Differential efficacy of lipophilic and cytosolic antioxidants on generation of reactive oxygen species by amyloid-beta.
AuthorsDhitavat S, Rivera ER, Rogers E, Shea TB
JournalJ Alzheimers Dis
PubMed ID12214018
'Exposure of neurons to amyloid-beta (Abeta) is accompanied by a cascade of oxidative damage that initiates with lipid peroxidation followed by subsequent generation of cytosolic free radicals and reactive oxygen species (ROS). The antioxidant vitamin E has been utilized to counteract Abeta-induced oxidative stress. We considered herein whether or not ... More
Mitochondrial localization of reactive oxygen species by dihydrofluorescein probes.
AuthorsDiaz G, Liu S, Isola R, Diana A, Falchi AM
JournalHistochem Cell Biol
PubMed ID14574587
'Mitochondria are the main source of reactive oxygen species (ROS). The aim of this work was to verify the ROS generation in situ in HeLa cells exposed to prooxidants and antioxidants (menadione, tert-butyl hydroperoxide, antimycin A, vitamin E, N-acetyl-L-cysteine, and butylated hydroxytoluene) using the ROS-sensitive probes 6-carboxy-2'',7''-dichlorodihydrofluorescein diacetate di-acetomethyl ester ... More
NAD(P)H oxidase mediates the endothelial barrier dysfunction induced by TNF-alpha.
AuthorsGertzberg N, Neumann P, Rizzo V, Johnson A
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID12807699
'We tested the hypothesis that the NAD(P)H oxidase-dependent generation of superoxide anion (O2-*) mediates tumor necrosis factor-alpha (TNF)-induced alterations in the permeability of pulmonary microvessel endothelial monolayers (PMEM). The permeability of PMEM was assessed by the clearance rate of Evans blue-labeled albumin. The NAD(P)H oxidase subcomponents p47phox and p22phox were ... More
Prevention by anti-LFA-1 of acute myoblast death following transplantation.
AuthorsGuérette B, Skuk D, Célestin F, Huard C, Tardif F, Asselin I, Roy B, Goulet M, Roy R, Entman M, Tremblay JP
JournalJ Immunol
PubMed ID9278346
'Myoblast transplantation is a potential treatment for Duchenne muscular dystrophy. One of the problems possibly responsible for the limited success of clinical trials is the rapid death of the myoblasts after transplantation. To investigate this problem, myoblasts expressing beta-galactosidase were injected in the tibialis anterior muscles of mice. Beta-galactosidase activity ... More
Implication of mitochondrial hydrogen peroxide generation in ceramide-induced apoptosis.
AuthorsQuillet-Mary A, Jaffrézou JP, Mansat V, Bordier C, Naval J, Laurent G
JournalJ Biol Chem
PubMed ID9261153
'The key events implicated in ceramide-triggered apoptosis remain unknown. In this study we show that 25 microM C6-ceramide induced significant H2O2 production within 60 min, which increased up to 180 min in human myeloid leukemia U937 cells. Inactive analogue dihydro-C6-ceramide had no effect. Furthermore, no H2O2 production was observed in ... More
Quantitative multiwell myeloid differentiation assay using dichlorodihydrofluorescein diacetate (H2DCF-DA) or dihydrorhodamine 123 (H2R123).
AuthorsTrayner ID, Rayner AP, Freeman GE, Farzaneh F
JournalJ Immunol Methods
PubMed ID7594627
'It is well established that the fluorescent probes dichlorodihydrofluorescein diacetate (H2DCF-DA) and dihydrorhodamine 123 (H2R123) can be used to detect the respiratory burst response of mature myeloid cells. We describe a simple, fast and quantitative assay for myeloid differentiation based on the oxidation of these probes, which can be performed ... More
Reduction in the E2k subunit of the alpha-ketoglutarate dehydrogenase complex has effects independent of complex activity.
AuthorsShi Q, Chen HL, Xu H, Gibson GE
JournalJ Biol Chem
PubMed ID15649899
'The activity of the alpha-ketoglutarate dehydrogenase complex (KGDHC) declines in brains of patients with several neurodegenerative diseases. KGDHC consists of multiple copies of E1k, E2k, and E3. E1k and E2k are unique to KGDHC and may have functions independent of the complex. The present study tested the consequences of different ... More
Hyperoxia-induced NAD(P)H oxidase activation and regulation by MAP kinases in human lung endothelial cells.
