AuthorsHuang H, Hu X, Eno CO, Zhao G, Li C, White C,
Journal
PubMed ID23720737
The role of the antiapoptotic protein Bcl-xL in regulating mitochondrial Ca(2+) ([Ca(2+)]mito) handling was examined in wild-type (WT) and Bcl-xL knock-out (Bcl-xL-KO) mouse embryonic fibroblast cells. Inositol 1,4,5-trisphosphate-generating agonist evoked cytosolic Ca(2+) transients that produced a larger [Ca(2+)]mito uptake in WT cells compared with Bcl-xL-KO. In permeabilized cells, stepping external ... More
Human ALKBH7 is required for alkylation and oxidation-induced programmed necrosis.
AuthorsFu D, Jordan JJ, Samson LD,
Journal
PubMed ID23666923
Programmed necrosis has emerged as a crucial modulator of cell death in response to several forms of cellular stress. In one form of programmed necrotic cell death, induced by cytotoxic alkylating agents, hyperactivation of poly-ADP-ribose polymerase (PARP) leads to cellular NAD and ATP depletion, mitochondrial dysfunction, reactive oxygen species formation, ... More
Cytosolic caspases mediate mislocalised SOD2 depletion in an in vitro model of chronic prion infection.
AuthorsSinclair L, Lewis V, Collins SJ, Haigh CL,
JournalDis Model Mech
PubMed ID23580200
Oxidative stress as a contributor to neuronal death during prion infection is supported by the fact that various oxidative damage markers accumulate in the brain during the course of this disease. The normal cellular substrate of the causative agent, the prion protein, is also linked with protective functions against oxidative ... More
Tyrosine-phosphorylated caveolin-1 (Tyr-14) increases sensitivity to paclitaxel by inhibiting BCL2 and BCLxL proteins via c-Jun N-terminal kinase (JNK).
AuthorsShajahan AN, Dobbin ZC, Hickman FE, Dakshanamurthy S, Clarke R,
JournalJ Biol Chem
PubMed ID22433870
Paclitaxel, an anti-microtubule agent, is an effective chemotherapeutic drug in breast cancer. Nonetheless, resistance to paclitaxel remains a major clinical challenge. The need to better understand the resistant phenotype and to find biomarkers that could predict tumor response to paclitaxel is evident. In estrogen receptor a-positive (ER(+)) breast cancer cells, ... More
Distinct roles of mitochondria- and ER-localized Bcl-xL in apoptosis resistance and Ca2+ homeostasis.
AuthorsEno CO, Eckenrode EF, Olberding KE, Zhao G, White C, Li C,
JournalMol Biol Cell
PubMed ID22573883
Bcl-2 proteins are major regulators of cellular responses to intrinsic and extrinsic apoptotic stimuli. Among them, overexpression of the antiapoptotic protein Bcl-x(L) modulates intracellular Ca(2+) homeostasis and organelle-specific apoptotic signaling pathways. However, the specific activities of Bcl-x(L) at mitochondria and the endoplasmic reticulum (ER) have not been fully defined. To ... More
The mitochondrial protein C1qbp promotes cell proliferation, migration and resistance to cell death.
AuthorsMcGee AM, Douglas DL, Liang Y, Hyder SM, Baines CP,
JournalCell Cycle
PubMed ID22101277
Complement 1q-Binding Protein (C1qbp) is a mitochondrial protein reported to be upregulated in cancer. However, whether C1qbp plays a tumor suppressive or tumorigenic role in the progression of cancer is controversial. Moreover, the exact effects of C1qbp on cell proliferation, migration, and death/survival have not been definitely proven. To this ... More
Hemin causes mitochondrial dysfunction in endothelial cells through promoting lipid peroxidation: the protective role of autophagy.
