Dulbecco's Modified Eagle Medium
: Nutrient Mixture F-12 (DMEM/F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts.
This DMEM / F-12 is modified as follows:
DMEM/F-12 is a 1
:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10%
Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system (2.438 g/L) and therefore requires a 5-10% CO
2 environment to maintain physiological pH.
Gibco® 'BASIC' products offer value-minded scientists liquid cell culture media and buffer solutions at a significant price savings. As part of Life Technologies’ commitment to quality, this select range of Research Use Only products is continually tested to ensure performance and customer satisfaction.
For Research Use Only. Not for use in diagnostic procedures.