Identification of fetal DNA and cells in skin lesions from women with systemic sclerosis.
AuthorsArtlett CM, Smith JB, Jimenez SA
JournalN Engl J Med
PubMed ID9554859
'BACKGROUND: Systemic sclerosis is a disease of unknown origin which often occurs in women after their childbearing years. It has many clinical and histopathological similarities to chronic graft-versus-host disease. Recent studies indicate that fetal stem cells can survive in the maternal circulation for many years post partum. This finding suggests ... More
Immunomagnetic colloids for the enrichment of tumor cells from peripheral blood and bone marrow: a model system.
AuthorsKemshead JT, Hancock J, Liberti P
JournalJ Hematother
PubMed ID7922009
'The characterization of tumor cells in bone marrow harvested for autologous bone marrow rescue remains a problem due to the limited sensitivities of the techniques available for their analysis. This complicates the use of purging techniques as their usefulness is debated, specifically because it is not known if they can ... More
Detection and characterization of carcinoma cells in the blood.
'A highly sensitive assay combining immunomagnetic enrichment with multiparameter flow cytometric and immunocytochemical analysis has been developed to detect, enumerate, and characterize carcinoma cells in the blood. The assay can detect one epithelial cell or less in 1 ml of blood. Peripheral blood (10-20 ml) from 30 patients with carcinoma ... More
Magnetic labeling and cell sorting.
AuthorsOwen CS, Sykes NL
JournalJ Immunol Methods
PubMed ID6491303
'Magnetic labeling by a magnetite-antibody conjugate has been combined with magnetic filtration (high gradient magnetic separation) to effect a rapid and efficient separation of a selected cell population from a suspension of single cells. Samples of more than 10(8) cells could be fractionated in about 5 min with complete recovery. ... More
A magnetic colloid system for isolation of rare cells from blood for fish analysis.
AuthorsMartin B, Bruce J, Smith N, Liu D, Durrant L
JournalPrenat Diagn
PubMed ID9399355
To improve cell recovery of trophoblast and nucleated red blood cells from maternal blood for diagnosis of chromosomal abnormalities, we have investigated the use of a magnetic sorting system that utilizes a ferrofluid. The main advantage of this system is that the beads used are small enough to allow visualization ... More
Antibody-magnetite method for selective concentration of Giardia lamblia cysts from water samples.
AuthorsBifulco JM, Schaefer FW
JournalAppl Environ Microbiol
PubMed ID8481003
An antibody-magnetite method was developed in order to selectively concentrate Giardia cysts from water samples. The indirect technique employed a mouse immunoglobulin G anti-Giardia antibody as a primary antibody and an anti-mouse immunoglobulin G antibody-coated magnetite particle as a secondary labeling reagent. The magnetically labeled cysts were then concentrated by ... More
Flow cytometry--principles and feasibility in transfusion medicine. Enumeration of epithelial derived tumor cells in peripheral blood.
AuthorsTerstappen LW, Rao C, Gross S, Kotelnikov V, Racilla E, Uhr J, Weiss A
JournalVox Sang
PubMed ID9704456
Immunomagnetic selection of epithelial cells from peripheral blood was combined with flowcytometric or microscopic analysis to characterize and enumerate epithelium derived tumor cells in the circulation of patients with carcinoma of the breast. Highly significant differences in the number of circulating epithelial cells were found between normal controls and patients ... More
Magnetic sorting of leukocytes.
AuthorsOwen CS
JournalCell Biophys
PubMed ID2429773
Magnetic filtration of surface-labeled cells has been applied to the fractionation of leukocytes in a model system, using a colloidal magnetite reagent to label mouse spleen cells. This reagent was completely free from problems of aggregation or settling. Since the individual submicron particles were invisible under the microscope, cells were ... More
A rapid and highly selective approach to cell separations using an immunomagnetic colloid.
AuthorsHancock JP, Kemshead JT
JournalJ Immunol Methods
PubMed ID7689621
Immunomagnetic colloids have the properties of solutions and, therefore, offer distinct advantages in their ability to bind to cells as compared to larger magnetic microspheres. B cells and T cells have been isolated with higher degrees of purity following incubation with monoclonal antibodies (MoAbs) and a goat anti-mouse Ig ferrofluid. ... More
Optical tracking and detection of immunomagnetically selected and aligned cells.
AuthorsTibbe AG, de Grooth BG, Greve J, Liberti PA, Dolan GJ, Terstappen LW
JournalNat Biotechnol
PubMed ID10585720
We have developed a platform for cell analysis based on immunomagnetic selection and magnetic alignment of cells in combination with an epi-illumination tracking and detection system. Whole blood was labeled with ferromagnetic nanoparticles and fluorescent probes, and placed in a magnetic field in a chamber. Cells labeled with ferromagnetic nanoparticles ... More
Prenatal diagnosis using fetal cells isolated from maternal peripheral blood: a review.
AuthorsSteele CD, Wapner RJ, Smith JB, Haynes MK, Jackson LG
JournalClin Obstet Gynecol
PubMed ID8934032
Many questions remain about the feasibility of using fetal cells from maternal blood for prenatal diagnosis. Although recently there has been more focus on clinically relevant methods, many studies have been performed using blood drawn after invasive procedures, and over a wide range of gestational ages. For methods to be ... More