AuthorsHigdon AN, Benavides GA, Chacko BK, Ouyang X, Johnson MS, Landar A, Zhang J, Darley-Usmar VM,
JournalAm J Physiol Heart Circ Physiol
PubMed ID22245770
The hemolysis of red blood cells and muscle damage results in the release of the heme proteins myoglobin, hemoglobin, and free heme into the vasculature. The mechanisms of heme toxicity are not clear but may involve lipid peroxidation, which we hypothesized would result in mitochondrial damage in endothelial cells. To ... More
Identifying and localizing intracellular nanoparticles using Raman spectroscopy.
AuthorsDorney J, Bonnier F, Garcia A, Casey A, Chambers G, Byrne HJ,
JournalAnalyst
PubMed ID22273712
Raman microscopy is employed to spectroscopically image biological cells previously exposed to fluorescently labelled polystyrene nanoparticles and, in combination with K-means clustering and principal component analysis (PCA), is demonstrated to be capable of localising the nanoparticles and identifying the subcellular environment based on the molecular spectroscopic signatures. The neutral nanoparticles ... More
Baculovirus-mediated gene transfer into mammalian cells.
AuthorsBoyce FM, Bucher NL,
JournalProc Natl Acad Sci U S A
PubMed ID8637876
This paper describes the use of the baculovirus Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) as a vector for gene delivery into mammalian cells. A modified AcMNPV virus was prepared that carried the Escherichia coli lacZ reporter gene under control of the Rous sarcoma virus promoter and mammalian RNA processing ... More
BacMam technology and its application to drug discovery.
AuthorsAmes RS, Kost TA, Condreay JP,
JournalExpert Opin Drug Discov
PubMed ID23488908
The recombinant baculovirus/insect cell system was firmly established as a leading method for recombinant protein production when a new potential use for these viruses was revealed in 1995. It was reported that engineered recombinant baculoviruses could deliver functional expression cassettes to mammalian cell types; a system which has come to ... More
Tools and techniques to measure mitophagy using fluorescence microscopy.
AuthorsDolman NJ, Chambers KM, Mandavilli B, Batchelor RH, Janes MS,
Journal
PubMed ID24121704
Mitophagy is a specialized form of autophagy that removes damaged mitochondria, thereby maintaining efficient cellular metabolism and reducing cellular stress caused by aberrant oxidative bursts. Deficits in mitophagy underlie several diseases, and a substantial body of research has elucidated key steps in the pathways that lead to and execute autophagic ... More
Encapsulated miR-200c and Nkx2.1 in a nuclear/mitochondria transcriptional regulatory network of non-metastatic and metastatic lung cancer cells.
AuthorsD'Almeida O, Mothar O, Bondzie EA, Lieumo Y, Tagne L, Gupta S, Volkert T, Levine S, Tagne JB
JournalBMC Cancer
PubMed ID30744585
'MicroRNAs are noncoding RNA molecules of ~?22 nucleotides with diagnostic and therapeutic action [Curr Drug Targets, 2015. 16(12): p. 1381-403], affecting the expression of mRNAs involved in invasion, migration, and development [Oncotarget, 2015. 6(9): p. 6472-98, Cancer Manag Res, 2014. 6: p. 205-16]. miR-200c is part of the miR-200c/141 cluster ... More
The novel cereblon modulator CC-885 inhibits mitophagy via selective degradation of BNIP3L.
AuthorsHao BB, Li XJ, Jia XL, Wang YX, Zhai LH, Li DZ, Liu J, Zhang D, Chen YL, Xu YH, Lee SK, Xu GF, Chen XH, Dang YJ, Liu B, Tan MJ
JournalActa Pharmacol Sin
PubMed ID32210356
'Mitophagy is a degradative pathway that mediates the degradation of the entire mitochondria, and defects in this process are implicated in many diseases including cancer. In mammals, mitophagy is mediated by BNIP3L (also known as NIX) that is a dual regulator of mitochondrial turnover and programmed cell death pathways. Acute ... More
Bcl-2-associated athanogene 5 (BAG5) regulates Parkin-dependent mitophagy and cell death.
