Differential effect of N-carboxymethylisatoylation on the DNA polymerase activity, the 5' leads to 3'-exonuclease activity and the 3' leads to 5'-exonuclease activity of DNA polymerase I of Escherichia coli.
AuthorsKlenow H, Overgaard-Hansen K
JournalBiochim Biophys Acta
PubMed ID6269620
'Treatment with native DNA polymerase I of Escherichia coli with the acylating agent N-carboxymethylisatoic acid anhydride (NCMIA) results under specific conditions in a rapid loss of polymerase activity, an increase in 5'' leads to 3''-exonuclease activity and in unchanged 3'' leads to 5''-exonuclease activity. When a nucleoside triphosphate and Mg2+ ... More
Kinetics of interaction between normal and proline 12 Ras and the GTPase-activating proteins, p120-GAP and neurofibromin. The significance of the intrinsic GTPase rate in determining the transforming ability of ras.
AuthorsEccleston JF, Moore KJ, Morgan L, Skinner RH, Lowe PN
JournalJ Biol Chem
PubMed ID8262937
Single turnover and equilibrium binding measurements on the interaction of Gly-12 and Pro-12 Ras.GTP with the catalytic domains of the GTPase-activating proteins, p120-GAP and neurofibromin, have been made utilizing fluorescent 2'(3')O-(N-methylanthraniloyl)-nucleotides. These have enabled the equilibrium dissociation constants (Kd) for their initial binding and the rate constants of the hydrolysis ... More