Measuring, in solution, multiple-fluorophore labeling by combining fluorescence correlation spectroscopy and photobleaching.
AuthorsDelon A, Wang I, Lambert E, Mache S, Mache R, Derouard J, Motto-Ros V, Galland R,
JournalJ Phys Chem B
PubMed ID20143802
Determining the number of fluorescent entities that are coupled to a given molecule (DNA, protein, etc.) is a key point of numerous biological studies, especially those based on a single molecule approach. Reliable methods are important, in this context, not only to characterize the labeling process but also to quantify ... More