An assay for intermolecular exchange of alpha crystallin.
AuthorsGopalakrishnan S, Takemoto L
JournalInvest Ophthalmol Vis Sci
PubMed ID1526743
An affinity column of alpha crystallin linked to cyanogen bromide-activated Sepharose was developed to study the exchange of alpha subunits. Alpha crystallin bound to the Sepharose-alpha complex was dissociated with 8 mol/l urea, followed by quantitation using high-performance reverse-phase liquid chromatography. The time course of binding at 37 degrees C ... More