JournalJournal of immunology (Baltimore, Md. : 1950)
PubMed ID17548610
Calcein: a novel marker for lymphocytes which enter lymph nodes.
AuthorsWeston SA, Parish CR
JournalCytometry
PubMed ID1451604
Previous studies have identified unique cell surface antigens which are associated with the specific binding of lymphocytes to high endothelial venules (HEV). Evidence is presented in this paper which demonstrates that uptake of the fluorescent dye calcein by lymphocytes represents an additional marker for the lymph node homing subpopulation of ... More
Platelet-endothelial interaction in tumor angiogenesis and microcirculation.
AuthorsManegold PC, Hutter J, Pahernik SA, Messmer K, Dellian M
JournalBlood
PubMed ID12584142
Activated platelets release angiogenic growth factors and have therefore been proposed to contribute to tumor angiogenesis within a potentially prothrombotic tumor microcirculation. The aim of the study was to investigate interactions of platelets with the angiogenic microvascular endothelium of highly vascularized solid tumors during growth and in response to endothelial ... More
Primitive, quiescent, Philadelphia-positive stem cells from patients with chronic myeloid leukemia are insensitive to STI571 in vitro.
AuthorsGraham SM, Jørgensen HG, Allan E, Pearson C, Alcorn MJ, Richmond L, Holyoake TL
JournalBlood
PubMed ID11756187
In clinical trials, the tyrosine kinase inhibitor STI571 has proven highly effective in reducing leukemic cell burden in chronic myeloid leukemia (CML). The overall sensitivity of CML CD34(+) progenitor cells to STI571 and the degree to which cell death was dependent on cell cycle status were determined. Stem cells (Lin(-)CD34(+)) ... More
Gap junctional communication and connexin43 expression in relation to apoptotic cell death and survival of granulosa cells.
AuthorsKrysko DV, Mussche S, Leybaert L, D'Herde K
JournalJ Histochem Cytochem
PubMed ID15314087
Ovarian follicular atresia in all vertebrates is mediated via apoptosis that is initiated in the granulosa cell layer. Here we investigated the relation between connexin expression, cell coupling, and apoptosis in avian granulosa cells. Results from qualitative and quantitative immunocytochemical analysis and Western blotting of connexin43 (Cx43) and electron microscopic ... More
A single class II myosin modulates T cell motility and stopping, but not synapse formation.
AuthorsJacobelli J, Chmura SA, Buxton DB, Davis MM, Krummel MF
JournalNat Immunol
PubMed ID15064761
'Upon encountering an antigen, motile T cells stop crawling, change morphology and ultimately form an ''immunological synapse''. Although myosin motors are thought to mediate various aspects of this process, the molecules involved and their exact roles are not defined. Here we show that nonmuscle myosin heavy chain IIA, or MyH9, ... More
Direct priming of antiviral CD8+ T cells in the peripheral interfollicular region of lymph nodes.
'It is uncertain how antiviral lymphocytes are activated in draining lymph nodes, the site where adaptive immune responses are initiated. Here, using intravital microscopy we show that after infection of mice with vaccinia virus (a large DNA virus) or vesicular stomatitis virus (a small RNA virus), virions drained to the ... More
Beta cells cannot directly prime diabetogenic CD8 T cells in nonobese diabetic mice.
Authorsde Jersey J, Snelgrove SL, Palmer SE, Teteris SA, Mullbacher A, Miller JF, Slattery RM
JournalProc Natl Acad Sci U S A
PubMed ID17229843
'Type 1 diabetes (T1D) is caused by the destruction of insulin-producing islet beta cells. CD8 T cells are prevalent in the islets of T1D patients and are the major effectors of beta cell destruction in nonobese diabetic (NOD) mice. In addition to their critical involvement in the late stages of ... More
Down-regulation of diabetogenic CD4+ T cells by a soluble dimeric peptide-MHC class II chimera.
AuthorsCasares S, Hurtado A, McEvoy RC, Sarukhan A, von Boehmer H, Brumeanu TD
JournalNat Immunol
PubMed ID11862219
'Type 1 diabetes is an organ-specific autoimmune disease that is mediated by autoreactive T cells. We show here that administration of a soluble dimeric peptide-major histocompatibility complex (pMHC) class II chimera (DEF) to prediabetic double-transgenic mice prevents the onset of disease or, in animals that are already diabetic, restores normoglycemia. ... More
Interleukin-6 is crucial for recall of influenza-specific memory CD4 T cells.
AuthorsLonghi MP, Wright K, Lauder SN, Nowell MA, Jones GW, Godkin AJ, Jones SA, Gallimore AM,
JournalPLoS Pathog
PubMed ID18389078
'Currently, our understanding of mechanisms underlying cell-mediated immunity and particularly of mechanisms that promote robust T cell memory to respiratory viruses is incomplete. Interleukin (IL)-6 has recently re-emerged as an important regulator of T cell proliferation and survival. Since IL-6 is abundant following infection with influenza virus, we analyzed virus-specific ... More
Profiling antibody responses by multiparametric analysis of primary B cells.
AuthorsStory CM, Papa E, Hu CC, Ronan JL, Herlihy K, Ploegh HL, Love JC,
JournalProc Natl Acad Sci U S A
PubMed ID19004776
'Determining the efficacy of a vaccine generally relies on measuring neutralizing antibodies in sera. This measure cannot elucidate the mechanisms responsible for the development of immunological memory at the cellular level, however. Quantitative profiles that detail the cellular origin, extent, and diversity of the humoral (antibody-based) immune response would improve ... More
Extracellular RNA constitutes a natural procoagulant cofactor in blood coagulation.
AuthorsKannemeier C, Shibamiya A, Nakazawa F, Trusheim H, Ruppert C, Markart P, Song Y, Tzima E, Kennerknecht E, Niepmann M, von Bruehl ML, Sedding D, Massberg S, Günther A, Engelmann B, Preissner KT
JournalProc Natl Acad Sci U S A
PubMed ID17405864
'Upon vascular injury, locally controlled haemostasis prevents life-threatening blood loss and ensures wound healing. Intracellular material derived from damaged cells at these sites will become exposed to blood components and could contribute to blood coagulation and pathological thrombus formation. So far, the functional and mechanistic consequences of this concept are ... More
An impaired transendothelial migration potential of chronic lymphocytic leukemia (CLL) cells can be linked to ephrin-A4 expression.
AuthorsTrinidad EM, Ballesteros M, Zuloaga J, Zapata A, Alonso-Colmenar LM,
JournalBlood
PubMed ID19828693
'Chronic lymphocytic leukemia (CLL) cell migration into lymphoid tissues is an important aspect of the pathobiology of this disease. Here, we investigated the role of ephrin-A4 (EFNA4) in the transendothelial migration (TEM) capacity of CLL and normal B cells through interacting with endothelial EphA2 (erythropoietin-producing hepatocellular carcinoma). CLL cells showed ... More
Augmentation in expression of activation-induced genes differentiates memory from naive CD4+ T cells and is a molecular mechanism for enhanced cellular response of memory CD4+ T cells.
