The ADF (actin-depolymerizing factor)/cofilin family is a stimulus-responsive mediator of actin dynamics. In contrast to the mechanisms of inactivation of ADF/cofilin by kinases such as LIM-kinase 1 (LIMK1), much less is known about its reactivation through dephosphorylation. Here we report Slingshot (SSH), a family of phosphatases that have the property ... More
Captavidin: a new regenerable biocomponent for biosensing?
AuthorsGarcía-Aljaro C, Muñoz FX, Baldrich E,
JournalAnalyst
PubMed ID19838424
Functionalisation of a biosensor's sensing surface with the appropriate biorecognition elements is essential for the correct performance of the biosensor. In this paper, we investigate by Surface Plasmon Resonance (SPR) if captavidin, a recently described biotin-binding regenerable protein, could be used to bind and release biotinylated biocomponents for the development ... More
Nitroavidin as a ligand for the surface capture and release of biotinylated proteins.
AuthorsBolivar JG, Soper SA, McCarley RL,
JournalAnal Chem
PubMed ID19551994
Discussed here is the preparation, detailed purification, and evaluation of nitroavidin as a ligand for surface capture and release of biotinylated proteins. Avidin from chicken egg white was nitrated using dilute tetranitromethane solutions. UV-vis spectroscopy was used to show decreased binding of the biotin analogue, 2-(4'-hydroxyazobenzene)benzoic acid, HABA, to nitroavidin ... More
Immobilized nitro-avidin and nitro-streptavidin as reusable affinity matrices for application in avidin-biotin technology.
AuthorsMorag E, Bayer EA, Wilchek M
JournalAnal Biochem
PubMed ID8954558
Chemically modified forms of egg-white avidin and bacterial streptavidin (termed nitro-avidin and nitro-streptavidin, respectively), in which the binding-site tyrosine was nitrated, were used for several biotechnological applications. The fundamental difference between nitro-avidin and the native protein is that interaction of the modified protein with biotin can be reversed under relatively ... More