Hydrolysis of fluorescent pyrene-acyl esters by human pancreatic carboxylic ester hydrolase and bile salt-stimulated lipase.
AuthorsNegre-Salvayre A, Abouakil N, Lombardo D, Salvayre R
JournalLipids
PubMed ID2215086
'Fluorescent esters containing pyrenedecanoic acid (P10) or pyrenebutanoic (P4) acid (P4cholesterol, P10cholesterol, P4- and P10-containing triacylglycerols) were synthesized and used as substrates for human pancreatic carboxylic ester hydrolase and bile salt-stimulated lipase from human milk. Both enzymes were purified by immunoaffinity chromatography. All fluorescent pyrene derivatives were hydrolyzed by pancreatic ... More
A laser spectrofluorimeter for studies of cultured cells on the growth surface.
AuthorsGianturco SH, Hong KY, Steiner MR, Taunton OD, Jackson RL, Gotto AM, Smith LC