AuthorsLiu K, Li Y, Prabhu V, Young L, Becker KG, Munson PJ, Weng Np
JournalJ Immunol
PubMed ID11390484
'In an attempt to understand the molecular basis for the immunological memory response, we have used cDNA microarrays to measure gene expression of human memory and naive CD4+ T cells at rest and after activation. Our analysis of 54,768 cDNA clones provides the first glimpse into gene expression patterns of ... More
Toward the generation of rod and cone photoreceptors from mouse, monkey and human embryonic stem cells.
AuthorsOsakada F, Ikeda H, Mandai M, Wataya T, Watanabe K, Yoshimura N, Akaike A, Akaike A, Sasai Y, Takahashi M,
JournalNat Biotechnol
PubMed ID18246062
'We previously reported the differentiation of mouse embryonic stem (ES) cells into retinal progenitors. However, these progenitors rarely differentiate into photoreceptors unless they are cultured with embryonic retinal tissues. Here we show the in vitro generation of putative rod and cone photoreceptors from mouse, monkey and human ES cells by ... More
CD133+ renal progenitor cells contribute to tumor angiogenesis.
'In the present study, we tested the hypothesis that resident progenitor cells may contribute to tumor vascularization and growth. CD133+ cells were isolated from 30 human renal carcinomas and characterized as renal resident progenitor cells on the basis of the expression of renal embryonic and mesenchymal stem cell markers. CD133+ ... More
Direct recognition of foreign MHC determinants by naive T cells mobilizes specific Vbeta families without skewing of the complementarity-determining region 3 length distribution.
AuthorsSebille F, Gagne K, Guillet M, Degauque N, Pallier A, Brouard S, Vanhove B, Delsuc MA, Soulillou JP
JournalJ Immunol
PubMed ID11544292
'The capacity of T cells to interact with nonself-APC, also referred to as direct allorecognition, is an essential feature of the cellular response involved in graft rejection. However, there is no study on TCR repertoire biases associated with direct restricted T cell activation. In this paper, we have addressed the ... More
Histological identification of purified and cryopreserved allogeneic hepatocytes following transplantation in a murine model without host immunosuppression.
AuthorsOstrowska A, Karrer FM, Bilir BM
JournalTranspl Int
PubMed ID10429956
'Hepatocyte transplantation is a conceptually attractive alternative to whole organ grafting for some inborn metabolic errors and for fulminant liver failure. However, studies of the immunogenicity of transplanted allogeneic hepatocytes have yielded contradictory results. In these experiments, the effect of purification and cryopreservation of the hepatocytes on the ability of ... More
Blood-brain barrier permeability precedes senile plaque formation in an Alzheimer disease model.
AuthorsUjiie M, Dickstein DL, Carlow DA, Jefferies WA
JournalMicrocirculation
PubMed ID14745459
'OBJECTIVE: To establish the generality of cerebrovascular pathology frequently observed with Alzheimer disease, we have assessed blood-brain barrier (BBB) integrity using the Alzheimer disease model Tg2576 mice in which cognitive deficits and neuritic plaque formation develop around 10-12 months of age. METHODS: We assessed BBB integrity using well-established methods involving ... More
Immunoneutralization of glycoprotein Ibalpha attenuates endotoxin-induced interactions of platelets and leukocytes with rat venular endothelium in vivo.
AuthorsKatayama T, Ikeda Y, Handa M, Tamatani T, Sakamoto S, Ito M, Ishimura Y, Suematsu M
JournalCirc Res
PubMed ID10827132
'This study aimed to examine molecular mechanisms for endotoxin-induced adhesive changes in platelets in vivo. Platelets labeled with carboxyfluorescein diacetate succinimidyl ester were visualized in rat mesenteric venules through intravital microscopy assisted by a high-speed fluorescence video imager at 1000 frames per second or by a normal-speed intensifier under monitoring ... More
In vivo cross-presentation of a soluble protein antigen: kinetics, distribution, and generation of effector CTL recognizing dominant and subdominant epitopes.
AuthorsNelson D, Bundell C, Robinson B
JournalJ Immunol
PubMed ID11086045
'Cross-presentation of exogenous Ags via the MHC class I pathway is now recognized for its role in self-tolerance, tumor immunity, and vaccine development. However, little is known about the in vivo distribution and kinetics of cross-presented protein Ags, nor the subsequent development of CTL effector responses to dominant or subdominant ... More
Enteric infection acts as an adjuvant for the response to a model food antigen.
AuthorsShi HN, Liu HY, Nagler-Anderson C
JournalJ Immunol
PubMed ID11086051
'Oral administration of soluble protein Ags typically induces Ag-specific systemic nonresponsiveness. However, we have found that feeding a model food protein, OVA, to helminth-infected mice primes for a systemic OVA-specific Th2 response. In this report we show that, in addition to creating a Th2-priming cytokine environment, helminth infection up-regulates costimulatory ... More
Anatomical and physiological localization of prelabeled grafts in rat hippocampus.
AuthorsPyapali GK, Turner DA, Madison RD
JournalExp Neurol
PubMed ID1577121
'Dissociated rat fetal hippocampal cells were grafted into normal adult rats. The fetal cells were incubated with one of a number of fluorescent compounds at the time of the dissociation to facilitate identification of the individual grafted cells. The fluorescent labels which were analyzed for this purpose included rhodamine latex ... More
Quantifying the frequency of alloreactive T cells in vivo: new answers to an old question.
AuthorsSuchin EJ, Langmuir PB, Palmer E, Sayegh MH, Wells AD, Turka LA
JournalJ Immunol
PubMed ID11145675
'Alloreactive T cell precursor frequency was measured in vivo using fluorescent dye labeling in combination with novel models based on lymphocyte activation and recovery. CFSE-labeled C57BL/6 (H-2(b)) spleen and lymph node cells were adoptively transferred to C57BL/6xDBA F(1) (H-2(b/d)) recipients, a parent-->F(1) MHC mismatch in which only donor cells respond. ... More
Use of nanogold- and fluorescent-labeled antibody Fv fragments in immunocytochemistry.
AuthorsRibrioux S, Kleymann G, Haase W, Heitmann K, Ostermeier C, Michel H
JournalJ Histochem Cytochem
PubMed ID8648079
'Recombinant antibody fragments are emerging as a versatile tool in both basic research and medical therapy. We describe the procedures for direct labeling of engineered antibody fragments (Fv) with fluorescein or nanogold and their use in fluorescence and immunoelectron microscopy, respectively. The Fv fragments were produced in Escherichia coli, purified ... More
Adaptive tolerance of CD4+ T cells in vivo: multiple thresholds in response to a constant level of antigen presentation.
