The role of sarcolemmal Ca2+-ATPase in the regulation of resting calcium concentration in rat ventricular myocytes.
AuthorsChoi HS, Eisner DA
JournalJ Physiol
PubMed ID9925882
'1. The aim of this work was to investigate the role of sarcolemmal Ca2+-ATPase in rat ventricular myocytes. We have measured intracellular Ca2+ concentration ([Ca2+]i) using indo-1. The actions of the ATPase inhibitor carboxyeosin were studied. 2. Carboxyeosin increased resting [Ca2+]i and the magnitude of the systolic Ca2+ transient and ... More
Effects on the hepatocyte [Ca2+]i oscillator of inhibition of the plasma membrane Ca2+ pump by carboxyeosin or glucagon-(19-29).
AuthorsGreen AK, Cobbold PH, Dixon CJ
JournalCell Calcium
PubMed ID9292228
'Single rat hepatocytes, microinjected with the Ca(2+)-sensitive photoprotein aequorin, respond to agonists acting through the phosphoinositide signalling pathway by the generation of oscillations in cytosolic free Ca2+ concentration ([Ca2+]i). The duration of [Ca2+]i transients generated is characteristic of the receptor species activated; the variability results in differences in the rate ... More
Inhibitory interaction of the plasma membrane Na+/Ca2+ exchangers with the 14-3-3 proteins.
AuthorsPulina MV, Rizzuto R, Brini M, Carafoli E
JournalJ Biol Chem
PubMed ID16679322
The three Na+/Ca2+ exchanger isoforms, NCX1, NCX2, and NCX3, contain a large cytoplasmic loop that is responsible for the regulation of activity. We have used 347 residues of the loop of NCX2 as the bait in a yeast two-hybrid approach to identify proteins that could interact with the exchanger and ... More
Reduced contraction strength with increased intracellular [Ca2+] in left ventricular trabeculae from failing rat hearts.
AuthorsWard ML, Pope AJ, Loiselle DS, Cannell MB
JournalJ Physiol
PubMed ID12527740
Intracellular calcium ([Ca2+](i)) and isometric force were measured in left ventricular (LV) trabeculae from spontaneously hypertensive rats (SHR) with failing hearts and normotensive Wistar-Kyoto (WKY) controls. At a physiological stimulation frequency (5 Hz), and at 37 degrees C, the peak stress of SHR trabeculae was significantly (P < or = ... More