Effect of shear stress on microvessel network formation of endothelial cells with in vitro three-dimensional model.
AuthorsUeda A, Koga M, Ikeda M, Kudo S, Tanishita K
JournalAm J Physiol Heart Circ Physiol
PubMed ID15130887
'Shear stress stimulus is expected to enhance angiogenesis, the formation of microvessels. We determined the effect of shear stress stimulus on three-dimensional microvessel formation in vitro. Bovine pulmonary microvascular endothelial cells were seeded onto collagen gels with basic fibroblast growth factor to make a microvessel formation model. We observed this ... More
Melatonin prevents death of neuroblastoma cells exposed to the Alzheimer amyloid peptide.
AuthorsPappolla MA, Sos M, Omar RA, Bick RJ, Hickson-Bick DL, Reiter RJ, Efthimiopoulos S, Robakis NK
JournalJ Neurosci
PubMed ID9030627
'Studies from several laboratories have generated evidence suggesting that oxidative stress is involved in the pathogenesis of Alzheimer''s disease (AD). The finding that the amyloid beta protein (Abeta) has neurotoxic properties and that such effects are, in part, mediated by free radicals has provided insights into mechanisms of cell death ... More
Fluorescent labelling of intracellular bacteria in living host cells.
AuthorsBoleti H, Ojcius DM, Dautry-Varsat A
JournalJ Microbiol Methods
PubMed ID10802143
The fluorescent reagent, CellTracker, labels metabolically-active cells and was used here to label Chlamydia in vivo during their exponential phase of growth in infected cells. HeLa cells infected with C. psittaci were labelled with the CellTracker reagents between 15 and 48 h post-infection. The fluorescent label accumulated in the host-cell ... More
Transcription factor KLF2 regulates the migration of naive T cells by restricting chemokine receptor expression patterns.
AuthorsSebzda E, Zou Z, Lee JS, Wang T, Kahn ML,
JournalNat Immunol
PubMed ID18246069
The migration patterns of naive and activated T cells are associated with the expression of distinct sets of chemokine receptors, but the molecular basis for this regulation is unknown. Here we identify Krupple-like factor 2 (KLF2) as a key transcriptional factor needed to prevent naive T cells from expressing inflammatory ... More
GAS6 mediates adhesion of cells expressing the receptor tyrosine kinase Axl.
AuthorsMcCloskey P, Fridell YW, Attar E, Villa J, Jin Y, Varnum B, Liu ET
JournalJ Biol Chem
PubMed ID9287338
Axl is a receptor tyrosine kinase that contains both immunoglobulin and fibronectin III repeats in its extracellular domain reminiscent of cell adhesion molecules. Expression of the receptor tyrosine kinase Axl in the 32D myeloid cell line permits aggregation of cells in response to treatment with the native ligand GAS6; this ... More
Induction of CD4 T cell changes in murine AIDS is dependent on costimulation and involves a dysregulation of homeostasis.
AuthorsYen MH, Lepak N, Swain SL
JournalJ Immunol
PubMed ID12097374
Strong CD4 T cell activation and proliferation are seen in susceptible mice infected with the murine retroviral inoculum, LP-BM5, which produces an immunodeficiency syndrome called murine AIDS (MAIDS). We developed a short term adoptive transfer model of MAIDS to examine the requirements for the CD4 T cell response. Naive CD4 ... More
Potent neuroprotective properties against the Alzheimer beta-amyloid by an endogenous melatonin-related indole structure, indole-3-propionic acid.
Widespread cerebral deposition of a 40-43-amino acid peptide called the amyloid beta-protein (Abeta) in the form of amyloid fibrils is one of the most prominent neuropathologic features of Alzheimer's disease. Numerous studies suggest that Abeta is toxic to neurons by free radical-mediated mechanisms. We have previously reported that melatonin prevents ... More
The rostral migratory stream plays a key role in intranasal delivery of drugs into the CNS.
Authors
JournalPLoS One
PubMed ID21533252
SMARTer single cell total RNA sequencing.
Authors
JournalNucleic Acids Res
PubMed ID31216024
Sinupret activates CFTR and TMEM16A-dependent transepithelial chloride transport and improves indicators of mucociliary clearance.
Authors
JournalPLoS One
PubMed ID25117505
Resveratrol enhances airway surface liquid depth in sinonasal epithelium by increasing cystic fibrosis transmembrane conductance regulator open probability.
Authors
JournalPLoS One
PubMed ID24282612
Microglial trogocytosis and the complement system regulate axonal pruning in vivo.
Authors
JournalElife
PubMed ID33724186
LPS decreases CFTR open probability and mucociliary transport through generation of reactive oxygen species.
Authors
JournalRedox Biol
PubMed ID33971543
Internalization of NK cells into tumor cells requires ezrin and leads to programmed cell-in-cell death.
Authors
JournalCell Res
PubMed ID19786985
HO-1 inhibits preadipocyte proliferation and differentiation at the onset of obesity via ROS dependent activation of Akt2.
Authors
JournalSci Rep
PubMed ID28102348
ATG proteins mediate efferocytosis and suppress inflammation in mammary involution.
Authors
JournalAutophagy
PubMed ID23380905
Anti-c-Met antibodies recognising a temperature sensitive epitope, inhibit cell growth.