Relocalization of junctional adhesion molecule A during inflammatory stimulation of brain endothelial cells.
AuthorsStamatovic SM, Sladojevic N, Keep RF, Andjelkovic AV,
JournalMol Cell Biol
PubMed ID22733993
'Junctional adhesion molecule A (JAM-A) is a unique tight junction (TJ) transmembrane protein that under basal conditions maintains endothelial cell-cell interactions but under inflammatory conditions acts as a leukocyte adhesion molecule. This study investigates the fate of JAM-A during inflammatory TJ complex remodeling and paracellular route formation in brain endothelial ... More
Improved throughput traction microscopy reveals pivotal role for matrix stiffness in fibroblast contractility and TGF-ß responsiveness.
AuthorsMarinkovic A, Mih JD, Park JA, Liu F, Tschumperlin DJ,
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID22659883
'Lung fibroblast functions such as matrix remodeling and activation of latent transforming growth factor-ß1 (TGF-ß1) are associated with expression of the myofibroblast phenotype and are directly linked to fibroblast capacity to generate force and deform the extracellular matrix. However, the study of fibroblast force-generating capacities through methods such as traction ... More
Targeted detection of phosphatidylserine in biomimetic membranes and in vitro cell systems using annexin V-containing cubosomes.
AuthorsShen HH, Lake V, Le Brun AP, James M, Duff AP, Peng Y, McLean KM, Hartley PG,
Journal
PubMed ID23899446
'In this work we have formulated Annexin V (ANX) decorated phosphatidylserine containing phytantriol (PSPhy) cubosomes to act as probes for the enhanced detection of apoptotic membranes in both model and in vitro cell systems. Small angle X-ray scattering (SAXS) and cryogenic-transmission electron microscopy (Cryo-TEM) indicated that ANX-containing PSPhy (ANX-PSPhy) cubosomes ... More
Live cell imaging of G protein-coupled receptors.
AuthorsTeichmann A, Schmidt A, Wiesner B, Oksche A, Schülein R,
JournalMethods Mol Biol
PubMed ID22674164
Live cell imaging experiments with G protein-coupled receptors (GPCRs) tagged with fluorescent fusion proteins were originally performed to study trafficking and subcellular location of these important drug targets. In the past decade, however, substantial progress came from improved imaging methods and from the cloning of novel fluorescent fusion proteins. Today, ... More
Agonist leukadherin-1 increases CD11b/CD18-dependent adhesion via membrane tethers.
AuthorsCelik E, Faridi MH, Kumar V, Deep S, Moy VT, Gupta V,
Journal
PubMed ID24314082
Integrin CD11b/CD18 is a key adhesion receptor that mediates leukocyte migration and immune functions. Leukadherin-1 (LA1) is a small molecule agonist that enhances CD11b/CD18-dependent cell adhesion to its ligand ICAM-1. Here, we used single-molecule force spectroscopy to investigate the biophysical mechanism by which LA1-activated CD11b/CD18 mediates leukocyte adhesion. Between the ... More
Integrated multimodal optical microscopy for structural and functional imaging of engineered and natural skin.
AuthorsZhao Y, Graf BW, Chaney EJ, Mahmassani Z, Antoniadou E, Devolder R, Kong H, Boppart MD, Boppart SA,
JournalJ Biophotonics
PubMed ID22371330
An integrated multimodal optical microscope is demonstrated for high-resolution, structural and functional imaging of engineered and natural skin. This microscope incorporates multiple imaging modalities including optical coherence (OCM), multi-photon (MPM), and fluorescence lifetime imaging microscopy (FLIM), enabling simultaneous visualization of multiple contrast sources and mechanisms from cells and tissues. Spatially ... More
Baculovirus-mediated gene transfer into mammalian cells.
AuthorsBoyce FM, Bucher NL,
JournalProc Natl Acad Sci U S A
PubMed ID8637876
This paper describes the use of the baculovirus Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) as a vector for gene delivery into mammalian cells. A modified AcMNPV virus was prepared that carried the Escherichia coli lacZ reporter gene under control of the Rous sarcoma virus promoter and mammalian RNA processing ... More
BacMam technology and its application to drug discovery.
AuthorsAmes RS, Kost TA, Condreay JP,
JournalExpert Opin Drug Discov
PubMed ID23488908
The recombinant baculovirus/insect cell system was firmly established as a leading method for recombinant protein production when a new potential use for these viruses was revealed in 1995. It was reported that engineered recombinant baculoviruses could deliver functional expression cassettes to mammalian cell types; a system which has come to ... More
Novel elvitegravir nanoformulation for drug delivery across the blood-brain barrier to achieve HIV-1 suppression in the CNS macrophages.
AuthorsGong Y, Chowdhury P, Nagesh PKB, Rahman MA, Zhi K, Yallapu MM, Kumar S
JournalSci Rep
PubMed ID32123217
The use of antiretroviral therapy (ART) has remarkably decreased the morbidity associated with HIV-1 infection, however, the prevalence of HIV-1-associated neurocognitive disorders (HAND) is still increasing. The blood-brain barrier (BBB) is the major impediment for penetration of antiretroviral drugs, causing therapeutics to reach only suboptimal level to the brain. Conventional ... More
Avicin G is a potent sphingomyelinase inhibitor and blocks oncogenic K- and H-Ras signaling.
AuthorsGarrido CM, Henkels KM, Rehl KM, Liang H, Zhou Y, Gutterman JU, Cho KJ
JournalSci Rep
PubMed ID32499517
K-Ras must interact primarily with the plasma membrane (PM) for its biological activity. Therefore, disrupting K-Ras PM interaction is a tractable approach to block oncogenic K-Ras activity. Here, we found that avicin G, a family of natural plant-derived triterpenoid saponins from Acacia victoriae, mislocalizes K-Ras from the PM and disrupts ... More