AuthorsParinandi NL, Kleinberg MA, Usatyuk PV, Cummings RJ, Pennathur A, Cardounel AJ, Zweier JL, Garcia JG, Natarajan V
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID12388366
'Hyperoxia increases reactive oxygen species (ROS) production in vascular endothelium; however, the mechanisms involved in ROS generation are not well characterized. We determined the role and regulation of NAD(P)H oxidase in hyperoxia-induced ROS formation in human pulmonary artery endothelial cells (HPAECs). Exposure of HPAECs to hyperoxia for 1, 3, and ... More
Free radical-independent protection by nerve growth factor and Bcl-2 of PC12 cells from hydrogen peroxide-triggered apoptosis.
AuthorsSatoh T, Sakai N, Enokido Y, Uchiyama Y, Hatanaka H
JournalJ Biochem (Tokyo)
PubMed ID8902618
'To investigate the mechanism of oxidative stress induced death of PC12 cells, we performed confocal and flow cytometric analysis with a reactive oxygen species (ROS)-specific fluorogen, 6-carboxy-2'',7''-dichlorodihydrofluorescein diacetate, di(acetoxymethyl ester) (C-DCDHF-DA). Hydrogen peroxide significantly decreased the number of viable PC12 cells after 24 h. Hydrogen peroxide caused membrane blebbing, nuclear ... More
Survival factor-insensitive generation of reactive oxygen species induced by serum deprivation in neuronal cells.
AuthorsSatoh T, Sakai N, Enokido Y, Uchiyama Y, Hatanaka H
JournalBrain Res
PubMed ID8891242
'To investigate the involvement of reactive oxygen species (ROS) in neuronal apoptosis, we performed confocal and flow cytometric analysis with a ROS-specific fluorogen, 6-carboxy-2'', 7''-dichorodihydrofluorescein diacetate, di(acetoxymethyl ester) (C-DCDHF-DA). Serum deprivation significantly increased the level of ROS in PC12 cells and rat cortical neurons. N,N''-diphenyl-p-phenylenediamine (DPPD), an antioxidant, reduced ROS ... More
Detecting enzymatic activity in cells using fluorogenic substrates.
AuthorsHaugland RP
JournalBiotech Histochem
PubMed ID8580208
Fluorogenic substrates can detect enzymatic activity associated with cells. It is difficult, however, to detect activity within a single cell or in an organelle since hydrolytic substrates yield products that rapidly leak from the cell. Several new solutions are presented including trapping the fluorescent product in membranes, in cell organelles, ... More
Long-term two-photon fluorescence imaging of mammalian embryos without compromising viability.
AuthorsSquirrell JM, Wokosin DL, White JG, Bavister BD
JournalNat Biotechnol
PubMed ID10429240
A major challenge for fluorescence imaging of living mammalian cells is maintaining viability following prolonged exposure to excitation illumination. We have monitored the dynamics of mitochondrial distribution in hamster embryos at frequent intervals over 24 h using two-photon microscopy (1,047 nm) while maintaining blastocyst, and even fetal, developmental competence. In ... More
Role of Bim in diallyl trisulfide-induced cytotoxicity in human cancer cells.
AuthorsLee BC, Park BH, Kim SY, Lee YJ,
JournalJ Cell Biochem
PubMed ID21053278
The aim of this study was to investigate the effect of garlic constituent diallyl trisulfide (DATS) on the cell-death signaling pathway in a human breast cell line (MDA-MB-231). We observed that DATS (10-100?µM) treatment resulted in dose- and time-dependent cytotoxicity. Treatment of MDA-MB-231 cells with a cytotoxicity inducing concentration of ... More
Chelation of lysosomal iron protects dopaminergic SH-SY5Y neuroblastoma cells from hydrogen peroxide toxicity by precluding autophagy and Akt dephosphorylation.
AuthorsCastino R, Fiorentino I, Cagnin M, Giovia A, Isidoro C,
JournalToxicol Sci
PubMed ID21742779
In human neuroblastoma SH-SY5Y cells, hydrogen peroxide (H(2)O(2), 200µM) rapidly (< 5 min) induced autophagy, as shown by processing and vacuolar relocation of light chain 3(LC3). Accumulation of autophagosome peaked at 30 min of H(2)O(2) exposure. The continuous presence of H(2)O(2) eventually (at > 60 min) caused autophagy-dependent annexin V-positive ... More
Bcl-2 functions in an antioxidant pathway to prevent apoptosis.
