Live-cell imaging of rhabdovirus-induced morphological changes in plant nuclear membranes.
AuthorsGoodin M, Yelton S, Ghosh D, Mathews S, Lesnaw J
JournalMol Plant Microbe Interact
PubMed ID16042016
'Potato yellow dwarf virus (PYDV) and Sonchus yellow net virus (SYNV) belong to the genus Nucleorhabdovirus. These viruses replicate in nuclei of infected cells and mature virions accumulate in the perinuclear space after budding through the inner nuclear membrane. Infection of transgenic Nicotiana benthamiana 16c plants (which constitutively express green ... More
High-density polymerase-mediated incorporation of fluorochrome-labeled nucleotides.
AuthorsRamanathan A, Pape L, Schwartz DC
JournalAnal Biochem
PubMed ID15649370
'DNA-polymerase-mediated incorporation of different fluorochrome-labeled nucleotides (FdNTPs) was investigated with the goals of optimizing the high-density labeling of probes and exploring DNA sequencing strategies that rely on the controlled, sequential addition of such compounds. By systematically evaluating variables--including polymerase type, buffer conditions, and fluorochrome chemistries--a rational strategy for the sequential ... More
Determination of surface-bound-fluorophore orientation by goniometric fluorescence polarization: application to quantification of DNA-chip readouts.
AuthorsBarritault P, Gétin S, Chaton P, Vinet F, Fouqué B
JournalAppl Opt
PubMed ID12153110
We present a polarized goniofluorimeter designed to measure the observation-angle and polarization-dependent intensity emitted by a group of surface-bound fluorescent molecules. We studied two types of surface bonding: In one case, dyes were adsorbed into the surface by spin coating, and in the other, dyes were covalently immobilized to DNA ... More
Maximization of signal derived from cDNA microarrays.
AuthorsWildsmith SE, Archer GE, Winkley AJ, Lane PW, Bugelski PJ
JournalBiotechniques
PubMed ID11196312
Microarray technology is a powerful tool for generating expression data on a large number of genes simultaneously. However, as for any assay, it must be reproducible to give confidence in the results. Using a classical statistical method--the factorial design of experiments--we have assessed the effects of different experimental factors in ... More
The positioning and dynamics of origins of replication in the budding yeast nucleus.
AuthorsHeun P, Laroche T, Raghuraman MK, Gasser SM
JournalJ Cell Biol
PubMed ID11266454
We have analyzed the subnuclear position of early- and late-firing origins of DNA replication in intact yeast cells using fluorescence in situ hybridization and green fluorescent protein (GFP)-tagged chromosomal domains. In both cases, origin position was determined with respect to the nuclear envelope, as identified by nuclear pore staining or ... More