'The in vivo T cell response to persistent Ag contains a hyporesponsive phase following an initial expansion and subsequent partial deletion of the responding cells. The mechanism(s) responsible for this tolerance process is poorly understood. In this study, we describe a new paired transgenic model (TCR and Ag), which within ... More
Activation of endogenous thrombin receptors causes clustering and sensitization of epidermal growth factor receptors of swiss 3T3 cells without transactivation.
AuthorsCrouch MF, Davy DA, Willard FS, Berven LA
JournalJ Cell Biol
PubMed ID11266444
'The G protein-coupled thrombin receptor can induce cellular responses in some systems by transactivating the epidermal growth factor (EGF) receptor. This is in part due to the stimulation of ectoproteases that generate EGF receptor ligands. We show here that this cannot account for the stimulation of proliferation or migration by ... More
Isolation and characterization of primordial follicles from fresh and cryopreserved human ovarian tissue.
AuthorsOktay K, Nugent D, Newton H, Salha O, Chatterjee P, Gosden RG
JournalFertil Steril
PubMed ID9091334
'OBJECTIVE: To develop an efficient isolation technique for human primordial follicles. DESIGN: Prospective, experimental study of ovarian biopsies collected from healthy women undergoing elective cesarean section. Ovarian blocks either were fixed for histology and follicle counting or partially disaggregated with type 1A collagenase before or after cryopreservation. After partial disaggregation, ... More
Vasoactive intestinal peptide modulates T lymphocyte migration in Peyer's patches of rat small intestine.
AuthorsMiura S, Serizawa H, Tsuzuki Y, Kurose I, Suematsu M, Higuchi H, Shigematsu T, Hokari R, Hirokawa M, Kimura H, Ishii H
JournalAm J Physiol
PubMed ID9038881
'Although vasoactive intestinal peptide (VIP) has been postulated to function in modulation of T cell trafficking, the exact mechanism has not been elucidated in vivo. In the present study, the effects of VIP on T lymphocyte migration were examined in rat Peyer''s patches. T lymphocytes collected from intestinal lymph of ... More
Cell proximity is a prerequisite for the proliferative response of bystander cells co-cultured with cells irradiated with gamma-rays.
AuthorsGerashchenko BI, Howell RW
JournalCytometry A
PubMed ID14608634
'BACKGROUND: In a recent study, we showed that unirradiated cells, when they are in the presence of cells irradiated with gamma-rays, are characterized by enhanced cell growth (Cytometry 2003;54A:1-7). However, the mechanisms and factors involved in the proliferative response of bystander cells are largely unknown. The aim of the current ... More
Latent HIV-1 reactivation in transgenic mice requires cell cycle -dependent demethylation of CREB/ATF sites in the LTR.
'OBJECTIVE: We previously produced a line of transgenic mice that carried the HIV-1 genome deficient in the gene. Although the HIV-1 genome in the lymphocytes was dormant under normal physiological conditions, it could be reactivated by lipopolysaccharide (LPS) administration via induction of interleukin-1alpha/beta and tumour necrosis factor-alpha. In this report, ... More
Effects of surface coordination chemistry on the magnetic properties of MnFe(2)O(4) spinel ferrite nanoparticles.
AuthorsVestal CR, Zhang ZJ
JournalJ Am Chem Soc
PubMed ID12904049
'To understand the influence of surface interactions upon the magnetic properties of magnetic nanoparticles, the surface of manganese ferrite, MnFe(2)O(4), nanoparticles have been systematically modified with a series of para-substituted benzoic acid ligands (HOOC-C(6)H(4)-R; R = H, CH(3), Cl, NO(2), OH) and substituted benzene ligands (Y-C(6)H(5), Y = COOH, SH, ... More
SLAT regulates Th1 and Th2 inflammatory responses by controlling Ca2+/NFAT signaling.
AuthorsBécart S, Charvet C, Canonigo Balancio AJ, De Trez C, Tanaka Y, Duan W, Ware C, Croft M, Altman A
JournalJ Clin Invest
PubMed ID17657315
'SWAP-70-like adapter of T cells (SLAT) is a novel guanine nucleotide exchange factor for Rho GTPases that is upregulated in Th2 cells, but whose physiological function is unclear. We show that SLAT(-/-) mice displayed a developmental defect at one of the earliest stages of thymocyte differentiation, the double-negative 1 (DN1) ... More
Flow cytometric analysis of fluorescence in situ hybridization with dye dilution and DNA staining (flow-FISH-DDD) to determine telomere length dynamics in proliferating cells.
AuthorsPotter AJ, Wener MH
JournalCytometry A
PubMed ID16163702
'BACKGROUND: Telomeres shorten during DNA replication; extensive erosion of telomeres likely promotes replicative senescence and chromosomal instability. Telomere length in individual cells has been quantified by flow cytometric analysis of fluorescence in situ hybridization (flow-FISH). To determine the rate of telomere attrition (telomere erosion per cell division), we combined flow-FISH ... More
A novel cadherin cell adhesion molecule: its expression patterns associated with implantation and organogenesis of mouse embryos.
AuthorsNose A, Takeichi M
JournalJ Cell Biol
PubMed ID3539943
'The Ca2+-dependent cell adhesion molecules, termed cadherins, were previously divided into two subclasses, E- and N-types, with different adhesive specificity. In this study, we identified a novel class of cadherin, termed P-cadherin, using a visceral endoderm cell line PSA5-E. This cadherin was a 118,000-D glycoprotein and distinct from E- and ... More
Sustained expression of CD154 (CD40L) and proinflammatory cytokine production by alloantigen-stimulated umbilical cord blood T cells.
AuthorsMatthews NC, Wadhwa M, Bird C, Borras FE, Navarrete CV
JournalJ Immunol
PubMed ID10843672
'Recent data suggests that graft-versus-host disease (GVHD) is initiated by host APCs. Blockade of CD40:CD154 interactions between APCs and T cells in vivo induces T cell tolerance to host alloantigen and dramatically reduces GVHD. Because allogeneic cord blood (CB) transplantation results in a lower incidence and severity of acute GVHD ... More
Groucho/transducin-like Enhancer-of-split (TLE)-dependent and -independent transcriptional regulation by Runx3.
AuthorsYarmus M, Woolf E, Bernstein Y, Fainaru O, Negreanu V, Levanon D, Groner Y
JournalProc Natl Acad Sci U S A
PubMed ID16651517
'Regulation of gene expression by tissue-specific transcription factors involves both turning on and turning off transcription of target genes. Runx3, a runt-domain transcription factor, regulates cell-intrinsic functions by activating and repressing gene expression in sensory neurons, dendritic cells (DC), and T cells. To investigate the mechanism of Runx3-mediated repression in ... More
A comparative study of carboxyfluorescein diacetate and carboxyfluorescein diacetate succinimidyl ester as indicators of bacterial activity.
