AuthorsKrasnow MA, Cumberledge S, Manning G, Herzenberg LA, Nolan GP
JournalScience
PubMed ID1898782
'Use of primary culture cells has been limited by the inability to purify most types of cells, particularly cells from early developmental stages. In whole animal cell sorting (WACS), live cells derived from animals harboring a lacZ transgene are purified according to their level of beta-galactosidase expression with a fluorogenic ... More
UVA-induced apoptosis studied by the new apo/necro-Comet-assay which distinguishes viable, apoptotic and necrotic cells.
AuthorsMorley N, Rapp A, Dittmar H, Salter L, Gould D, Greulich KO, Curnow A,
JournalMutagenesis
PubMed ID16500949
An adaptation of the Comet-assay was developed which enables the discrimination of viable, apoptotic and necrotic single cells by use of the common Annexin-V staining and a dye exclusion test on the cells already embedded in agarose gel on glass slides. Membrane integrity (Ethidium-Homodimer exclusion), cellular esterase activity (Calcein blue-AM) ... More
Advantages and limitations of commonly used methods to assay the molecular permeability of gap junctional intercellular communication.
AuthorsAbbaci M, Barberi-Heyob M, Blondel W, Guillemin F, Didelon J,
JournalBiotechniques
PubMed ID18611167
The role of gap junctional intercellular communication (GJIC) in regulation of normal growth and differentiation is becoming increasingly recognized as a major cellular function. GJIC consists of intercellular exchange of low molecular weight molecules, and is the only means for direct contact between cytoplasms of adjacent animal cells. Disturbances of ... More
Early intermediates in HIV-1 envelope glycoprotein-mediated fusion triggered by CD4 and co-receptor complexes.
AuthorsDimitrov AS, Xiao X, Dimitrov DS, Blumenthal R
JournalJ Biol Chem
PubMed ID11397808
An early step in the process of HIV-1 entry into target cells is the activation of its envelope glycoprotein (GP120-GP41) to a fusogenic state upon binding to target cell CD4 and cognate co-receptor. Incubation of human immunodeficiency virus (HIV)-1 Env-expressing cells with an excess of CD4 and co-recepeptor-bearing target cells ... More
RANK ligand-induced elevation of cytosolic Ca2+ accelerates nuclear translocation of nuclear factor kappa B in osteoclasts.
AuthorsKomarova SV, Pilkington MF, Weidema AF, Dixon SJ, Sims SM
JournalJ Biol Chem
PubMed ID12496256
RANK ligand (RANKL) induces activation of NFkappaB, enhancing the formation, resorptive activity, and survival of osteoclasts. Ca(2+) transduces many signaling events, however, it is not known whether the actions of RANKL involve Ca(2+) signaling. We investigated the effects of RANKL on rat osteoclasts using microspectrofluorimetry and patch clamp. RANKL induced ... More
SARS-CoV-2 Infection of Pluripotent Stem Cell-Derived Human Lung Alveolar Type 2 Cells Elicits a Rapid Epithelial-Intrinsic Inflammatory Response.
Authors
JournalCell Stem Cell
PubMed ID32979316
Single-cell transcriptomic analysis defines the interplay between tumor cells, viral infection, and the microenvironment in nasopharyngeal carcinoma.
Authors
JournalCell Res
PubMed ID32901110
STING and IRF3 in stromal fibroblasts enable sensing of genomic stress in cancer cells to undermine oncolytic viral therapy.
Authors
JournalNat Cell Biol
PubMed ID32483388
Organoids Model Transcriptional Hallmarks of Oncogenic KRAS Activation in Lung Epithelial Progenitor Cells.
Authors
JournalCell Stem Cell
PubMed ID32891189
Generation of mesenchyme free intestinal organoids from human induced pluripotent stem cells.
Authors
JournalNat Commun
PubMed ID31924806
Differentiation of Human Pluripotent Stem Cells into Functional Lung Alveolar Epithelial Cells.
Authors
JournalCell Stem Cell
PubMed ID28965766
Derivation of self-renewing lung alveolar epithelial type II cells from human pluripotent stem cells.
Authors
JournalNat Protoc
PubMed ID31732721
Cross-tissue organization of the fibroblast lineage.
Authors
JournalNature
PubMed ID33981032
BEX1 is an RNA-dependent mediator of cardiomyopathy.
Authors
JournalNat Commun
PubMed ID29192139
Creating CRISPR-responsive smart materials for diagnostics and programmable cargo release.