Alterations in apoptosis and epithelial-mesenchymal transformation in an in vitro cleft palate model.
AuthorsGurley JM, Wamsley MS, Sandell LJ
JournalPlast Reconstr Surg
PubMed ID15108882
'The processes of apoptosis and epithelial-mesenchymal transformation have been identified as two major mechanisms by which secondary palatal shelves achieve fusion. The aim of this study was to investigate alterations in these mechanisms by changing the physical distance between paired palatal shelves in an in vitro model of palatogenesis. Wild-type ... More
Epithelial-mesenchymal transformation during palatal fusion: carboxyfluorescein traces cells at light and electron microscopic levels.
AuthorsGriffith CM, Hay ED
JournalDevelopment
PubMed ID1295731
'During the fusion of rodent embryo palatal shelves, the cells of the outer epithelial layer slough off, allowing the cells of the medial edge basal layer to form a midline seam that undergoes epithelial-mesenchymal transformation, as judged by electron microscopy and immunohistochemistry. In this study, we analyze the fate of ... More
Interference with axonin-1 and NrCAM interactions unmasks a floor-plate activity inhibitory for commissural axons.
AuthorsStoeckli ET, Sonderegger P, Pollerberg GE, Landmesser LT
JournalNeuron
PubMed ID9052792
Axonin-1 and NrCAM were previously shown to be involved in the in vivo guidance of commissural growth cones across the floor plate of the embryonic chicken spinal cord. To further characterize their role in axon pathfinding, we developed a two-dimensional coculture system of commissural and floor-plate explants in which it ... More
Increased work in cardiac trabeculae causes decreased mitochondrial NADH fluorescence followed by slow recovery.
AuthorsBrandes R, Bers DM
JournalBiophys J
PubMed ID8842239
The oxidative phosphorylation rate in isolated mitochondria is stimulated by increased [ADP], resulting in decreased [NADH]. In intact hearts, however, increased mechanical work has generally not been shown to cause an increase in [ADP]. Therefore, increased [NADH] has been suggested as an alternative for stimulating the phosphorylation rate. Such a ... More
Regulation of human T lymphocyte coactivation with an alpha4 integrin antagonist peptide.
AuthorsMcIntyre BW, Woodside DG, Caruso DA, Wooten DK, Simon SI, Neelamegham S, Revelle JK, Vanderslice P
JournalJ Immunol
PubMed ID9126978
The cyclic hexapeptide CWLDVC (TBC 772) is an antagonist of alpha4 integrins and a potent inhibitor of lymphocyte interactions with fibronectin, vascular cell adhesion molecule-1, and muscosal vascular addressin cell adhesion molecule-1 (MAdCAM-1). As such, peptide TBC 772 effectively inhibits the activation of freshly isolated human T lymphocytes stimulated with ... More
Analysis of bacterial function by multi-colour fluorescence flow cytometry and single cell sorting.
AuthorsNebe-von-Caron G, Stephens PJ, Hewitt CJ, Powell JR, Badley RA
JournalJ Microbiol Methods
PubMed ID11000436
With the increased awareness of the problems associated with the growth dependent analysis of bacterial populations, direct optical detection methods such as flow cytometry have enjoyed increased popularity over the last few years. Among the analyses discussed here are: (1) Bacterial discrimination from other particles on the basis of nucleic ... More