AuthorsHoefel D, Grooby WL, Monis PT, Andrews S, Saint CP
JournalJ Microbiol Methods
PubMed ID12531507
'Staining bacteria with esterified fluorogenic substrates followed by flow cytometric analysis offers a means for rapid detection of metabolically active bacteria. Flow cytometry (FCM) was used to assess carboxyfluorescein diacetate (CFDA) and carboxyfluorescein diacetate succinimidyl ester (CFDA/SE) as indicators of bacterial activity for cultured bacteria, including Aeromonas hydrophila, Bacillus subtilis, ... More
In search of rat stem Leydig cells: identification, isolation, and lineage-specific development.
AuthorsGe RS, Dong Q, Sottas CM, Papadopoulos V, Zirkin BR, Hardy MP
JournalProc Natl Acad Sci U S A
PubMed ID16467141
'Leydig cells (LCs) are thought to differentiate from spindle-shaped precursor cells that exhibit some aspects of differentiated function, including 3beta-hydroxysteroid dehydrogenase (3betaHSD) activity. The precursor cells ultimately derive from undifferentiated stem LCs (SLCs), which are postulated to be present in testes before the onset of precursor cell differentiation. We searched ... More
Flow cytometric assessment of allopurinol susceptibility in Leishmania infantum promastigote.
AuthorsKamau SW, Hurtado M, Müller-Doblies UU, Grimm F, Nunez R
JournalCytometry
PubMed ID10918286
'BACKGROUND: Leishmaniasis is a major tropical and subtropical parasitic disease. Sodium stibogluconate, N-methyl -D-glucamine antimoniate, amphotericin B, pentamidine, and ketoconazole are drugs used to treat this disease. Some of these drugs cause severe adverse side effects and treatment failures are common. Allopurinol, a purine analog, has been used to treat ... More
Measuring lymphocyte proliferation, survival and differentiation using CFSE time-series data.
'Cellular proliferation is an essential feature of the adaptive immune response. The introduction of the division tracking dye carboxyfluorescein diacetate succinimidyl ester (CFSE) has made it possible to monitor the number of cell divisions during proliferation and to examine the relationship between proliferation and differentiation. Although qualitative examination of CFSE ... More
IL-18 enhances IL-4 production by ligand-activated NKT lymphocytes: a pro-Th2 effect of IL-18 exerted through NKT cells.
AuthorsLeite-De-Moraes MC, Hameg A, Pacilio M, Koezuka Y, Taniguchi M, Van Kaer L, Schneider E, Dy M, Herbelin A
JournalJ Immunol
PubMed ID11145671
'NKT cells are a remarkably versatile population whose functional capacities are determined by cytokines present in their microenvironment. In this study, we provide evidence for a new immunoregulatory effect of the proinflammatory cytokine IL-18 on NKT cells. We found that IL-18, mainly known for its involvement in NK cell activation ... More
Cutting edge: egress of newly generated plasma cells from peripheral lymph nodes depends on beta 2 integrin.
AuthorsPabst O, Peters T, Czeloth N, Bernhardt G, Scharffetter-Kochanek K, Förster R
JournalJ Immunol
PubMed ID15944247
'During humoral immune responses, naive B cells differentiate into Ab-secreting plasma cells within secondary lymphoid organs. Differentiating plasma cells egress from their sites of generation and redistribute to other tissues, predominantly the bone marrow and mucosal tissues. In this study, we demonstrate that within peripheral lymph nodes newly generated plasma ... More
Deletion of Plasmodium berghei-specific CD4+ T cells adoptively transferred into recipient mice after challenge with homologous parasite.
AuthorsHirunpetcharat C, Good MF
JournalProc Natl Acad Sci U S A
PubMed ID9465082
'The immune response to malaria parasites includes T cell responses that reduce parasites by effector T cell responses and by providing help for antibody responses. Some parasites are more sensitive to antibody and others are more sensitive to cell-mediated immunity. We demonstrate that cultured CD4(+) T cells that produce interferon ... More
Interaction of the selectin ligand PSGL-1 with chemokines CCL21 and CCL19 facilitates efficient homing of T cells to secondary lymphoid organs.
AuthorsVeerman KM, Williams MJ, Uchimura K, Singer MS, Merzaban JS, Naus S, Carlow DA, Owen P, Rivera-Nieves J, Rosen SD, Ziltener HJ
JournalNat Immunol
PubMed ID17401367
'P-selectin glycoprotein ligand 1 (PSGL-1) is central to the trafficking of immune effector cells to areas of inflammation through direct interactions with P-selectin, E-selectin and L-selectin. Here we show that PSGL-1 was also required for efficient homing of resting T cells to secondary lymphoid organs but functioned independently of selectin ... More
Treatment with anti-LFA-1 delays the CD8+ cytotoxic-T-lymphocyte response and viral clearance in mice with primary respiratory syncytial virus infection.
AuthorsRutigliano JA, Johnson TR, Hollinger TN, Fischer JE, Aung S, Graham BS
JournalJ Virol
PubMed ID14990720
'Cytotoxic T lymphocytes (CTLs) play an important role in the immune response against respiratory syncytial virus (RSV) infection. The cell surface molecule lymphocyte function-associated antigen 1 (LFA-1) is an important contributor to CTL activation, CTL-mediated direct cell lysis, and lymphocyte migration. In an attempt to determine the role of LFA-1 ... More
Aberrant T cell differentiation in the absence of Dicer.
AuthorsMuljo SA, Ansel KM, Kanellopoulou C, Livingston DM, Rao A, Rajewsky K
JournalJ Exp Med
PubMed ID16009718
'Dicer is an RNaseIII-like enzyme that is required for generating short interfering RNAs and microRNAs. The latter have been implicated in regulating cell fate determination in invertebrates and vertebrates. To test the requirement for Dicer in cell-lineage decisions in a mammalian organism, we have generated a conditional allele of dicer-1 ... More
Microenvironmental contaminations induced by fluorescent lipophilic dyes used for noninvasive in vitro and in vivo cell tracking.
AuthorsLassailly F, Griessinger E, Bonnet D,
JournalBlood
PubMed ID20215639
'Determining how normal and leukemic stem cells behave in vivo, in a dynamic and noninvasive way, remains a major challenge. Most optical tracking technologies rely on the use of fluorescent or bioluminescent reporter genes, which need to be stably expressed in the cells of interest. Because gene transfer in primary ... More
Analysis of HCV-specific T cells by flow cytometry.
AuthorsShiina M, Rehermann B,
JournalMethods Mol Biol
PubMed ID19009279
'Flow cytometry has become an essential research tool because of the increase in the number of its {applications.} The development of an increasing number of monoclonal antibodies (mAbs) and fluorochromes, and of instruments capable of multicolor detection, allows the acquisition of a large amount of phenotypic and functional information in ... More
CD36 or alphavbeta3 and alphavbeta5 integrins are not essential for MHC class I cross-presentation of cell-associated antigen by CD8 alpha+ murine dendritic cells.
