霍乱毒素亚基 B(重组),Alexa Fluor™ 555偶联物
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霍乱毒素亚基 B(重组),Alexa Fluor™ 555偶联物
Invitrogen™

霍乱毒素亚基 B(重组),Alexa Fluor™ 555偶联物

Molecular Probes™ 霍乱毒素偶联物仅由重组形式的 B 亚基组成。这使我们能够提供完全不含有毒 A 亚基的高纯度产品。霍乱毒素 B了解更多信息
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货号数量
C22843500μg
C34776100μg
货号 C22843
价格(CNY)
9,942.00
飞享价
Ends: 31-Dec-2025
13,477.00
共减 3,535.00 (26%)
Each
添加至购物车
数量:
500μg
价格(CNY)
9,942.00
飞享价
Ends: 31-Dec-2025
13,477.00
共减 3,535.00 (26%)
Each
添加至购物车
Molecular Probes™ 霍乱毒素偶联物仅由重组形式的 B 亚基组成。这使我们能够提供完全不含有毒 A 亚基的高纯度产品。霍乱毒素 B 亚基 (CT-B) 通过结合神经节苷脂 GM1 附着在细胞上,是强大的神经元逆行标记工具。这种示踪剂已用于多种应用领域,包括追踪大鼠前脑传入、臂旁区域投射及膀胱壁神经元。在神经元示踪应用中使用时,CT-B 通常通过压力注射或通过离子电渗注入神经组织的方式注入。

霍乱毒素 B 亚基规格:
• 标记(激发/发射波长):Alexa Fluor™ 555 (555/565 nm)
• 在中性 pH 下,11.4 kDa B 亚基以 57kDa 五聚体形式存在
• 冻干产品可使用缓冲液(如 PBS)溶解使用



研究人员最近发现,CT-B 可用作脂筏标记物——脂筏是一种膜微结构域,在胆固醇和鞘脂质中含量丰富,被视作研究细胞信号转导的重要工具。脂筏染色时,首先将细胞与荧光素 CT-B 一同孵育,然后加入抗–CT-B 抗体,使脂筏中的 CT-B 交联成质膜上的不同斑块。这些斑块可通过荧光显微镜轻松观察到。除单独的荧光 CT-B 偶联物外,我们还提供含有 Alexa Fluor™ 488Alexa Fluor™ 555Alexa Fluor™ 594 染料的 CT-B 偶联物、抗 –CT-B 抗体及荧光显微检测细胞标记和制备的详细实验方案的 Vybrant™ 脂筏标记试剂盒。

查找更多的 CT-B 偶联物的信息
我们提供了多种 CT-B 偶联物。有关这类示踪剂的更多信息、请参阅《Molecular Probes™ 手册》中的“蛋白偶联物—第14.7节”。

仅供科研使用。不可用于人或动物的治疗或诊断。
仅供科研使用。不可用于诊断程序。
规格
标签类型Alexa Fluor 染料
产品线Alexa Fluor
蛋白质形式Recombinant
蛋白质子类型霍乱毒素
数量500μg
运输条件室温
偶联物Alexa Fluor 555
形式Lyophilized
重组Recombinant
Unit SizeEach
内容与储存
储存在冰箱(-5 至 -30°C)中并避光。

引用和文献 (27)

引用和文献
Abstract
Autoantigen Golgin-97, an effector of Arl1 GTPase, participates in traffic from the endosome to the trans-golgi network.
Authors:Lu L, Tai G, Hong W
Journal:Mol Biol Cell
PubMed ID:15269279
'The precise cellular function of Arl1 and its effectors, the GRIP domain Golgins, is not resolved, despite our recent understanding that Arl1 regulates the membrane recruitment of these Golgins. In this report, we describe our functional study of Golgin-97. Using a Shiga toxin B fragment (STxB)-based in vitro transport assay, ... More
Caveolin regulates endocytosis of the muscle repair protein, dysferlin.
Authors:Hernández-Deviez DJ, Howes MT, Laval SH, Bushby K, Hancock JF, Parton RG,
Journal:J Biol Chem
PubMed ID:18096699
'Dysferlin and Caveolin-3 are plasma membrane proteins associated with muscular dystrophy. Patients with mutations in the CAV3 gene show dysferlin mislocalization in muscle cells. By utilizing caveolin-null cells, expression of caveolin mutants, and different mutants of dysferlin, we have dissected the site of action of caveolin with respect to dysferlin ... More
Integrin-mediated adhesion regulates membrane order.
Authors:Gaus K, Le Lay S, Balasubramanian N, Schwartz MA
Journal:J Cell Biol
PubMed ID:16943184
'The properties of cholesterol-dependent domains (lipid rafts) in cell membranes have been controversial. Because integrin-mediated cell adhesion and caveolin both regulate trafficking of raft components, we investigated the effects of adhesion and caveolin on membrane order. The fluorescent probe Laurdan and two-photon microscopy revealed that focal adhesions are highly ordered; ... More
Metastatic potential of mouse Lewis lung cancer cells is regulated via ganglioside GM1 by modulating the matrix metalloprotease-9 localization in lipid rafts.
Authors:Zhang Q, Furukawa K, Chen HH, Sakakibara T, Urano T, Furukawa K
Journal:J Biol Chem
PubMed ID:16636068
'To analyze mechanisms for cancer metastasis, we established high metastatic sublines from mouse Lewis lung cancer (P29) by repeated injection. Sublines established from the two subclones H7 and C4 commonly exhibited increased proliferation and invasion activity and reduced expression of ganglioside GM1, although they showed different preferences in their target ... More
Quantitative and dynamic assessment of the contribution of the ER to phagosome formation.
Authors:Touret N, Paroutis P, Terebiznik M, Harrison RE, Trombetta S, Pypaert M, Chow A, Jiang A, Shaw J, Yip C, Moore HP, van der Wel N, Houben D, Peters PJ, de Chastellier C, Mellman I, Grinstein S,
Journal:Cell
PubMed ID:16213220
'Phagosomes were traditionally thought to originate from an invagination and scission of the plasma membrane to form a distinct intracellular vacuole. An alternative model implicating the endoplasmic reticulum (ER) as a major component of nascent and maturing phagosomes was recently proposed (Gagnon et al., 2002). To reconcile these seemingly disparate ... More