5-(和-6)-羧基 SNARF™-1
5-(和-6)-羧基 SNARF™-1
Invitrogen™

5-(和-6)-羧基 SNARF™-1

不可渗透细胞的 pH 值指示剂(羧基 SNARF™-1 染料)的 pKa 为 ∼7.5,因此有助于测量了解更多信息
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货号数量
C12701 mg
货号 C1270
价格(CNY)
6,242.00
Each
添加至购物车
数量:
1 mg
价格(CNY)
6,242.00
Each
添加至购物车
不可渗透细胞的 pH 值指示剂(羧基 SNARF™-1 染料)的 pKa 为 ∼7.5,因此有助于测量 pH 值 7-8 之间的 pH 值变化。羧基-SNARF™-1 在酸性和碱性条件下分别显示出从黄橙色到深红色荧光的显著 pH 值依赖性发射波长变化。这种 pH 值依赖性使得可利用染料在两种发射波长(通常为 580 nm 和 640 nm)处的荧光强度比定量测定 pH 值。

了解有关离子指示剂(包括钙、钾、pH 值和膜电位指示剂)的更多信息›
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
染料类型pH 值指示剂
数量1 mg
运输条件室温
适用于(设备)荧光显微镜, 流式细胞仪, 微孔板读数仪
产品线SNARF
产品类型pH 值指示剂
Unit SizeEach
内容与储存
室温避光储存。

引用和文献 (43)

引用和文献
Abstract
Simultaneous measurement of intracellular pH and Ca2+ in insulin-secreting cells by spectral imaging microscopy.
Authors:Martínez-Zaguilán R,Gurulé MW,Lynch RM
Journal:The American journal of physiology
PubMed ID:8967445
Measurement of intracellular pH.
Authors:Boyer MJ, Hedley DW
Journal:Methods Cell Biol
PubMed ID:7532259
Fluorescent indicators of ion concentrations.
Authors:Tsien RY
Journal:Methods Cell Biol
PubMed ID:2538708
Comparison of spectrum-shifting intracellular pH probes 5'(and 6')-carboxy-10-dimethylamino-3-hydroxyspiro[7H-benzo[c]xanthene-7, 1'(3'H)-isobenzofuran]-3'-one and 2',7'-biscarboxyethyl-5(and 6)-carboxyfluorescein.
Authors:Owen CS
Journal:Anal Biochem
PubMed ID:1514696
'The dyes carboxy-SNARF-1 and BCECF are fluorescent probes of intracellular pH that exhibit changes in spectral shape upon proton binding which allow one to use measurements of fluorescence at two or more wavelengths in order to measure pH without artifacts associated with variability in dye loading, etc. In evaluating these ... More
Profiling pH changes in the electrospray plume.
Authors:Zhou S, Prebyl BS, Cook KD
Journal:Anal Chem
PubMed ID:12380808
'Laser-induced fluorescence spectrometry is used to profile pH changes as droplets evaporate in an electrospray plume by measuring emission spectra of 2(or 4)-[10-(dimethylamino)-3-oxo-3H-benzo[c]xanthene-7-yl]-benzenedicarboxylic acid (carboxy SNARF-1), a pH-sensitive fluorescent dye. The observed pH changes depend on initial droplet pH and polarity. In some instances, small or negligible changes of pH ... More