AuthorsSchulz O, Pennington DJ, Hodivala-Dilke K, Febbraio M, Reis e Sousa C
JournalJ Immunol
PubMed ID12055214
'Cross-presentation of cell-associated Ag is thought to involve receptor-mediated uptake of apoptotic cells by dendritic cells (DC), and studies with human DC strongly implicate the endocytic receptor CD36 and the integrins alpha(v)beta(3) and/or alpha(v)beta(5) in this process. In the mouse, cross-presentation was recently shown to be a function of CD8alpha(+) ... More
Cell tracing dyes significantly change single cell mechanics.
AuthorsLulevich V, Shih YP, Lo SH, Liu GY,
JournalJ Phys Chem B
PubMed ID19366241
'Cell tracing dyes are very frequently utilized in cellular biology research because they provide highly sensitive fluorescent tags that do not compromise cellular functions such as growth and proliferation. In many investigations concerning cellular adhesion and mechanics, fluorescent dyes have been employed with the assumption of little impact on the ... More
Antigen-presenting cells recruited by Brugia malayi induce Th2 differentiation of naïve CD4(+) T cells.
AuthorsLoke P, MacDonald AS, Allen JE
JournalEur J Immunol
PubMed ID10760802
'A key feature of nematode infection is a bias towards a type 2 immune response. To investigate the role that antigen-presenting cells (APC) may play in promoting this bias, we used adherent peritoneal exudate cells (PEC) recruited in response to the filarial nematode Brugia malayi, to stimulate naïve T cells ... More
Influence of 2,3,7,8-tetrachlorodibenzo-p-dioxin on the antigen-presenting activity of dendritic cells.
AuthorsVorderstrasse BA, Dearstyne EA, Kerkvliet NI
JournalToxicol Sci
PubMed ID12604839
'We have previously shown that exposure of mice to 2,3,7, 8-tetrachlorodibenzo-p-dioxin (TCDD) induces activation-like changes in splenic dendritic cells (DC) in the absence of antigen challenge. Since activation of DC reduces their ability to phagocytize antigen, we examined the effects of TCDD on the ability of DC to process and ... More
On the role of APC-activation for in vitro versus in vivo T cell priming.
AuthorsStorni T, Bachmann MF
JournalCell Immunol
PubMed ID14643299
'Professional antigen-presenting cells take up antigens for processing and presentation in association with MHC class I and II molecules. When APCs receive the right stimuli, they undergo a maturation process and migrate to secondary lymphoid organs to trigger T cell activation. In this study, we compared side-by-side in vivo and ... More
Stem cell engraftment at the endosteal niche is specified by the calcium-sensing receptor.
AuthorsAdams GB, Chabner KT, Alley IR, Olson DP, Szczepiorkowski ZM, Poznansky MC, Kos CH, Pollak MR, Brown EM, Scadden DT
JournalNature
PubMed ID16382241
'During mammalian ontogeny, haematopoietic stem cells (HSCs) translocate from the fetal liver to the bone marrow, where haematopoiesis occurs throughout adulthood. Unique features of bone that contribute to a microenvironmental niche for stem cells might include the known high concentration of calcium ions at the HSC-enriched endosteal surface. Cells respond ... More
Carbohydrate supplementation and exercise-induced changes in T-lymphocyte function.
AuthorsGreen KJ, Croaker SJ, Rowbottom DG
JournalJ Appl Physiol
PubMed ID12909602
'Carbohydrate (CHO) ingestion during exercise has been shown to reduce perturbations in immune cell numbers and function, possibly through a reduction in the cortisol response to exercise. We have previously observed that exercise decreases T-lymphocyte responses to mitogen via an increase in cell death of both CD4 and CD8 T ... More
Homing and conversion of murine hematopoietic stem cells to lung.
AuthorsDooner M, Cerny J, Colvin G, Demers D, Pimentel J, Greer D, Abedi M, McAuliffe C, Quesenberry P
JournalBlood Cells Mol Dis
PubMed ID14757412
'The hematopoietic stem cell population, lineage negative-Sca positive (HSC), displays a homing defect into bone marrow (BM) after 48-h exposure to interleukin (IL)-3, IL-6, IL-11, and steel factor [J. Hematother. Stem Cell Res. 11 (2002) 913]. Cytokine treatment of murine marrow leads to reversible alterations in adhesion protein expression, which ... More
The B subunit of Escherichia coli heat labile enterotoxin abrogates oral tolerance, promoting predominantly Th2-type immune responses.
AuthorsPlant A, Williams R, Jackson ME, Williams NA
JournalEur J Immunol
PubMed ID14579287
'Mucosal antigen encounter usually results in a state of systemic non-responsiveness (tolerance). This failure to mount a protective response is a major hurdle to mucosal vaccine development. Hence, the identification of safe and effective mucosal adjuvants promoting protective immunity is of critical importance. The non-toxic B subunit of Escherichia coli ... More
MHC class II tetramers identify peptide-specific human CD4(+) T cells proliferating in response to influenza A antigen.
AuthorsNovak EJ, Liu AW, Nepom GT, Kwok WW
JournalJ Clin Invest
PubMed ID10606632
'Antigen-specific T helper cells present in peripheral blood at very low frequencies are capable of rapid clonal expansion during antigenic challenge. The exquisite specificity of this response provides for activation and expansion of a very select cohort of T cells, a feature we have used to directly identify and quantify ... More
The power of single and multibeam two-photon microscopy for high-resolution and high-speed deep tissue and intravital imaging.
AuthorsNiesner R, Andresen V, Neumann J, Spiecker H, Gunzer M,
JournalBiophys J
PubMed ID17557785
'Two-photon microscopy is indispensable for deep tissue and intravital imaging. However, current technology based on single-beam point scanning has reached sensitivity and speed limits because higher performance requires higher laser power leading to sample degradation. We utilize a multifocal scanhead splitting a laser beam into a line of 64 foci, ... More
The spectrum of lymphoid subsets preferentially recruited into the liver reflects that of resident populations.
AuthorsKlugewitz K, Blumenthal-Barby F, Eulenburg K, Emoto M, Hamann A
JournalImmunol Lett
PubMed ID15158612
'Intrahepatic lymphocytes (IHL) differ phenotypically from cells found in the peripheral blood or in lymphoid organs. The liver contains T-cells that are also found in lymphoid organs but a higher proportion of these T-cells compared to those in lymphoid organs express activation or memory markers and very few naïve T-cells ... More
Cyclic adenosine monophosphate is a key component of regulatory T cell-mediated suppression.
