Cell Painting for cytotoxicity and mode-of-action analysis in primary human hepatocytes
AuthorsEwald J, Titterton K, Bäuerle A. et al.
JournalCell Systems
PubMed ID
Scalable, human-relevant approaches for detecting compound modes of action are needed to improve chemical safety evaluation. Here, we apply image-based profiling (Cell Painting) alongside two cytotoxicity assays in primary human hepatocytes exposed to eight concentrations of 1,085 compounds spanning pharmaceuticals, pesticides, and industrial chemicals. We compared three computational approaches (CellProfiler, ... More