Bcl-2 inhibits most types of apoptotic cell death, implying a common mechanism of lethality. Bcl-2 is localized to intracellular sites of oxygen free radical generation including mitochondria, endoplasmic reticula, and nuclear membranes. Antioxidants that scavenge peroxides, N-acetylcysteine and glutathione peroxidase, countered apoptotic death, while manganese superoxide dismutase did not. Bcl-2 ... More
Activation of flavin-containing oxidases underlies light-induced production of H2O2 in mammalian cells.
AuthorsHockberger PE, Skimina TA, Centonze VE, Lavin C, Chu S, Dadras S, Reddy JK, White JG
JournalProc Natl Acad Sci U S A
PubMed ID10339574
Violet-blue light is toxic to mammalian cells, and this toxicity has been linked with cellular production of H2O2. In this report, we show that violet-blue light, as well as UVA, stimulated H2O2 production in cultured mouse, monkey, and human cells. We found that H2O2 originated in peroxisomes and mitochondria, and ... More
Low glutathione and high iron govern the susceptibility of oligodendroglial precursors to oxidative stress.
AuthorsThorburne SK, Juurlink BH
JournalJ Neurochem
PubMed ID8752107
We have previously shown, using qualitative approaches, that oligodendroglial precursors are more readily damaged by free radicals than are astrocytes. In the present investigation we quantified the oxidative stress experienced by the cells using oxidation of dichlorofluorescin diacetate to dichlorofluorescein as a measure of oxidative stress; furthermore, we have delineated ... More
Production of reactive oxygen species and release of L-glutamate during superoxide anion-induced cell death of cerebellar granule neurons.
AuthorsSatoh T, Numakawa T, Abiru Y, Yamagata T, Ishikawa Y, Enokido Y, Hatanaka H
JournalJ Neurochem
PubMed ID9422377
Enhanced production of superoxide anion (O2-) is considered to play a pivotal role in the pathogenesis of CNS neurons. Here, we report that O2- generated by xanthine (XA) + xanthine oxidase (XO) triggered cell death associated with nuclear condensation and DNA fragmentation in cerebellar granule neuron. XA + XO induced ... More
Participation of reactive oxygen species in PGF2alpha-induced apoptosis in rat luteal cells.
AuthorsTanaka M, Miyazaki T, Tanigaki S, Kasai K, Minegishi K, Miyakoshi K, Ishimoto H, Yoshimura Y
JournalJ Reprod Fertil
PubMed ID11058439
Prostaglandin F(2alpha) (PGF(2alpha)) is implicated in the process of luteal regression in many species. Treatment of rat luteal tissue with PGF(2alpha) increases the generation of reactive oxygen species. Since reactive oxygen species have been implicated in apoptosis, the present study was undertaken to determine whether reactive oxygen species play a ... More
Suppression of endothelial cell apoptosis by high density lipoproteins (HDL) and HDL-associated lysosphingolipids.
AuthorsNofer JR, Levkau B, Wolinska I, Junker R, Fobker M, von Eckardstein A, Seedorf U, Assmann G
JournalJ Biol Chem
PubMed ID11432865
Apoptotic cell death following injury of vascular endothelium is assumed to play an important role in the pathogenesis of atherosclerosis. In this report, we demonstrate that high density lipoproteins (HDL), a major anti-atherogenic lipoprotein fraction, protect endothelial cells against growth factor deprivation-induced apoptosis. HDL blocked the mitochondrial pathway of apoptosis ... More
Molecular mechanism of glutathione-mediated protection from oxidized low-density lipoprotein-induced cell injury in human macrophages: role of glutathione reductase and glutaredoxin.
AuthorsWang Y, Qiao M, Mieyal JJ, Asmis LM, Asmis R
JournalFree Radic Biol Med
PubMed ID16895798
Macrophage death is a hallmark of advanced atherosclerotic plaque, and oxidized low-density lipoprotein (OxLDL) found in these lesions is believed to contribute to macrophage injury. However, the underlying mechanisms of this phenomenon are only poorly understood. Here we show that in human monocyte-derived macrophages, OxLDL depleted intracellular glutathione (GSH) and ... More
Activation of AMP-activated protein kinase reduces hyperglycemia-induced mitochondrial reactive oxygen species production and promotes mitochondrial biogenesis in human umbilical vein endothelial cells.
