'ADP-ribosylation factor (Arf) 6 regulates the movement of membrane between the plasma membrane (PM) and a nonclathrin-derived endosomal compartment and activates phosphatidylinositol 4-phosphate 5-kinase (PIP 5-kinase), an enzyme that generates phosphatidylinositol 4,5-bisphosphate (PIP2). Here, we show that PIP2 visualized by expressing a fusion protein of the pleckstrin homology domain from ... More
Expression of UME6, a key regulator of Candida albicans hyphal development, enhances biofilm formation via Hgc1- and Sun41-dependent mechanisms.
AuthorsBanerjee M, Uppuluri P, Zhao XR, Carlisle PL, Vipulanandan G, Villar CC, López-Ribot JL, Kadosh D,
JournalEukaryot Cell
PubMed ID23223035
'Biofilm formation is associated with the ability of Candida albicans, the major human fungal pathogen, to resist antifungal therapies and grow on tissues, catheters, and medical devices. In order to better understand the relationship between C. albicans morphology and biofilm formation, we examined biofilms generated in response to expression of ... More
G proteins mediate changes in cell shape by stabilizing the axis of polarity.
AuthorsNern A, Arkowitz RA
JournalMol Cell
PubMed ID10882121
Upon exposure to mating pheromone, yeast cells change their form to pear-shaped shmoos. We looked at pheromone-dependent cell shape changes in mutants that are unable to orient growth during mating and unable to choose a bud site. In these double mutants, cell surface growth, secretion sites, cytoskeleton, and pheromone receptors ... More
Environmental hyperosmolality regulates phospholipid biosynthesis in the renal epithelial cell line MDCK.
AuthorsCasali CI, Weber K, Favale NO, Tome MC,
JournalJ Lipid Res
PubMed ID23269393
Hyperosmolality is a key signal for renal physiology. On the one hand, it contributes to the differentiation of renal medullary structures and to the development of the urinary concentrating mechanism. On the other, it is a stress factor. In both cases, hyperosmolality activates processes that require an adequate extension of ... More
Visualization in zebrafish larvae of Na(+) uptake in mitochondria-rich cells whose differentiation is dependent on foxi3a.
Uptake of Na(+) from the environment is an indispensable strategy for the survival of freshwater fish, as they easily lose Na(+) from the plasma to a diluted environment. Nevertheless, the location of and molecules involved in Na(+) uptake remain poorly understood. In this study, we utilized Sodium Green, a Na(+)-dependent ... More
Fluorescence visualization of branchial collagen columns embraced by pillar cells.
AuthorsKudo H, Kato A, Hirose S
JournalJ Histochem Cytochem
PubMed ID16957162
A collagen column is a structure of the extracellular matrix that helps to maintain the flatness and width of gill lamella. Collagen columns are unique in that they are enfolded by plasma membrane of pillar cells that form two-dimensional vascular networks between parallel sheets of respiratory epithelia. Despite their unique ... More
Intracellular and extracellular leukemia inhibitory factor proteins have different cellular activities that are mediated by distinct protein motifs.
AuthorsHaines BP, Voyle RB, Rathjen PD
JournalMol Biol Cell
PubMed ID10749936
Although many growth factors and cytokines have been shown to be localized within the cell and nucleus, the mechanism by which these molecules elicit a biological response is not well understood. The cytokine leukemia inhibitory factor (LIF) provides a tractable experimental system to investigate this problem, because translation of alternatively ... More
The plane of first cleavage is not related to the distribution of sperm components in the mouse.
AuthorsDavies TJ, Gardner RL
JournalHum Reprod
PubMed ID12202427
BACKGROUND: Marking experiments using phytohaemagglutinin (PHA) and Concanavalin A (ConA) have suggested that the first cleavage plane is related to the point of sperm entry. Because of concerns about the specificity of lectin binding, the distribution of sperm components has been investigated directly. METHODS AND RESULTS: The sperm tail could ... More
Control of intracellular movement of connexins by E-cadherin in murine skin papilloma cells.
AuthorsHernandez-Blazquez FJ, Joazeiro PP, Omori Y, Yamasaki H
JournalExp Cell Res
PubMed ID11640887
The gap junctional intercellular communication-deficient mouse skin papilloma cell line P3/22 expresses Cx43 but not E-cadherin. The E-cadherin gene-transfected cells (P3E1) communicate in a calcium-dependent manner and they were used to study how E-cadherin restores the function of connexins. At low calcium, Cx43 molecules remain in the cytoplasm of P3E1 ... More
Elements in the N-terminal signaling sequence that determine cytosolic topology of short-chain dehydrogenases/reductases. Studies with retinol dehydrogenase type 1 and cis-retinol/androgen dehydrogenase type 1.
AuthorsZhang M, Hu P, Napoli JL
JournalJ Biol Chem
PubMed ID15355969
High affinity, retinoid-specific binding proteins chaperone retinoids to manage their transport and metabolism. Proposing mechanisms of retinoid transfer between these binding proteins and membrane-associated retinoid-metabolizing enzymes requires insight into enzyme topology. We therefore determined the topology of mouse retinol dehydrogenase type 1 (Rdh1) and cis-retinoid androgen dehydrogenase type 1 (Crad1) ... More
Yeast species-specific, differential inhibition of β-1,3-glucan synthesis by poacic acid and caspofungin.
Authors
JournalCell Surf
PubMed ID30370375
The tricellular vertex-specific adhesion molecule Sidekick facilitates polarised cell intercalation during Drosophila axis extension.
Authors
JournalPLoS Biol
PubMed ID31805038
Specific mechanism of Acidithiobacillus caldus extracellular polymeric substances in the bioleaching of copper-bearing sulfide ore.
Authors
JournalPLoS One
PubMed ID30978195
Genetic control of conventional and pheromone-stimulated biofilm formation in Candida albicans.
Authors
JournalPLoS Pathog
PubMed ID23637598
CD301 mediates fusion in IL-4-driven multinucleated giant cell formation.
Authors
JournalJ Cell Sci
PubMed ID33571108
ATG24 Represses Autophagy and Differentiation and Is Essential for Homeostasy of the Flagellar Pocket in Trypanosoma brucei.
Authors
JournalPLoS One
PubMed ID26090847
An expanded regulatory network temporally controls Candida albicans biofilm formation.
Authors
JournalMol Microbiol
PubMed ID25784162
A kinase inhibitor screen reveals protein kinase C-dependent endocytic recycling of ErbB2 in breast cancer cells.
Authors
JournalJ Biol Chem
PubMed ID25225290
The packing density of a supramolecular membrane protein cluster is controlled by cytoplasmic interactions.
Authors
JournalElife
PubMed ID28722652
Localization to the cortical cytoskeleton is necessary for Nf2/merlin-dependent epidermal growth factor receptor silencing.