Search
引用和文献 (156)
Invitrogen™
腔肠素
腔肠素和合成腔肠素类似物可用于在已被水母发光蛋白 cDNA 转染的细胞中重组水母发光蛋白。了解更多有关离子指示剂（包括钙、钾、pH 值和膜电位指示剂）的信息›了解更多信息
| 货号 | 数量 |
|---|---|
| C2944 | 250 μg |
货号 C2944
价格（CNY)
4,869.00
Each
数量:
250 μg
价格（CNY)
4,869.00
Each
仅供科研使用。不可用于诊断程序。
规格
检测方法生物发光
染料类型基于蛋白质
数量250 μg
运输条件室温
产品类型腔肠素
Unit SizeEach
内容与储存
在冷冻冰箱（-5°C 至 -30°C）中避光储存。
引用和文献 (156)
引用和文献
Abstract
Monitoring dynamic changes in free Ca2+ concentration in the endoplasmic reticulum of intact cells.
Journal:EMBO J
PubMed ID:8521803
'Direct monitoring of the free Ca2+ concentration in the lumen of the endoplasmic reticulum (ER) is an important but still unsolved experimental problem. We have shown that a Ca(2+)-sensitive photoprotein, aequorin, can be addressed to defined subcellular compartments by adding the appropriate targeting sequences. By engineering a new aequorin chimera
Differential gel electrophoresis and transgenic mitochondrial calcium reporters demonstrate spatiotemporal filtering in calcium control of mitochondria.
Journal:J Biol Chem
PubMed ID:16670086
'Mitochondria must adjust both their intracellular location and their metabolism in order to balance their output to the needs of the cell. Here we show by the proteomic technique of time series difference gel electrophoresis that a major result of neuroendocrine stimulation of the Drosophila renal tubule is an extensive
Crystal structure of a Ca2+-discharged photoprotein: implications for mechanisms of the calcium trigger and bioluminescence.
Journal:J Biol Chem
PubMed ID:15155735
'Ca2+-regulated photoproteins are members of the EF-hand calcium-binding protein family. The addition of Ca2+ produces a blue bioluminescence by triggering a decarboxylation reaction of protein-bound hydroperoxycoelenterazine to form the product, coelenteramide, in an excited state. Based on the spatial structures of aequorin and several obelins, we have postulated mechanisms for
Cotranslational formation of active photoprotein obelin in a cell-free translation system: direct ultrahigh sensitive measure of the translation course.
Journal:Anal Biochem
PubMed ID:10036164
'Translation of apoobelin mRNA in a cell-free wheat germ translation system in the presence of coelenterazine and molecular oxygen results in cotranslational formation of active photoprotein. Active obelin formation is recorded by its luminescence, either direct in the translation mixture in the presence of coelenterazine and calcium ions or in
Measurement of changes in sarcoplasmic reticulum [Ca2+] in rat tail artery with targeted apoaequorin delivered by an adenoviral vector.
Journal:Cell Calcium
PubMed ID:9056079
'The physiologic relevance of Ca2+ release from the sarcoplasmic reticulum in arterial smooth muscle contraction is controversial. Therefore, we sought to measure changes in sarcoplasmic reticulum free [Ca2+] (i.e. [Ca2+]sr) in the intact rat tail artery. We exploited a novel technique to measure [Ca2+]sr with genetically targeted apoaequorin acting as