AuthorsKukidome D, Nishikawa T, Sonoda K, Imoto K, Fujisawa K, Yano M, Motoshima H, Taguchi T, Matsumura T, Araki E
JournalDiabetes
PubMed ID16380484
We previously proposed that the production of hyperglycemia-induced mitochondrial reactive oxygen species (mtROS) is a key event in the development of diabetes complications. The association between the pathogenesis of diabetes and its complications and mitochondrial biogenesis has been recently reported. Because metformin has been reported to exert a possible additional ... More
Hyperoxia alters phorbol ester-induced phospholipase D activation in bovine lung microvascular endothelial cells.
AuthorsRoy S, Parinandi N, Zeigelstein R, Hu Q, Pei Y, Travers JB, Natarajan V
JournalAntioxid Redox Signal
PubMed ID12716481
We investigated the effect of hyperoxia on phospholipase D (PLD) activation in bovine lung microvascular endothelial cells (BLMVECs). Generation of intracellular reactive oxygen species in BLMVECs exposed to hyperoxia for 2 or 24 h was three-fold higher compared with normoxic cells as measured by dichlorodihydrofluorescein di(acetoxymethyl ester) fluorescence. Exposure of ... More
alpha(4)-integrin mediates neutrophil-induced free radical injury to cardiac myocytes.
AuthorsPoon BY, Ward CA, Cooper CB, Giles WR, Burns AR, Kubes P
JournalJ Cell Biol
PubMed ID11238444
Previous work has demonstrated that circulating neutrophils (polymorphonuclear leukocytes [PMNs]) adhere to cardiac myocytes via beta(2)-integrins and cause cellular injury via the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase enzyme system. Since PMNs induced to leave the vasculature (emigrated PMNs) express the alpha(4)-integrin, we asked whether (a) these PMNs also induce ... More
Flow cytometric analysis of the granulocyte respiratory burst: a comparison study of fluorescent probes.
AuthorsVowells SJ, Sekhsaria S, Malech HL, Shalit M, Fleisher TA
JournalJ Immunol Methods
PubMed ID7829869
Chronic granulomatous disease (CGD) is a rare recessive disorder caused by defects in the NADPH oxidase enzyme complex of phagocytes (neutrophils, eosinophils and monocytes). CGD phagocytes fail to produce superoxide and other reactive oxygen species following cell activation (Malech, 1993). The products of oxidase activation can be measured in individual ... More
Adult motor neuron apoptosis is mediated by nitric oxide and Fas death receptor linked by DNA damage and p53 activation.
AuthorsMartin LJ, Chen K, Liu Z
JournalJ Neurosci
PubMed ID16000635
The mechanisms of injury- and disease-related degeneration of motor neurons (MNs) need clarification. Unilateral avulsion of the sciatic nerve in the mouse induces apoptosis of spinal MNs that is p53 and Bax dependent. We tested the hypothesis that MN apoptosis is Fas death receptor dependent and triggered by nitric oxide ... More
Roxadustat Does Not Affect Platelet Production, Activation, and Thrombosis Formation.
Authors
JournalArterioscler Thromb Vasc Biol
PubMed ID34348487
ROS-mediated upregulation of Noxa overcomes chemoresistance in chronic lymphocytic leukemia.
Authors
JournalOncogene
PubMed ID20935673
Reactive oxygen species and lipoxygenases regulate the oncogenicity of NPM-ALK-positive anaplastic large cell lymphomas.
Authors
JournalOncogene
PubMed ID19503098
Phenolic antioxidants trolox and caffeic acid modulate the oxidized LDL-induced EGF-receptor activation.
Authors
JournalBr J Pharmacol
PubMed ID11309250
Mesenchymal stromal cells protect cancer cells from ROS-induced apoptosis and enhance the Warburg effect by secreting STC1.
Authors
JournalMol Ther
PubMed ID22146344
Loss of HSPA9 induces peroxisomal degradation by increasing pexophagy.
Authors
JournalAutophagy
PubMed ID31964216
Inhibition of SGK1 confers vulnerability to redox dysregulation in cervical cancer.
Authors
JournalRedox Biol
PubMed ID31136958
DNA-SWCNT Biosensors Allow Real-Time Monitoring of Therapeutic Responses in Pancreatic Ductal Adenocarcinoma.
Authors
JournalCancer Res
PubMed ID31292162
CAML regulates Bim-dependent thymocyte death.
Authors
JournalCell Death Differ
PubMed ID20300112
A Delicate Balance between Bacterial Iron and Reactive Oxygen Species Supports Optimal C. elegans Development.