A portable BRCA1-HAC (human artificial chromosome) module for analysis of BRCA1 tumor suppressor function.
AuthorsKononenko AV, Bansal R, Lee NC, Grimes BR, Masumoto H, Earnshaw WC, Larionov V, Kouprina N,
Journal
PubMed ID25260588
'BRCA1 is involved in many disparate cellular functions, including DNA damage repair, cell-cycle checkpoint activation, gene transcriptional regulation, DNA replication, centrosome function and others. The majority of evidence strongly favors the maintenance of genomic integrity as a principal tumor suppressor activity of BRCA1. At the same time some functional aspects ... More
The microanatomic segregation of selection by apoptosis in the germinal center.
AuthorsMayer CT, Gazumyan A, Kara EE, Gitlin AD, Golijanin J, Viant C, Pai J, Oliveira TY, Wang Q, Escolano A, Medina-Ramirez M, Sanders RW, Nussenzweig MC,
JournalScience
PubMed ID28935768
'B cells undergo rapid cell division and affinity maturation in anatomically distinct sites in lymphoid organs called germinal centers (GCs). Homeostasis is maintained in part by B cell apoptosis. However, the precise contribution of apoptosis to GC biology and selection is not well defined. We developed apoptosis-indicator mice and used ... More
BRCA2 suppresses replication stress-induced mitotic and G1 abnormalities through homologous recombination.
AuthorsFeng W, Jasin M,
JournalNat Commun
PubMed ID28904335
Mutations in the tumor suppressor BRCA2 predominantly predispose to breast cancer. Paradoxically, while loss of BRCA2 promotes tumor formation, it also causes cell lethality, although how lethality is triggered is unclear. Here, we generate BRCA2 conditional non-transformed human mammary epithelial cell lines using CRISPR-Cas9. Cells are inviable upon BRCA2 loss, ... More
Pulmonary pericytes regulate lung morphogenesis.
AuthorsKato K, Diéguez-Hurtado R, Park DY, Hong SP, Kato-Azuma S, Adams S, Stehling M, Trappmann B, Wrana JL, Koh GY, Adams RH,
JournalNat Commun
PubMed ID29934496
Blood vessels are essential for blood circulation but also control organ growth, homeostasis, and regeneration, which has been attributed to the release of paracrine signals by endothelial cells. Endothelial tubules are associated with specialised mesenchymal cells, termed pericytes, which help to maintain vessel wall integrity. Here we identify pericytes as ... More
Single-cell transcriptomics reveals a new dynamical function of transcription factors during embryonic hematopoiesis.
AuthorsBergiers I, Andrews T, Vargel Bölükbasi Ö, Buness A, Janosz E, Lopez-Anguita N, Ganter K, Kosim K, Celen C, Itir Perçin G, Collier P, Baying B, Benes V, Hemberg M, Lancrin C,
JournalElife
PubMed ID29555020
Recent advances in single-cell transcriptomics techniques have opened the door to the study of gene regulatory networks (GRNs) at the single-cell level. Here, we studied the GRNs controlling the emergence of hematopoietic stem and progenitor cells from mouse embryonic endothelium using a combination of single-cell transcriptome assays. We found that ... More