AuthorsDe Snoo ML, Friesen EL, Zhang YT, Earnshaw R, Dorval G, Kapadia M, O'Hara DM, Agapova V, Chau H, Pellerito O, Tang MY, Wang X, Schmitt-Ulms G, Durcan TM, Fon EA, Kalia LV, Kalia SK
JournalCell Death Dis
PubMed ID31787745
'As pathogenic Parkin mutations result in the defective clearance of damaged mitochondria, Parkin-dependent mitophagy is thought to be protective against the dopaminergic neurodegeneration observed in Parkinson''s disease. Recent studies, however, have demonstrated that Parkin can promote cell death in the context of severe mitochondrial damage by degrading the pro-survival Bcl-2 ... More
Depletion of phosphatidylinositol 4-phosphate at the Golgi translocates K-Ras to mitochondria.
AuthorsMiller TE, Henkels KM, Huddleston M, Salisbury R, Hussain SM, Sasaki AT, Cho KJ
JournalJ Cell Sci
PubMed ID31331963
'Ras proteins are small GTPases localized to the plasma membrane (PM), which regulate cellular proliferation, apoptosis and differentiation. After a series of post-translational modifications, H-Ras and N-Ras traffic to the PM from the Golgi via the classical exocytic pathway, but the exact mechanism of K-Ras trafficking to the PM from ... More
Respiratory syncytial virus co-opts host mitochondrial function to favour infectious virus production.
Authors
JournalElife
PubMed ID31246170
NIK/MAP3K14 Regulates Mitochondrial Dynamics and Trafficking to Promote Cell Invasion.
Authors
JournalCurr Biol
PubMed ID27889261
Endothelial Stanniocalcin 1 Maintains Mitochondrial Bioenergetics and Prevents Oxidant-Induced Lung Injury via Toll-Like Receptor 4.
Authors
JournalAntioxid Redox Signal
PubMed ID30187766
Small Extracellular Vesicle Regulation of Mitochondrial Dynamics Reprograms a Hypoxic Tumor Microenvironment.
Authors
JournalDev Cell
PubMed ID32780991
Long-chain acyl-CoA synthetase 1 interacts with key proteins that activate and direct fatty acids into niche hepatic pathways.
Authors
JournalJ Biol Chem
PubMed ID30190326
Axonal generation of amyloid-β from palmitoylated APP in mitochondria-associated endoplasmic reticulum membranes.
Authors
JournalCell Rep
PubMed ID34010653
Human mesenchymal stromal cells transplanted into mice stimulate renal tubular cells and enhance mitochondrial function.
Authors
JournalNat Commun
PubMed ID29042548
Staurosporines disrupt phosphatidylserine trafficking and mislocalize Ras proteins.
Authors
JournalJ Biol Chem
PubMed ID23124205
Cycloheximide promotes paraptosis induced by inhibition of cyclophilins in glioblastoma multiforme.
Authors
JournalCell Death Dis
PubMed ID28518150
Dysregulation of mitochondria-lysosome contacts by GBA1 dysfunction in dopaminergic neuronal models of Parkinson's disease.
Authors
JournalNat Commun
PubMed ID33753743
Mitochondrial Neuroglobin Is Necessary for Protection Induced by Conditioned Medium from Human Adipose-Derived Mesenchymal Stem Cells in Astrocytic Cells Subjected to Scratch and Metabolic Injury.
AuthorsBaez-Jurado E, Guio-Vega G, Hidalgo-Lanussa O, González J, Echeverria V, Ashraf GM, Sahebkar A, Barreto GE
JournalMol Neurobiol
PubMed ID30536184
Astrocytes are specialized cells capable of regulating inflammatory responses in neurodegenerative diseases or traumatic brain injury. In addition to playing an important role in neuroinflammation, these cells regulate essential functions for the preservation of brain tissue. Therefore, the search for therapeutic alternatives to preserve these cells and maintain their functions ... More