AuthorsBopp T, Becker C, Klein M, Klein-Hessling S, Palmetshofer A, Serfling E, Heib V, Becker M, Kubach J, Schmitt S, Stoll S, Schild H, Staege MS, Stassen M, Jonuleit H, Schmitt E,
JournalJ Exp Med
PubMed ID17502663
'Naturally occurring regulatory T cells (T reg cells) are a thymus-derived subset of T cells, which are crucial for the maintenance of peripheral tolerance by controlling potentially autoreactive T cells. However, the underlying molecular mechanisms of this strictly cell contact-dependent process are still elusive. Here we show that naturally occurring ... More
Survival and homeostatic proliferation of naive peripheral CD4+ T cells in the absence of self peptide:MHC complexes.
AuthorsClarke SR, Rudensky AY
JournalJ Immunol
PubMed ID10946271
'TCR-self peptide:MHC interactions play a critical role in thymic positive selection, yet relatively little is known of their function in the periphery. It has been suggested that continued contact with selecting MHC molecules is necessary for long-term peripheral maintenance of naive T cells. More recent studies have also demonstrated a ... More
Determination of gabapentin in human plasma by capillary electrophoresis with laser-induced fluorescence detection and acetonitrile stacking technique.
AuthorsChang SY, Wang FY
JournalJ Chromatogr B Analyt Technol Biomed Life Sci
PubMed ID14670745
'A sensitive analytical method for gabapentin [1-(aminomethyl) cyclohexaneacetic acid] (GBP) in human plasma based on capillary electrophoretic separation and laser-induced fluorescence (LIF) detection has been developed. 6-Carboxyfluorescein succinimidyl ester (CFSE) was used for precolumn derivatization of the non-fluorescent drug in plasma. Optimal separation and detection were obtained with an electrophoretic ... More
Local expression of B7-H1 promotes organ-specific autoimmunity and transplant rejection.
AuthorsSubudhi SK, Zhou P, Yerian LM, Chin RK, Lo JC, Anders RA, Sun Y, Chen L, Wang Y, Alegre ML, Fu YX
JournalJ Clin Invest
PubMed ID14991067
'A number of studies have suggested B7-H1, a B7 family member, inhibits T cell responses. Therefore, its expression on nonlymphoid tissues has been proposed to prevent T cell-mediated tissue destruction. To test this hypothesis, we generated transgenic mice that expressed B7-H1 on pancreatic islet beta cells. Surprisingly, we observed accelerated ... More
Human mesenchymal stem cells exert potent antitumorigenic effects in a model of Kaposi's sarcoma.
AuthorsKhakoo AY, Pati S, Anderson SA, Reid W, Elshal MF, Rovira II, Nguyen AT, Malide D, Combs CA, Hall G, Zhang J, Raffeld M, Rogers TB, Stetler-Stevenson W, Frank JA, Reitz M, Finkel T,
JournalJ Exp Med
PubMed ID16636132
'Emerging evidence suggests that both human stem cells and mature stromal cells can play an important role in the development and growth of human malignancies. In contrast to these tumor-promoting properties, we observed that in an in vivo model of Kaposi''s sarcoma (KS), intravenously (i.v.) injected human mesenchymal stem cells ... More
Immunological effects of transgenic constitutive expression of the type 1 sphingosine 1-phosphate receptor by mouse lymphocytes.
AuthorsGräler MH, Huang MC, Watson S, Goetzl EJ
JournalJ Immunol
PubMed ID15699128
'The type 1 sphingosine 1-phosphate (S1P) G protein-coupled receptor (S1P1) normally transduces S1P effects on lymph node (LN) egress and tissue migration of naive lymphocytes. We now show that persistent expression of S1P1 by lymphocytes of S1P1-transgenic (Tg) mice suppresses delayed-type hypersensitivity and results in production of significantly more IgE ... More
Lymphoid reservoirs of antigen-specific memory T helper cells.
'How vaccines control the development of antigen-specific effector and memory T helper cells is central to protective immunity but remains poorly understood. Here we found that protein vaccination selected high-affinity, CXCR5+ICOS(hi) follicular B-helper T cells (T(FH) cells) that developed in draining lymphoid tissue to regulate B cell responses. In the ... More
Perforin-dependent elimination of dendritic cells regulates the expansion of antigen-specific CD8+ T cells in vivo.
AuthorsYang J, Huck SP, McHugh RS, Hermans IF, Ronchese F
JournalProc Natl Acad Sci U S A
PubMed ID16373503
'The lifespan and survival of dendritic cells (DC) in vivo are potentially critical to the expansion of T cell immune responses. We have previously reported that DC loaded with specific antigen are rapidly eliminated by cytotoxic T lymphocytes (CTL) in vivo, but the site, mechanism, and consequences of DC elimination ... More
Naïve CTLs require a single brief period of antigenic stimulation for clonal expansion and differentiation.
'In defense of the host, the immune system must often raise an effective cytotoxic T lymphocyte (CTL) response from a small number of clonal precursors. The degree to which activation stimuli regulate the expansion and differentiation of naïve CTLs, however, remains unknown. Using an engineered antigen-presenting cell (APC) system that ... More
Langerhans cells cross-present antigen derived from skin.
AuthorsStoitzner P, Tripp CH, Eberhart A, Price KM, Jung JY, Bursch L, Ronchese F, Romani N
JournalProc Natl Acad Sci U S A
PubMed ID16672373
'Dendritic cells (DC) efficiently cross-present exogenous antigen on MHC class I molecules to CD8+ T cells. However, little is known about cross-presentation by Langerhans cells (LC), the DCs of the epidermis. Therefore, we investigated this issue in detail. Isolated murine LCs were able to cross-present soluble ovalbumin protein on MHC-class ... More
The CD4 molecule on CD8+ T lymphocytes directly enhances the immune response to viral and cellular antigens.
AuthorsKitchen SG, Whitmire JK, Jones NR, Galic Z, Kitchen CM, Ahmed R, Zack JA
JournalProc Natl Acad Sci U S A
PubMed ID15731353
'CD8+ T lymphocytes play a major role in cellular-mediated immune responses to foreign antigen. We have previously demonstrated that costimulation of purified human CD8+ T cells induces de novo expression of the CD4 molecule and that ligation of CD4 on this cell type modulates CD8+ T cell activity in vitro. ... More
A novel strategy for rapid and efficient isolation of human tumor-specific CD4(+) and CD8(+) T-cell clones.
AuthorsLee ST, Liu S, Radvanyi L, Sukhumalchandra P, Molldrem JJ, Wieder ED, Hwu P, Liu YJ, Kwak LW, Lizée G, Neelapu SS,
JournalJ Immunol Methods
PubMed ID17959194
'Adoptive therapy with antigen-specific T cells is a promising approach for the treatment of infectious diseases and cancer. However, cloning of antigen-specific T cells by the traditional approach of limiting dilution is a time-consuming, laborious, and inefficient process. Here, we describe a novel flow cytometric strategy for rapid isolation of ... More
Involvement of inhibitory NKRs in the survival of a subset of memory-phenotype CD8+ T cells.
