Targeted cellular ablation based on the morphology of malignant cells.
AuthorsIvey JW, Latouche EL, Sano MB, Rossmeisl JH, Davalos RV, Verbridge SS
Journal
PubMed ID26596248
'Treatment of glioblastoma multiforme (GBM) is especially challenging due to a shortage of methods to preferentially target diffuse infiltrative cells, and therapy-resistant glioma stem cell populations. Here we report a physical treatment method based on electrical disruption of cells, whose action depends strongly on cellular morphology. Interestingly, numerical modeling suggests ... More
Fractional proliferation: a method to deconvolve cell population dynamics from single-cell data.
'We present an integrated method that uses extended time-lapse automated imaging to quantify the dynamics of cell proliferation. Cell counts are fit with a quiescence-growth model that estimates rates of cell division, entry into quiescence and death. The model is constrained with rates extracted experimentally from the behavior of tracked ... More
Analysis of nucleocytoplasmic transport using green fluorescent protein.
AuthorsStauber RH,
JournalMethods Mol Biol
PubMed ID12136753
A hallmark of eukaryotic cells is their spatial and functional separation into the nucleus and the cytoplasm by the nuclear envelope. Although this separation introduces a potent and sophisticated level of regulation, it also requires highly effective and selective transport machinery. ... More
Modulation of nucleocytoplasmic trafficking by retention in cytoplasm or nucleus.
AuthorsRoth DM, Harper I, Pouton CW, Jans DA,
JournalJ Cell Biochem
PubMed ID19507231
Nuclear protein transport processes have largely been studied using in vitro semi-intact cell systems where high concentrations of nuclear localizing substrates are used, and cytoplasmic components such as the microtubule (MT) network, are either absent or damaged. Here we use the fluorescence recovery after photobleaching (FRAP) technique to analyze the ... More
Assessment of GFP expression and viability using the tali image-based cytometer.
AuthorsRemple K, Stone L,
JournalJ Vis Exp
PubMed ID22127256
Single-cell and population information are commonly obtained either by flow cytometry or fluorescence microscopy. However, these two methods provide different information. Flow cytometry gives quantitative multi-parametric information about physical characteristics and staining or expression, but doesn't allow for visualization. Stand-alone fluorescence microscopy provides visual data, but doesn't allow for straightforward ... More
Baculovirus-mediated gene transfer into mammalian cells.
AuthorsBoyce FM, Bucher NL,
JournalProc Natl Acad Sci U S A
PubMed ID8637876
This paper describes the use of the baculovirus Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) as a vector for gene delivery into mammalian cells. A modified AcMNPV virus was prepared that carried the Escherichia coli lacZ reporter gene under control of the Rous sarcoma virus promoter and mammalian RNA processing ... More
BacMam technology and its application to drug discovery.
AuthorsAmes RS, Kost TA, Condreay JP,
JournalExpert Opin Drug Discov
PubMed ID23488908
The recombinant baculovirus/insect cell system was firmly established as a leading method for recombinant protein production when a new potential use for these viruses was revealed in 1995. It was reported that engineered recombinant baculoviruses could deliver functional expression cassettes to mammalian cell types; a system which has come to ... More
Single cell dynamic phenotyping.
Authors
JournalSci Rep
PubMed ID27708391
Adenoviral protein E4orf4 interacts with the polarity protein Par3 to induce nuclear rupture and tumor cell death.
Authors
JournalJ Cell Biol
PubMed ID32328642
Effects of cationic liposomes with stearylamine against virus infection.
AuthorsTahara K, Kobayashi M, Yoshida S, Onodera R, Inoue N, Takeuchi H
JournalInt J Pharm
PubMed ID29625169
In this study, we demonstrated that cationic liposomes with incorporated stearylamine (SA) inhibit viral infectivity without preloaded active pharmaceutical ingredients. Specifically, we correlated physiochemical properties of liposomes, such as zeta potentials and particle sizes, with virus infectivity using the BacMam™ reagent, which is based on recombinant baculovirus (BV). Compared with ... More
Physicochemical mechanotransduction alters nuclear shape and mechanics via heterochromatin formation.
AuthorsStephens AD, Liu PZ, Kandula V, Chen H, Almassalha LM, Herman C, Backman V, O'Halloran T, Adam SA, Goldman RD, Banigan EJ, Marko JF
JournalMol Biol Cell
PubMed ID31365328
The nucleus houses, organizes, and protects chromatin to ensure genome integrity and proper gene expression, but how the nucleus adapts mechanically to changes in the extracellular environment is poorly understood. Recent studies have revealed that extracellular physical stresses induce chromatin compaction via mechanotransductive processes. We report that increased extracellular multivalent ... More
Lactate Activates the E2F Pathway to Promote Cell Motility by Up-Regulating Microtubule Modulating Genes.
AuthorsJung YD, Cho JH, Park S, Kang M, Park SJ, Choi DH, Jeong M, Park KC, Yeom YI, Lee DC
JournalCancers (Basel)
PubMed ID30813560
Excess lactate production due to enhanced aerobic glycolysis is characteristic of malignant cancers, which is also intimately associated with poor cancer prognoses. Although tumor-associated lactate contributes to all major steps in carcinogenesis, its action mechanism remains obscure. To understand the molecular mechanism of the lactate-induced tumor metastatic process, we identified ... More