CyQUANT™ LDH Cytotoxicity Assay, absorbance, 1000 assays

CyQUANT™ LDH Cytotoxicity Assay, absorbance, 1000 assays - FAQs

查看更多产品信息 CyQUANT™ LDH Cytotoxicity Assays - FAQs (C20301, C20300, C20303, C20302)

6 个常见问题解答

How many assays can I perform using the CyQUANT LDH Cytotoxicity Assay Kit (Cat. No. C20301)?

The CyQUANT LDH Cytotoxicity Assay Kit (Cat. No. C20301) contains sufficient reagents to perform 1,000 assays in 96-well plates.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the concentration of the LDH Positive Control included in the CyQUANT LDH Cytotoxicity Assay Kit (Cat. Nos. C20300, C20301)?

The concentration of the LDH Positive Control included in the CyQUANT LDH Cytotoxicity Assay Kit (Cat. Nos. C20300, C20301) is proprietary.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I observed a slight color change when I prepared the Reaction Mixture from the CyQUANT LDH Cytotoxicity Assay Kit (Cat. Nos. C20300, C20301) prior to testing my samples. Is this normal?

The CYQUANT LDH Cytotoxicity Assay Kit (Cat. Nos. C20300, C20301) Assay Buffer used to prepare the Reaction Mixture, contains the reaction dye and therefore has some inherent coloration.

The color change should be considerably noticeable with the Positive Control in comparison to the Assay Buffer alone.

Find additional tips, troubleshooting help, and resources within our Cell Viability, Proliferation, Cryopreservation, and Apoptosis Support Center.

Can I collect the media from the CyQUANT LDH Cytotoxicity Assay at different time points during the day and then do the assay later in the day?

We do not recommend doing this. The LDH in the media should be tested as soon as possible to avoid the loss of LDH activity. The reaction mixture is stable for 3 to 4 weeks, stored frozen.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I refrigerate or freeze the collected media from the CyQUANT LDH Cytotoxicity Assay and analyze at a later time?

We do not recommend doing this. Refrigeration or freezing and storage over time would cause the loss of LDH activity.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the CyQUANT LDH Cytotoxicity Assay kit on cells in media with serum?

Serum may contain endogenous LDH that can interfere with the assay. To determine feasibility, we recommend testing a cell-free sample of media and serum only, using the same lot of serum to be used on your samples. If different lots of serum are to be used, each lot must be tested for endogenous LDH.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.