AuthorsUgolini S, Arpin C, Anfossi N, Walzer T, Cambiaggi A, Förster R, Lipp M, Toes RE, Melief CJ, Marvel J, Vivier E
JournalNat Immunol
PubMed ID11323697
'Inhibitory natural killer receptors (NKRs) such as killer cell immunoglobulin-like receptors (KIRs) in humans and Ly49 molecules in mice are expressed on NK cells and recognize multiple major histocompatibility (MHC) class I proteins. In humans and mice, a subset of CD8+ T cells also expresses NKRs and harbors a memory ... More
Quantification of effector/target conjugation involving natural killer (NK) or lymphokine activated killer (LAK) cells by two-color flow cytometry.
'Precise estimates of the frequency of NK- and LAK-target conjugates were obtained by two-color flow cytometry using hydroethidine and calcein as intracellular labels for target cells and effector cells, respectively. These two dyes can easily be used with a standard single-laser flow cytometer with excellent signal separation and dye retention. ... More
Dissipative metabolic patterns respond during neutrophil transmembrane signaling.
AuthorsPetty HR, Kindzelskii AL
JournalProc Natl Acad Sci U S A
PubMed ID11248046
'Self-organization is a common theme in biology. One mechanism of self-organization is the creation of chemical patterns by the diffusion of chemical reactants and their nonlinear interactions. We have recently observed sustained unidirectional traveling chemical redox [NAD(P)H - NAD(P)(+)] waves within living polarized neutrophils. The present study shows that an ... More
Flow cytometric cell division tracking using nuclei.
AuthorsHasbold J, Hodgkin PD
JournalCytometry
PubMed ID10878566
'BACKGROUND: Labeling cells with 5-(and-6) carboxyfluorescein diacetate succinimidyl ester (CFSE) allows their subsequent division history to be determined by flow cytometry. Whether nuclei isolated from CFSE-labeled cells retain any or sufficient dye to reveal the same division history was unknown. If division tracking in nuclei were possible, it would enable ... More
Allogeneic bone marrow inhibits T-cell activation and clonal expansion in vitro.
AuthorsGeorge JF, Lu A, Thomas JM, Kirklin JK, Pinderski LJ
JournalTransplantation
PubMed ID12865816
'BACKGROUND: Donor bone marrow infusion has long been used to enhance graft survival or induce tolerance in T cell depleted solid organ allograft recipients. However, the mechanisms through which bone marrow cells affect tolerance remain obscure. We studied the affect of allogeneic bone marrow cells on the activation of allospecific ... More
Convergent differentiation: myeloid and lymphoid pathways to murine plasmacytoid dendritic cells.
AuthorsSathe P, Vremec D, Wu L, Corcoran L, Shortman K
JournalBlood
PubMed ID23053574
'The developmental origin of IFN-producing plasmacytoid dendritic cells (pDCs) has been uncertain. In the present study, we tracked the development of pDCs in cultures of BM precursors stimulated with Flt3 ligand. Common myeloid precursors (CMPs) produced both conventional DCs (cDCs) and pDCs via the DC-restricted common DC precursor. Common lymphoid ... More
Interleukin-7-dependent expansion and persistence of melanoma-specific T cells in lymphodepleted mice lead to tumor regression and editing.
AuthorsWang LX, Li R, Yang G, Lim M, O'Hara A, Chu Y, Fox BA, Restifo NP, Urba WJ, Hu HM
JournalCancer Res
PubMed ID16288050
'Active-specific immunotherapy with dendritic cells loaded with peptide derived from the melanoma antigen, gp100, failed to mediate regression of established B16F10 melanoma in normal mice. Dendritic cell vaccination induced activation and subsequent deletion of adoptively transferred naive CD8+ T-cell receptor transgenic (pmel-1) T cells specific for gp100 in normal mice. ... More
Lipopolysaccharide stimulates the proliferation of human CD56+CD3- NK cells: a regulatory role of monocytes and IL-10.
AuthorsGoodier MR, Londei M
JournalJ Immunol
PubMed ID10861046
'NK cells recognize and kill tumor cells and normal cells, and these play an important role in immune defense in cancer, infectious disease, and autoimmunity. NK killing is regulated by positive or negative signals derived from the interaction of surface receptors with ligands on the target cells. However, the mechanisms ... More
CD4+ T cells are required for secondary expansion and memory in CD8+ T lymphocytes.
AuthorsJanssen EM, Lemmens EE, Wolfe T, Christen U, von Herrath MG, Schoenberger SP
JournalNature
PubMed ID12594515
'A long-standing paradox in cellular immunology concerns the conditional requirement for CD4+ T-helper (T(H)) cells in the priming of cytotoxic CD8+ T lymphocyte (CTL) responses in vivo. Whereas CTL responses against certain viruses can be primed in the absence of CD4+ T cells, others, such as those mediated through ''cross-priming'' ... More
Carboxyfluorescein (CFSE) labelling of hepatocytes for short-term localization following intraportal transplantation.
'Renewed interest in the transplantation of isolated hepatocytes into the liver as a potential therapy for liver disease has stimulated the development of methods for the identification of donor cells within the recipient organ. We describe a method for cellular tagging and in vivo identification of intraportally transplanted hepatocytes using ... More
Cerebral microvascular responses to hypercholesterolemia: roles of NADPH oxidase and P-selectin.
AuthorsIshikawa M, Stokes KY, Zhang JH, Nanda A, Granger DN
JournalCirc Res
PubMed ID14670846
'Although hypercholesterolemia is widely accepted as a major risk factor for coronary artery and peripheral vascular diseases, its role in the pathogenesis of stroke is controversial. The objectives of this study were to determine how hypercholesterolemia affects the cerebral microcirculation under resting conditions and after ischemia-reperfusion (I/R). Platelet- and leukocyte-endothelial ... More
Tolerant CD8 T cells induced by multiple injections of peptide antigen show impaired TCR signaling and altered proliferative responses in vitro and in vivo.
AuthorsDubois PM, Pihlgren M, Tomkowiak M, Van Mechelen M, Marvel J
JournalJ Immunol
PubMed ID9820498
'The mechanisms responsible for peripheral CD8 T cell tolerance to foreign Ags remain poorly understood. In this study we have characterized the state of CD8 T cell tolerance induced in F5 TCR transgenic mice by multiple peptide injections in vivo. The tolerant state of CD8 T cells is characterized by ... More
Determination of cell adhesion sites of neuropilin-1.
AuthorsShimizu M, Murakami Y, Suto F, Fujisawa H
JournalJ Cell Biol
PubMed ID10725340
'Neuropilin-1 is a type 1 membrane protein with three distinct functions. First, it can mediate cell adhesion via a heterophilic molecular interaction. Second, in neuronal cells, neuropilin-1 binds the class 3 semaphorins, which are neuronal chemorepellents, and plays a role in the directional guidance of axons. Neuropilin-1 is expected to ... More
A flow-cytometric NK-cytotoxicity assay adapted for use in rat repeated dose toxicity studies.
