Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells.
This MEM α is modified as follows:
|• Ribonucleosides |
|• Deoxyribonucleosides |
|• Phenol Red |
MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12
, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. This product is made with Earle’s salts.
MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum
(FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g ⁄ L) and therefore requires a 5-10% CO2
environment to maintain physiological pH.
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