AuthorsMarcusson-Ståhl M, Cederbrant K
JournalToxicology
PubMed ID14599763
'A recent regulatory document for immunotoxicity testing of new pharmaceutical drugs includes cytotoxic natural killer (NK)-cell function as a required parameter in repeated dose toxicity studies. The classical 51Cr-release assay is the conventional test for cytotoxicity testing but several drawbacks with this assay has increased the demand for new reliable ... More
Imatinib mesylate (STI571) inhibits growth of primitive malignant progenitors in chronic myelogenous leukemia through reversal of abnormally increased proliferation.
'Imatinib mesylate (STI571) is a promising new treatment for chronic myelogenous leukemia (CML). The effect of imatinib mesylate on primitive malignant progenitors in CML has not been evaluated, and it is not clear whether suppression of progenitor growth represents inhibition of increased proliferation, induction of apoptosis, or both. We demonstrated ... More
Functional, phenotypic and molecular characterization of cytokine low-responding circulating CD34+ haemopoietic progenitors.
AuthorsPierelli L, Scambia G, Fattorossi A, Bonanno G, Battaglia A, Rumi C, Marone M, Mozzetti S, Rutella S, Menichella G, Romeo V, Mancuso S, Leone G
JournalBr J Haematol
PubMed ID9753035
'Circulating CD34+ cell populations characterized by a low rate (up to five) or high rate (more than five) of cell divisions were isolated from 8 d cultures in the presence of stem cell factor (SCF), interleukin-3 (IL-3), granulocyte-macrophage colony stimulating factor (GM-CSF), granulocyte colony stimulating factor (G-CSF), erythropoietin (EPO), Flt3 ... More
CD8 T cell competition for dendritic cells in vivo is an early event in activation.
AuthorsWillis RA, Kappler JW, Marrack PC
JournalProc Natl Acad Sci U S A
PubMed ID16880405
'T cell responses against an antigen are often focused on a small fraction of potentially immunogenic determinants, a phenomenon known as immunodominance. Immunodominance can be established at several stages of antigen presentation, including antigen processing, binding of peptides to MHC, and competition between T cells for dendritic cells (DCs). The ... More
Assessment of cumulative allergen-activated lymph node cell proliferation using flow cytometry.
AuthorsHumphreys NE, Dearman RJ, Kimber I
JournalToxicol Sci
PubMed ID12700420
'The murine local lymph node assay (LLNA) is a method for the prospective identification of chemical contact allergens. The current validated protocol assesses lymphocyte proliferation induced in the draining lymph node as a function of in situ incorporation of radiolabeled thymidine. We have explored the potential utility of an alternative ... More
Lymphocytes migrate from the blood into the bronchoalveolar lavage and lung parenchyma in the asthma model of the brown Norway rat.
AuthorsSchuster M, Tschernig T, Krug N, Pabst R
JournalAm J Respir Crit Care Med
PubMed ID10673200
'Lymphocyte migration from the blood into the lung has been suggested as being responsible for the increase of lymphocytes, in particular CD4 T cells, in the bronchoalveolar lavage (BAL) and bronchial mucosa in human asthma, but so far there has been no direct proof. We studied lymphocyte immigration and lymphocyte ... More
In vitro senescence occurring in normal human endothelial cells can be rescued by ectopic telomerase activity.
AuthorsYoung AT, Lakey JR, Murray AG, Mullen JC, Moore RB
JournalTransplant Proc
PubMed ID14611993
'Telomerase activation is a means to delay in vitro replicative senescence in human cells via telomere maintainence; however, this enzymatic activity is virtually absent in almost all normal somatic cells. As a result, cell senesce, leading to an eventual loss of graft function. Aging allografts, either due to cell injury ... More
Diabetes is predicted by the beta cell level of autoantigen.
AuthorsByersdorfer CA, Schweitzer GG, Unanue ER
JournalJ Immunol
PubMed ID16177075
'Two novel transgenic (Tg) strains were created expressing hen egg-white lysozyme (HEL) in a pancreas-specific fashion. RmHP.111 mice had levels of HEL per cell similar to that of the established ILK-3 strain, while RmHP.117 mice had 10-fold lower levels (50,000 molecules per cell). When bred to 3A9 TCR Tg mice, ... More
On the self-referential nature of naive MHC class II-restricted T cells.
AuthorsViret C, He X, Janeway CA
JournalJ Immunol
PubMed ID11086052
'The use of mutant mice expressing a normal MHC class II molecule surface level but a severely restricted self-peptide diversity (H-2Malpha(-/-)) previously revealed that T cells carrying the Ealpha(52-68)-I-A(b) complex-specific 1H3.1 TCR rely on self-peptide(s) recognition for both their peripheral persistence in irradiated hosts and their intrathymic positive selection. Here, ... More
Stimulation of macrophages and neutrophils by complexes of lipopolysaccharide and soluble CD14.
AuthorsHailman E, Vasselon T, Kelley M, Busse LA, Hu MC, Lichenstein HS, Detmers PA, Wright SD
JournalJ Immunol
PubMed ID8666811
'Sensitive responses of monocytes, macrophages, and neutrophils to bacterial LPS require membrane-bound CD14 (mCD14) and a plasma protein called LPS-binding protein (LBP). Cells lacking mCD14 respond to complexes of LPS and soluble CD14 (sCD14); these responses do not require LBP. To determine whether LBP is necessary for responses of mCD14-bearing ... More
An evaluation of cell separation techniques in a model mixed cell population.
AuthorsMurphy SJ, Watt DJ, Jones GE
JournalJ Cell Sci
PubMed ID1429892
'Muscle precursor cells may act not only as a means of inserting normal genes into diseased muscle fibres, in order to correct or alleviate a genetically inherited myopathy, but recent demonstrations have shown they may prove an invaluable tool for the expression of, and systemic dissemination of, non-muscle gene products. ... More
'Cadherins are a family of cell adhesion molecules that exhibit calcium-dependent, homophilic binding. Their function depends on both an HisAlaVal sequence in the first extracellular domain, EC1, and the interaction of a conserved cytoplasmic region with intracellular proteins. T-cadherin is an unusual member of the cadherin family that lacks the ... More
High-resolution tracking of cell division demonstrates differential effects of TH1 and TH2 cytokines on SCF-dependent human mast cell production in vitro: correlation with apoptosis and Kit expression.
AuthorsKulka M, Metcalfe DD
JournalBlood
PubMed ID15367434
'T-helper 1 (TH1) (interferon-gamma [IFN-gamma]) and TH2 (interleukin-4 [IL-4] and IL-5) cytokines have been variably reported to alter human mast cell numbers in complex culture systems. The effects of these cytokines on the kinetics of cell division and cell death are unknown, and their effect on mast cell behavior is ... More